scholarly journals Standardization of zygotic embryo culture from Nerium oleander L. and comparative analysis of biosynthesized cardiac glycosides within in vitro and acclimatized plants

2021 ◽  
pp. 204-215
Author(s):  
Renu Nimoriya ◽  
Yatendra Singh ◽  
Sumit Kumar Singh ◽  
Pankaj Singh ◽  
Amar Jeet ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Cardiac Glycoside ◽  
Cardiac Glycosides ◽  
Zygotic Embryo ◽  
Germination Percentage ◽  
Zygotic Embryos ◽  
In Vitro Multiplication ◽  
Zygotic Embryo Culture ◽  
In Vitro Plantlets

The primary result of our experiment revealed that the germination percentage of N. oleander mature seeds is only 30%. From this observation, the concept of protocol standardization for zygotic embryo culture of this plant was originated. Zygotic embryo culture was proved an efficient in vitro multiplication system of N. oleander. The maximum germination percentage (96%) of zygotic embryos was observed on ¼ MS medium with 15 gm/L sucrose, whereas the best growth medium was optimized as ½ B5 with same sucrose concentration. The second part of this study was aimed to find out the cardiac glycoside accumulation pattern in both in vitro and acclimatized plants. For this purpose, one-month-old in vitro plantlets and acclimatized plants were subjected to LC-MS analysis and 09 cardiac glycosides were detected and quantified in both the systems. Most of the cardiac glycosides including odoroside A (32.71 mg/gm DW), odoroside H (4.69 mg/gm DW) and oleandrin (0.52 mg/gm DW) were found to be accumulated at maximum level within in vitro plantlets. CG 840b (1.89 mg/gm DW) is the only cardiac glycoside, which was maximally accumulated in acclimatized plants. From this study, it can be concluded that, zygotic embryo culture is a better choice for in vitro multiplication of N. oleander when compared to matured seeds and in vitro grown plantlets of this species favor cardiac glycosides biosynthesis in comparison to acclimatized plants. Therefore, all future research on the enrichment of cardiac glycosides from this plant may be conducted on zygotic embryos derived in vitro grown plantlets or cultures.

scholarly journals In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth

2012 ◽  
Vol 64 (2) ◽  
pp. 503-509 ◽  
Author(s):  
Dragana Stojicic ◽  
Dusica Janosevic ◽  
Branka Uzelac ◽  
V. Cokesa ◽  
Snezana Budimir
Keyword(s):  
Seedling Growth ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
Zygotic Embryos ◽  
Physical Factors ◽  
Zygotic Embryo Culture ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
Pinus Peuce

This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.

Zygotic embryo culture is an efficient way to optimize in vitro growth in Panax ginseng

2021 ◽  
Vol 167 ◽  
pp. 113497
Author(s):  
Jung-Woo Lee ◽  
Gyung-Ran Do ◽  
Ic-Hyun Jo ◽  
Chi-Eun Hong ◽  
Kyung-Hwan Bang ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
In Vitro Growth ◽  
Zygotic Embryo Culture

scholarly journals Reproductive Characteristics of the Selected Cocoa (Theobroma cacao L.) Clones after Regenerated from the Somatic Embryogenesis Culture

2021 ◽  
Vol 18 (24) ◽  
pp. 1406
Author(s):  
Gibson Entuni ◽  
Hollena Nori ◽  
Rebicca Edward ◽  
Ahmad Kamil bin Mohammad Jaafar
Keyword(s):  
Somatic Embryogenesis ◽  
Reproductive Cycle ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
Reproductive Organ ◽  
Immature Zygotic Embryo ◽  
Reproductive Characteristics ◽  
Zygotic Embryo Culture ◽  
Typical Period ◽  
Immature Zygotic Embryo Culture

This study was conducted to evaluate the reproductive characteristics of 4 elite cocoa clones (MCBC1, PBC230, KKM22 and KKM4) propagated via somatic embryogenesis culture. From the findings, all clones have similar reproductive characteristics with clones from conventional grafted. However, only KKM4 clone from immature zygotic embryo culture produced the shortest staminode to style distance of 1.83 mm. This consequently influenced flower stability by reducing the efficiency of pollination by insects. It was found that this clone also has the highest number of flowers drop after anthesis (5 flowers) and lowest production of cherelle (5 cherelles). Further observation revealed that floral development from first bud visible (BBCH51) to flower anthesis (BBCH68) of all clones took around 31 days. These cocoa flowers which remained receptive soon after anthesis at 10 am (day-31) until the next day (day-32) suggesting 2 days’ period of receptivity. HIGHLIGHTS It is crucial to assess the presence of off-type characteristics in the reproductive organ structure such as the distance between staminode to style, period of reproductive cycle and stigmatic receptivity of cocoa clones regenerated from somatic embryogenesis The converging and parallel type of staminode to style distances are the ideal flower spatial arrangements for the optimal pollination in cocoa plant compared to splay type Only KKM4 clone propagated from immature zygotic embryo culture showed variation in the distance between staminode to style distance and this caused pollination failure by insect which then consequently caused minimum cherelle production All regenerated cocoa clones observed with typical period of the reproductive cycle and stigmatic receptivity GRAPHICAL ABSTRACT

scholarly journals Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos

2020 ◽  
Vol 92 (suppl 1) ◽  
Author(s):  
SAMANTA S. DE CAMPOS ◽  
JONNY E. SCHERWINSKI-PEREIRA ◽  
REGINA B. BERND ◽  
CLAUDIMAR S. FIOR ◽  
SERGIO F. SCHWAZ
Keyword(s):  
Somatic Embryogenesis ◽  
Zygotic Embryos ◽  
In Vitro Multiplication

scholarly journals In vitro culture from mature seeds of Passiflora species

2004 ◽  
Vol 61 (1) ◽  
pp. 108-113 ◽  
Author(s):  
Flavia Guzzo ◽  
Stefania Ceoldo ◽  
Filippo Andreetta ◽  
Marisa Levi
Keyword(s):  
In Vitro Culture ◽  
Fruit Juice ◽  
Zygotic Embryo ◽  
South American ◽  
Zygotic Embryo Culture ◽  
In The Wild ◽  
American Tropics ◽  
Mature Seeds ◽  
Continuous Source

The genus Passiflora comprises hundred species, mainly native of the South American tropics and rainforests, which are grouped into 21 subgenera. Some species are widely studied for their economic importance and are chiefly cultivated for production of fruit juice. To obtain a continuous source of material for a screening of secondary metabolites, zygotic embryo culture was attempted for 62 Passiflora species, starting from seeds mainly collected in the wild. Twenty nine of these species produced calli, which had very different growth rates. Plants were successfully regenerated from calli of 13 different species. For 25 of the responsive species this is the first report of in vitro culture.

scholarly journals Coconut Tissue Culture: The Indian Initiatives, Experiences and Achievements

CORD ◽  
2017 ◽  
Vol 33 (2) ◽  
pp. 11
Author(s):  
Anitha Karun
Keyword(s):  
Tissue Culture ◽  
Somatic Embryos ◽  
Culture Media ◽  
Zygotic Embryo ◽  
Shoot Meristem ◽  
Direct Organogenesis ◽  
Zygotic Embryos ◽  
Immature Inflorescence ◽  
High Yielding Varieties

Coconut is one of the principal crops of India cultivated in over 35 districts mainly in the southern states. The productivity of the crop is declining in many of the traditionally cultivated regions owing to ageing plantations as well as biotic and abiotic stresses. These plantations are to be replanted with high yielding varieties/hybrids for which adequate quantity of quality planting material is not available. Even though tissue culture research was initiated in many laboratories in the country, the work was eventually phased out in most of the laboratories for want of a repeatable protocol.  At ICAR-CPCRI, coconut tissue culture programs have been continuing for the past three decades. The attempts made include experimentation with different explants viz., immature inflorescence, plumular tissues, mature palm shoot meristem, ovary and anthers and different culture media supplemented with varying levels and types of hormones. Some of the successful protocols developed at the Institute include coconut zygotic embryo culture for collection and exchange of germplasm, cryopreservation and retrieval of zygotic embryos and pollen and plantlet regeneration from plumular tissues. Even though ICAR-CPCRI has succeeded in obtaining plantlets via direct organogenesis from inflorescence explants, the absence of friable calli formation from explants, the low rate of somatic embryo formation, large number of cultures turning to abnormal shoot development, non conversion of somatic embryos into plantlets, and formation of abnormal somatic embryos remain the major bottlenecks. Gene expression studies are being currently undertaken to decipher the molecular basis of in vitro recalcitrance.

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