A Comparison of Acti*Tip™ Allerg*E™ and Allerg*ENS™ System to Phadebas RAST® and Phadebas IgE PRIST® in Assaying Total IgE and Specific IgE in Human Serum

1988 ◽  
Vol 2 (3) ◽  
pp. 135-140 ◽  
Author(s):  
John T. Connell

Two in vitro systems of measuring total IgE and specific IgE antibodies were compared using serum of patients suspected of having allergic rhinitis due to inhalation of grass pollen. One in vitro system was the Phadebas PRIST and RAST. The accuracy of these tests has been well defined and they were used as the standard. The Acti*Tip Allerg*E and Allerg*ENS system is a new technique utilizing a similar solid-phase immunologic methodology with an enzyme-based detection system. It has some advantages primarily related to the mechanics of performing the test and marked reduction in time for obtaining results. The correlation between the Phadebas and Acti*Tip systems was highly significant for measuring total IgE and specific IgE antibodies for grasses in serum.

1995 ◽  
Vol 95 (3) ◽  
pp. 668-671 ◽  
Author(s):  
Cristina Pascual ◽  
Jesus F. Crespo ◽  
Joaquin Quiralte ◽  
Concepcion Lopez ◽  
Gary Wheeler ◽  
...  

1983 ◽  
Vol 11 (3) ◽  
pp. 194-197 ◽  
Author(s):  
B. A. Baldo ◽  
M. McD. Fisher

Although anaphylactoid reactions to muscle-relaxant drugs are now well recognised, there has been no general agreement on the mechanism(s) underlying the responses. By covalently coupling alcuronium and tubocurarine to a solid phase carrier and using the resultant complexes in radioimmunoassay experiments with patients’ sera and 125I-anti-human IgE, we have found high levels of drug-specific IgE antibodies in the sera of some subjects who reacted to these muscle relaxants. These results provide important supporting evidence that, in some patients at least, lifethreatening anaphylactoid reactions to muscle-relaxant drugs are mediated by drug-specific IgE antibodies.


2021 ◽  
Author(s):  
Huimin Huang ◽  
Zhifeng Huang ◽  
Peiyan Zheng ◽  
Nili Wei ◽  
Xueqing Liang ◽  
...  

Based on the current urgent need for an in vitro quantitative detection system for allergens in most hospitals in China, we introduced a novel allergen-specific immunoglobulin E detection system that...


2011 ◽  
Vol 8 (4) ◽  
pp. 55-61
Author(s):  
N G Konyukova ◽  
A N Pampura ◽  
E S Fedenko ◽  
A I Khavkin ◽  
T S Okuneva ◽  
...  

Background. To establish the clinical and immunological features of the manifestations of allergic diseases in children with IgE-mediated sensitization to potato. Methods. allergy tests (skin prick tests and / or the determination of specific IgE in serum by ImmunoCap, Phadia was performed in 171 children with allergic diseases, who had sensitization to food plant proteins. Results. sensitization to the potato found in 134 children. significant discrepancies (36%) in the results of in vitro and in vivo tests to the potatoes were shown. sensitization to the potatoes is more common in patients with atopic dermatitis (ad), especially in severe disease. among children with the level of specific IgE antibodies to potato ≥2 kUa / l there were more common patients with severe ad. the correlation between the level of specific IgE antibodies to the potatoes and some of food plant proteins was found. the relationship between the concentration of specific IgE to the potatoes and Bet v 2 was not detected. Conclusion. the presence and concentration of specific IgE to the potatoes to a certain extent associated with clinical manifestations of ad. the data obtained substantiate the determination of specific IgE antibodies to potato in children with ad, especially in severe cases.


2000 ◽  
Vol 30 (9) ◽  
pp. 1298-1306 ◽  
Author(s):  
Åkesson ◽  
Ingvarsson ◽  
Brady ◽  
Moynagh ◽  
Borrebaeck

RADIOISOTOPES ◽  
1990 ◽  
Vol 39 (10) ◽  
pp. 445-448
Author(s):  
Akio YOSHIMOTO ◽  
Mitsuyoshi YAZAKI ◽  
Emi SUGIYAMA ◽  
Takao SUGAYA ◽  
Masatoshi AKUTSU ◽  
...  

2008 ◽  
Vol 121 (2) ◽  
pp. S190-S190
Author(s):  
C MAYORGA ◽  
M TORRES ◽  
N BLANCALOPEZ ◽  
J RODRIGUEZBADA ◽  
E PEREZINESTROSA ◽  
...  

2016 ◽  
Vol 46 ◽  
pp. 7-8
Author(s):  
Elecia B. Johnston ◽  
Sandip D. Kamath ◽  
Jennifer Rolland ◽  
Robyn O'Hehir ◽  
Patrick Schaeffer ◽  
...  

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