Fluid Flow
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2021 ◽  
marcus pollard ◽  
Rhushabh Maugi ◽  
Angelika Holzinger ◽  
Micheal Scanlon ◽  
Mark Platt

Resistive pulse sensors have been used to characterise everything from whole cells to small molecules. Their integration into microfluidic devices have simplified sample handling whilst increasing throughput. Typically, these devices measure a limited size range or a specific analyte, making them prone to blockages in complex sample matrixes. To prolong their life and facilitate their use, samples are often filtered or prepared to match the sample with the sensor diameter. Here, we advance our tuneable flow resistive pulse sensor which utilises additively manufactured parts. The sensor allows parts to be easily changed, washed and cleaned, its simplicity and versatility allows components from existing nanopore fabrication techniques such as silicon nitride, polyurethane and glass pipettes to be integrated into a single device. This creates a multi-nanopore sensor that can simultaneously measure particles from 0.1 to 30 m in diameter. The orientation and controlled fluid flow in the device allows the sensors to be placed in series, whereby smaller particles can be measured in the presence of larger ones without the risk of being blocked. We demonstrate the device with a range of nanopore materials commonly found within the literature, the easiest to set up was the pulled glass pipette and glass nanopore membrane. However, the glass nanopore membrane was by far the most robust and reusable component tested. We illustrate the concept of a multi-pore flow resistive pulse sensor, by combining an additively manufactured tuneable sensor, termed sensor 1, with a fixed nanopore sensor, termed sensor 2. Sensor 1 measures particles 2 to 30 m in diameter, whilst sensor 2 can be used to characterise particles as small as 100 nm, depending upon its dimensions.

2022 ◽  
Vol 413 ◽  
pp. 126635
Cristina Vaghi ◽  
Sebastien Benzekry ◽  
Clair Poignard

2022 ◽  
Vol 258 ◽  
pp. 106680
Mikail F. Lumentut ◽  
Michael I. Friswell

Micha Sam Brickman Raredon ◽  
Alexander James Engler ◽  
Yifan Yuan ◽  
Allison Marie Greaney ◽  
Laura E. Niklason

In recent years, it has become common to experiment with ex vivo perfused lungs for organ transplantation, and to attempt regenerative pulmonary engineering using decellularized lung matrices. However, our understanding of the physiology of ex vivo organ perfusion is imperfect: it is not currently well understood how decreasing microvascular barrier affects the perfusion of pulmonary parenchyma. Additionally, protocols for lung perfusion and organ culture fluid-handling are far from standardized, with widespread variation on both basic methods and on ideally controlled parameters. To address both of these deficits, a robust, non-invasive, and mechanistic model is needed which is able to predict microvascular resistance and permeability in perfused lungs while providing insight into capillary recruitment. Although validated mathematical models exist for fluid flow in native pulmonary tissue, previous models generally assume minimal intravascular leak from artery to vein and do not assess capillary bed recruitment. Such models are difficult to apply to both ex vivo lung perfusions, in which edema can develop over time and microvessels can become blocked, and to decellularized ex vivo organomimetic cultures, in which microvascular recruitment is variable and arterially-perfused fluid enters into the alveolar space. Here, we develop a mathematical model of pulmonary microvascular fluid flow which is applicable in both instances, and we apply our model to data from native, decellularized, and regenerating lungs under ex vivo perfusion. The results provide substantial insight into microvascular pressure-flow mechanics, while producing previously unknown output values for tissue-specific capillary-alveolar hydraulic conductivity, microvascular recruitment, and total organ barrier resistance.

Heat Transfer ◽  
2021 ◽  
Kora Lalitha ◽  
Yarranna Veeranna ◽  
Giriyajjara Sreenivasa ◽  
Deshmukh Ashok Reddy

Ling-zhi Yang ◽  
Hang Hu ◽  
Ze-shi Yang ◽  
Bo-tao Xue ◽  
Yu-feng Guo ◽  

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Soumia Manaa ◽  
Salah Boulaaras ◽  
Hamid Benseridi ◽  
Mourad Dilmi ◽  
Sultan Alodhaibi

In this paper, we consider the Brinkman equation in the three-dimensional thin domain ℚ ε ⊂ ℝ 3 . The purpose of this paper is to evaluate the asymptotic convergence of a fluid flow in a stationary regime. Firstly, we expose the variational formulation of the posed problem. Then, we presented the problem in transpose form and prove different inequalities for the solution u ε , p ε independently of the parameter ε . Finally, these estimates allow us to have the limit problem and the Reynolds equation and establish the uniqueness of the solution.

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