scholarly journals Converting a Face-to-Face Lab to Online: An Example of Process and Outcomes for a CRISPR-Based Molecular Biology Lab

CourseSource ◽  
2021 ◽  
Vol 8 ◽  
Author(s):  
Lisa M. McDonnell ◽  
Keefe Reuther ◽  
Andrew Cooper ◽  
Christopher Day ◽  
Cindy Gustafson-Brown
Keyword(s):  
Molecular Biology ◽  
Face To Face ◽  
Biology Lab

scholarly journals Toxic remains: Infrastructural failure in a Ugandan molecular biology lab

2021 ◽  
pp. 030631272110115
Author(s):  
Sandra Calkins
Keyword(s):  
Molecular Biology ◽  
Hazardous Chemicals ◽  
Biology Lab

This article complicates romances of infrastructural improvisation by describing infrastructural failures that expose researchers to hazardous chemicals in a Ugandan molecular biology lab. To meet project deadlines, to make careers and to participate in transnational collaborative projects, Ugandan biologists have to stand in for decaying or absent infrastructures with their bodies. Ugandan biologists hide such sacrifices from their international scientific partners and direct the blame elsewhere. An unclear culpability results precisely from the ways in which power works and is distributed across transnational scientific infrastructures.

scholarly journals Using restriction mapping to teach basic skills in the molecular biology lab

2007 ◽  
Vol 35 (3) ◽  
pp. 199-205 ◽  
Author(s):  
Lauren Walsh ◽  
Elizabeth Shaker ◽  
Elizabeth A. De Stasio
Keyword(s):  
Molecular Biology ◽  
Basic Skills ◽  
Restriction Mapping ◽  
Biology Lab

Commentary: Innovation in the biochemistry/molecular biology lab

2009 ◽  
Vol 37 (1) ◽  
pp. 24-25 ◽  
Author(s):  
Rodney Boyer ◽  
Adele Wolfson
Keyword(s):  
Molecular Biology ◽  
Biology Lab

scholarly journals If They Build It

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Bryan Wang
Keyword(s):  
Molecular Biology ◽  
Learning Experiences ◽  
Laboratory Work ◽  
Biology Lab ◽  
Scientific Ideas

In an undergraduate biochemistry and molecular biology lab course, students designed their own final assignment to communicate their laboratory work to non-disciplinary audiences. A “meta-assignment” guided them as they proposed the content, form, and process requirements. Students strove to develop unique ideas, and all successfully completed their self-assigned projects. Providing students in this class with the freedom, responsibility, and appropriate scaffolding to build their own projects and learning experiences allowed them to interact with their discipline in new ways and enhanced their abilities to design and plan their work, communicate scientific ideas to nonscientists, and think creatively.

scholarly journals Protocols for Northern Analysis of Exosome Substrates and Other Noncoding RNAs

2019 ◽  
pp. 83-103
Author(s):  
Cristina Cruz ◽  
Jonathan Houseley
Keyword(s):  
Molecular Biology ◽  
Sequence Data ◽  
Noncoding Rnas ◽  
Northern Blotting ◽  
Northern Analysis ◽  
Standard Laboratory ◽  
Agarose Gels ◽  
The Past ◽  
Technical Advances ◽  
Biology Lab

AbstractOver the past decade a plethora of noncoding RNAs (ncRNAs) have been identified, initiating an explosion in RNA research. Although RNA sequencing methods provide unsurpassed insights into ncRNA distribution and expression, detailed information on structure and processing are harder to extract from sequence data. In contrast, northern blotting methods provide uniquely detailed insights into complex RNA populations but are rarely employed outside specialist RNA research groups. Such techniques are generally considered difficult for nonspecialists, which is unfortunate as substantial technical advances in the past few decades have solved the major challenges. Here we present simple, reproducible and highly robust protocols for separating glyoxylated RNA on agarose gels and heat denatured RNA on polyacrylamide–urea gels using standard laboratory electrophoresis equipment. We also provide reliable transfer and hybridization protocols that do not require optimization for most applications. Together, these should allow any molecular biology lab to elucidate the structure and processing of ncRNAs of interest.

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