Detection of radiation treatment of spices using Electron Spin Resonance Spectroscopy Technique

Use of ionising radiation for commercial sterilisation to increase the hygienic quality of spices is increasing worldwide. Among several detection methods, Electron Spin Resonance (ESR) spectroscopy is one of the reliable techniques for irradiated foods containing some hard and dried parts. Spices are therefore candidates of such a method and radiation treated spices can be detected. The purpose of the present work was to identify the radiation treatment of spices like cinnamon, cardamom, ginger and rosemary using the sensitive technique of ESR spectroscopy. The spice samples were irradiated to 5, 10, 15 and 20 kilogray (kGy) or unirradiated and the samples were dried in vacuum oven and were placed into quartztubes for ESR measurement. On measurements, the unirradiated samples were found to show the ESR-signal at g-value of 2.004 that is typical in case of plant foods containing cellulose. Whereas, the irradiated samples showed a formation of a new paramagnetic structure that appeared in the form of a triplet-signal, attributed to radiation-induced cellulose radical. The clear difference between the nature of ESR signals in case of unirradiated and irradiated samples provided the evidence of the radiation treatment of spices samples. It is therefore concluded that radiation treatment of spices can be identified using sensitive ESR spectroscopy during a storage period of three to ten months.

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Radiation Dose Evaluation of Colorless Topaz by TL and ESR Spectroscopy

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.866.267 ◽

2017 ◽

Vol 866 ◽

pp. 267-271

Author(s):

Nisakorn Nuamsrinuan ◽

Weeranuch Kaewwiset ◽

Pichet Limsuwan ◽

Kittisakchai Naemchanthara

Keyword(s):

Electron Spin Resonance ◽

Radiation Dose ◽

Electron Spin ◽

Esr Spectroscopy ◽

Dose Range ◽

Percentage Error ◽

Dose Evaluation ◽

Routine Application ◽

Spin Resonance ◽

G Value

In the present work, colorless topaz was designed and developed a dosimeter for routine application. The samples were irradiated with gamma from 60Co at dose range 50 - 400 Gy and investigated by thermoluminescence (TL) and electron spin resonance (ESR). The TL grow curve of topaz were revealed two trapping levels at temperature 150 °C and 225 °C. The ESR signals of topaz shown that the g value at 1.966 and 2.012 due to Ti3+ and (AlO4)0 center, respectively. The intensities of TL and ESR were increased with increasing dose. The percentage error of dose from TL and ESR signal were 8 and 3, respectively. These experiment indicated that the colorless topaz could be used as a dosimeter.

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High Field-High Frequency Electron Spin Resonance Spectroscopy (Multi-Frequency ESR Spectroscopy)

Journal of the Spectroscopical Society of Japan ◽

10.5111/bunkou.55.369 ◽

2006 ◽

Vol 55 (6) ◽

pp. 369-378

Author(s):

Tatsuhisa KATO

Keyword(s):

Electron Spin Resonance ◽

Electron Spin ◽

High Frequency ◽

High Field ◽

Esr Spectroscopy ◽

Electron Spin Resonance Spectroscopy ◽

Resonance Spectroscopy ◽

Spin Resonance

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Electron Spin Resonance Spectroscopy of Serum Albumin: A Novel New Test for Cancer Diagnosis and Monitoring

Clinical Chemistry ◽

10.1373/clinchem.2006.073148 ◽

2006 ◽

Vol 52 (11) ◽

pp. 2129-2134 ◽

Cited By ~ 28

Author(s):

Steven C Kazmierczak ◽

Andrey Gurachevsky ◽

Gert Matthes ◽

Vladimir Muravsky

Keyword(s):

Electron Spin Resonance ◽

Stearic Acid ◽

Serum Albumin ◽

Cancer Patients ◽

Electron Spin ◽

Spin Probe ◽

Esr Spectroscopy ◽

Resonance Spectroscopy ◽

Esr Spectrum ◽

Spin Resonance

Abstract Background: Proteins released by tumor cells can bind to serum albumin, leading to structural and functional modifications. We used electron spin resonance (ESR) spectroscopy to measure these changes in serum albumin and evaluate their utility for the diagnosis and monitoring of cancer. Methods: We used an ESR spectrometer and 16-doxyl stearic acid as spin probe to measure conformational changes in albumin in blood samples from a population of healthy donors and volunteers (n = 349), patients with a wide variety of hematologic and nonhematologic malignancy (n = 135), and patients with chronic diseases such as gastrointestinal and pulmonary disease, diabetes, and cirrhosis (n = 91). We added differing amounts of 16-doxyl stearic acid spin probe in ethanol to 50 μL of serum from each patient to create 3 different aliquots that differed in concentration of spin probe and ethanol, then incubated the aliquots for 10 min at 37 °C with continuous shaking. We measured the ESR spectra of each aliquot in triplicate and used proprietary software (MedInnovation GmbH) to evaluate the ESR spectrum for differences between cancer patients and the other groups. Results: The diagnostic sensitivity and specificity of this test were 87.4% and 95.7%, respectively, for differentiating healthy individuals from cancer patients and 87.4%, and 85.7% for differentiating cancer patients from chronic disease patients. Serial evaluation of albumin conformation changes in several patients followed during the course of their disease showed excellent agreement between the magnitude of abnormality in the ESR spectrum of albumin and clinical and pathologic estimates of disease severity. Conclusions: ESR spectroscopy of serum albumin is a sensitive and noninvasive technique that clearly demonstrates diagnostic utility in patients with cancer. This test also enables monitoring of the disease course through use of serial measurements.

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