scholarly journals In vitro culture from mature seeds of Passiflora species

2004 ◽  
Vol 61 (1) ◽  
pp. 108-113 ◽  
Author(s):  
Flavia Guzzo ◽  
Stefania Ceoldo ◽  
Filippo Andreetta ◽  
Marisa Levi
Keyword(s):  
In Vitro Culture ◽  
Fruit Juice ◽  
Zygotic Embryo ◽  
South American ◽  
Zygotic Embryo Culture ◽  
In The Wild ◽  
American Tropics ◽  
Mature Seeds ◽  
Continuous Source

The genus Passiflora comprises hundred species, mainly native of the South American tropics and rainforests, which are grouped into 21 subgenera. Some species are widely studied for their economic importance and are chiefly cultivated for production of fruit juice. To obtain a continuous source of material for a screening of secondary metabolites, zygotic embryo culture was attempted for 62 Passiflora species, starting from seeds mainly collected in the wild. Twenty nine of these species produced calli, which had very different growth rates. Plants were successfully regenerated from calli of 13 different species. For 25 of the responsive species this is the first report of in vitro culture.

scholarly journals Kultur In Vitro Endosperma, Protokol yang Efisien untuk Mendapatkan Tanaman Triploid secara Langsung

2016 ◽  
Vol 6 (2) ◽  
pp. 107
Author(s):  
Lazarus Agus Sukamto
Keyword(s):  
In Vitro Culture ◽  
Zygotic Embryo ◽  
Direct Organogenesis ◽  
Ploidy Levels ◽  
Endosperm Culture ◽  
Zygotic Embryo Culture ◽  
Seedless Fruits ◽  
Long Time ◽  
Mature Endosperm

<p>In Vitro Culture of Endosperm: An efficient protocol to<br />propagate triploid plants directly. L. Agus Sukamto.<br />Triploid plants are very vigorous and beneficial since they<br />generally produce seedless fruits, bigger flowers, and produce<br />more volume of wood than the diploid counterparts.<br />The triploid plants can be produced by crossing diploid and<br />tetraploid plants, but this method is cumbersome and takes<br />a long time. In vitro culture of endosperm is an alternative<br />method to produce triploid plants directly. The success of<br />endosperm culture is dependent on many factors, such as<br />maturity of endosperm, presence of the zygotic embryo, culture<br />medium, growth regulators, browning, culture period,<br />an plant species. Generally, a mature endosperm needs an<br />initial association with an embryo to induce cell divisions,<br />while proliferation of an immature endosperms is not<br />dependent on the embryo. Endosperm of most parasitic<br />angiosperms shows direct organogenesis without callus<br />formation. Plants produced from endosperm culture are<br />generally triploid, although some plants possess different<br />ploidy levels.</p>

scholarly journals Enhanced Seed Germination and Seedling Growth of Tillandsia Eizii In Vitro

HortScience ◽  
2003 ◽  
Vol 38 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Kimberly A. Pickens ◽  
James M. Affolter ◽  
Hazel Y. Wetzstein ◽  
Jan H.D. Wolf
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
Seedling Growth ◽  
Culture Medium ◽  
Habitat Destruction ◽  
Light Conditions ◽  
Physiological Constraints ◽  
Large Numbers ◽  
In The Wild

Tillandsia eizii is an epiphytic bromeliad that due to over-collection, habitat destruction, and physiological constraints has declined to near threatened status. This species exhibits high mortality in the wild, and seed are characterized by low percentages of germination. As a means to conserve this species, in vitro culture protocols were developed to enhance seed germination and seedling growth. A sterilization protocol using 70% ethanol for 2 minutes followed by 2.6% NaOCl for 40 minutes disinfested seed and promoted seedling growth. Sucrose incorporated into the culture medium had no effect on germination or growth, while NAA inhibited growth, but not germination. Cultures maintained under a 16-hour photoperiod at 22 °C exhibited greater growth than those grown at 30 °C. Seed that germinated in the dark remained etiolated and failed to develop even after transfer to light conditions. Plants grown in vitro were successfully acclimatized and transferred to the greenhouse. Over 86% survival and rapid growth were obtained with either an all-pine-bark medium, or a mixture of 2 redwood bark: 2 fir bark: 2 potting mix: 1 perlite. This demonstrated that in vitro culture of seed may be used to rapidly produce large numbers of T. eizii, and thus can be used for the conservation and reintroduction of this species.

scholarly journals Formation and survival of in vitro protocormos of Cerrado Orchid of Goiás

2020 ◽  
Vol 13 (6) ◽  
pp. 1
Author(s):  
D. C. S. Lemos ◽  
G. R. Sousa ◽  
A. D. V. Souza ◽  
J. B. A. França ◽  
M. R. Rosa ◽  
...  
Keyword(s):  
Bacterial Contamination ◽  
Sodium Hypochlorite Solution ◽  
Ms Medium ◽  
In Vitro Development ◽  
Federal Institute ◽  
In The Wild ◽  
Vitro Development ◽  
Mature Seeds ◽  
Pot Plants

The Cerrado has wide plant diversity, including orchids. These plants need symbiosis with fungi for their germination to occur in the wild. In vitro or asbiotic cultivation is an alternative for the production of seedlings of this species. This diversity of orchids makes it much appreciated as pot plants, landscaping, with high commercial value. The objective of this work was to carry out the study for the development of a protocol for the germination and in vitro development of orchids. The experiment was carried out at the LABIOTEC Biotechnology Laboratory of the Federal Institute Goiano Campus Urutaí-GO. Catasetum sp native capsules from Goiás State, containing mature seeds were collected and disinfected in 50% sodium hypochlorite solution for 30 minutes and in laminar flow were washed four times in distilled and autoclaved water. They were then seeded in vitro in 258 mL glass vials containing 30 mL of MS medium enriched with vitamins and 0.0 Tryptone; 1.0; 2.0 and 3.0 g L-1. The pH of the medium was adjusted to 5.2 ± 0.1. The average time for germination and development of the protocorms varied from 3 to 7 days after sowing for the species under study. The dosage of 2.0 g L-1 is efficient for the development and maintenance of in vitro Catasetum protocorms up to 333 days. There was no development of shoot of Catasetum seedlings in any of the evaluated treatments. It was observed that the 3.0 g L-1 Tryptone dosage presented higher rates of fungal and bacterial contamination and oxidation

scholarly journals Cultivo in vitro de embrião zigótico de baru influenciado por tipos de vedações e concentrações de sacarose / In vitro culture of zygotic embryo of baru as affected by sealing types and sucrose concentrations

2021 ◽  
Vol 7 (4) ◽  
pp. 42390-42408
Author(s):  
Gabriel Garcia Barbosa ◽  
Victória Maria Ingre Targa ◽  
Wagner Campos Otoni ◽  
Josimara Nolasco Rondon ◽  
Francilina Araújo Costa
Keyword(s):  
In Vitro Culture ◽  
Zygotic Embryo

scholarly journals Standardization of zygotic embryo culture from Nerium oleander L. and comparative analysis of biosynthesized cardiac glycosides within in vitro and acclimatized plants

2021 ◽  
pp. 204-215
Author(s):  
Renu Nimoriya ◽  
Yatendra Singh ◽  
Sumit Kumar Singh ◽  
Pankaj Singh ◽  
Amar Jeet ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Cardiac Glycoside ◽  
Cardiac Glycosides ◽  
Zygotic Embryo ◽  
Germination Percentage ◽  
Zygotic Embryos ◽  
In Vitro Multiplication ◽  
Zygotic Embryo Culture ◽  
In Vitro Plantlets

The primary result of our experiment revealed that the germination percentage of N. oleander mature seeds is only 30%. From this observation, the concept of protocol standardization for zygotic embryo culture of this plant was originated. Zygotic embryo culture was proved an efficient in vitro multiplication system of N. oleander. The maximum germination percentage (96%) of zygotic embryos was observed on ¼ MS medium with 15 gm/L sucrose, whereas the best growth medium was optimized as ½ B5 with same sucrose concentration. The second part of this study was aimed to find out the cardiac glycoside accumulation pattern in both in vitro and acclimatized plants. For this purpose, one-month-old in vitro plantlets and acclimatized plants were subjected to LC-MS analysis and 09 cardiac glycosides were detected and quantified in both the systems. Most of the cardiac glycosides including odoroside A (32.71 mg/gm DW), odoroside H (4.69 mg/gm DW) and oleandrin (0.52 mg/gm DW) were found to be accumulated at maximum level within in vitro plantlets. CG 840b (1.89 mg/gm DW) is the only cardiac glycoside, which was maximally accumulated in acclimatized plants. From this study, it can be concluded that, zygotic embryo culture is a better choice for in vitro multiplication of N. oleander when compared to matured seeds and in vitro grown plantlets of this species favor cardiac glycosides biosynthesis in comparison to acclimatized plants. Therefore, all future research on the enrichment of cardiac glycosides from this plant may be conducted on zygotic embryos derived in vitro grown plantlets or cultures.

Zygotic embryo culture is an efficient way to optimize in vitro growth in Panax ginseng

2021 ◽  
Vol 167 ◽  
pp. 113497
Author(s):  
Jung-Woo Lee ◽  
Gyung-Ran Do ◽  
Ic-Hyun Jo ◽  
Chi-Eun Hong ◽  
Kyung-Hwan Bang ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
In Vitro Growth ◽  
Zygotic Embryo Culture

Arachis hypogaea Plant Recovery Through in Vitro Culture of Peg Tips1

1995 ◽  
Vol 22 (2) ◽  
pp. 129-135 ◽  
Author(s):  
Q. L. Feng ◽  
H. T. Stalker ◽  
H. E. Pattee ◽  
T. G. Isleib
Keyword(s):  
In Vitro Culture ◽  
Embryo Development ◽  
Arachis Hypogaea ◽  
Reproductive Traits ◽  
Root Production ◽  
In Vitro Techniques ◽  
Arachis Hypogaea L ◽  
Vitro Development ◽  
Mature Seeds

Abstract In vitro culture of embryos in Arachis is necessary to recover interspecific hybrids which otherwise abort soon after fertilization. The objective of this research was to develop in vitro techniques to promote proembryo development so that plants can be recovered. Aerial peg tips consisting of embryos, ovules, and peg meristem of Arachis hypogaea L. cv. NC 6, were collected 7, 10, and 14 d after self-pollination. Peg tips were cultured in the dark on combined MS and B5 media with NAA, GA3 and 6-BAP for 90 d. The effects of plant growth regulators on in vitro reproductive traits, including peg elongation, callus and root production, pod formation, ovule and embryo development were variable. Results indicated that 10-d-old peg tips, which contained eight-celled proembryos, had more embryo development and pod formation than 7- and 14-d-old peg tips. Medium with 4 mg L-1 NAA and 0.5 mg L-1 6-BAP suppressed in vitro development of pods, ovules and embryos and induced large amounts of callus. Media with lower concentrations of NAA, GA3, and 6-BAP caused development of more and larger pods and ovules. The development of young embryos from proembryos was observed and mature seeds were obtained by an in vitro one-step process. Peanut plants were obtained both from in vitro-recovered embryos and from mature seeds.

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