scholarly journals Microvascular disorders induced by malaria infected red blood cells: a computational mechanical study using the biological particle method

2009 ◽  
Author(s):  
T. Yamaguchi ◽  
H. Kondo ◽  
Y. Imai ◽  
T. Ishikawa
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Particle Method ◽  
Mechanical Study ◽  
Biological Particle

Particle Method Simulation of Red Blood Cells Infected by Malaria

2009 ◽  
pp. 393-394
Author(s):  
Takami Yamaguchi ◽  
Young Ho Kang ◽  
Hitoshi Kondo ◽  
Yohsuke Imai ◽  
Takuji Ishikawa
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Particle Method

Simulation study on effects of elastic red blood cells on primary thrombogenesis using particle method

2006 ◽  
Vol 39 ◽  
pp. S430-S431
Author(s):  
K.-i. Tsubota ◽  
H. Kamada ◽  
S. Wada ◽  
T. Yamaguchi
Keyword(s):  
Red Blood Cells ◽  
Simulation Study ◽  
Blood Cells ◽  
Particle Method

Simulation of Red Blood Cell Mechanical Behavior in Optical Tweezers Experiment Based on a Particle Method

2010 ◽  
Author(s):  
M. T. Ahmadian ◽  
K. Firoozbakhsh ◽  
M. Hasanian
Keyword(s):  
Mechanical Behavior ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Experimental Studies ◽  
Particle Method ◽  
Living Cells ◽  
Discrete Models ◽  
Particle Methods ◽  
External Forces

Optical tweezers provide an accurate measurement technique for evaluating mechanical properties of the living cells and many experimental studies have been done to understand the behavior of cells due to external forces. Numerical studies such as finite element methods have been used in order to simulate mechanical behavior of the Red Blood Cells (RBCs). Recent studies have shown that the particle methods are useful tools to simulate the mechanical behavior of living cells. Since in microscopic scales, using discrete models are preferred than continuum methods, a particle-based method is used to simulate the deformation of RBC which is stretched by optical tweezers. The cytoplasm of RBC is modeled as a fluid and cell membrane is replaced by a set of discrete particles connected by springs. The results are comparable with previous observations of RBC optical tweezers experiments. It was observed that RBC viscoelastic characteristics are mainly associated with the cytoplasm fluidic properties. In order to understand the behavior and function of living red blood cells, this significant developed model could be implemented to RBC interaction within micocapillaries and constricted zones in blood flow.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

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