The study of corneal biomechanics has gained interest due to its applications on predicting refractive surgery outcomes and the study of a number of pathologies affecting the cornea. In this regard, non-contact tonometry (NCT) has become a popular diagnostic tool in ophthalmology and as an alternative method to characterize corneal biomechanics. Since identification of material parameters using NCT tests rely on the inverse finite element method, accurate and reliable simulations are required. In this work, we present a full fluid structure simulation of a NCT test accounting for the eff ect of the presence of the humors. The results indicate that when inertial effects are considered, not including humors may lead to overestimating corneal displacement, and therefore, to an overestimation of the actual corneal stiffness when using the inverse finite element method.