Alternative treatments diminish oviposition and viability of Leucoptera coffeella (Lepidoptera: Lyonetiidae) eggs and larvae on coffee plants

Transformation of coffee (Coffea Arabica L. cv. Catimor) with the cry1ac gene by biolistic, without the use of markers

Brazilian Journal of Biology ◽

10.1590/s1519-69842010000200022 ◽

2010 ◽

Vol 70 (2) ◽

pp. 387-393 ◽

Cited By ~ 6

Author(s):

Z De Guglielmo-Cróquer ◽

I. Altosaar ◽

M. Zaidi ◽

A. Menéndez-Yuffá

Keyword(s):

Reporter Genes ◽

Leaf Miner ◽

Selection Marker ◽

Marker Genes ◽

Cry1ac Gene ◽

Reverse Transcription Pcr ◽

Gus Reporter ◽

Leucoptera Coffeella ◽

Coffee Plants ◽

Coffea Arabica L

The transformation of coffee plantlets with the cry1ac gene of Bacillus thuringiensis was achieved by biolistic using either the whole pUBC plasmid or only the ubi-cry1ac-nos genetic cassette. The cry1ac gene was inserted into coffee plants in order to confer resistance to the leaf miner Leucoptera coffeella, an insect responsible for considerable losses in coffee crops. Bearing in mind that the genetic cassettes used for this study lack reporter genes and/or selection marker genes, the parameters for the transformation procedure by biolistic were previously standardised with a plasmid carrying the gus reporter gene. The presence of the cry1ac gene in young plantlet tissues was determined by PCR, Southern blot and reverse transcription-PCR. Our results show that the obtainment of viable coffee plantlets, transformed by bombardment with the cry1ac gene and without selection markers nor reporter genes, is feasible.

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Toxicity of organic farming‑compatible products to the coffee leaf miner

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2013000300001 ◽

2013 ◽

Vol 48 (3) ◽

pp. 241-248 ◽

Cited By ~ 1

Author(s):

Madelaine Venzon ◽

Rodrigo Ferreira Krüger ◽

Alberto Soto ◽

Edmar de Souza Tuelher ◽

Italo Santos Bonomo ◽

...

Keyword(s):

Organic Farming ◽

Field Trial ◽

Bordeaux Mixture ◽

Leaf Miner ◽

Population Decrease ◽

Lime Sulphur ◽

Leucoptera Coffeella ◽

Coffee Leaf Miner ◽

Ovicidal Effects ◽

Eggs And Larvae

The objective of this work was to evaluate the toxicity of organic farming‑compatible products to the coffee leaf miner Leucoptera coffeella. Lime sulphur, enriched Bordeaux mixture (Viça Café Plus), and the "supermagro" biofertilizer were first tested in laboratory. The most promising product was tested afterwards under field conditions. In laboratory, different concentrations of each product were applied on L. coffeella eggs and on infested coffee‑mined leaves. Only lime sulphur had ovicidal effects at an acceptable concentration (1.6%) for field applications, but no significant effect on larvae mortality was found. Enriched Bordeaux mixture and the "supermagro" biofertilizer had no effect on L. coffeella eggs and larvae. In the field trial, biweekly or monthly sprayings of lime sulphur at different concentrations caused population decrease after 30 days; however, this effect was not significant after 60 or 90 days.

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APPLICATIONS OF COPPER-BASED FUNGICIDES AND INFESTATIONS OF Leucoptera coffeella (Guérin-Mèneville & Perrottet) IN COFFEE PLANTS

Coffee Science ◽

10.25186/cs.v14i1.1504 ◽

2019 ◽

Vol 14 (1) ◽

pp. 123

Author(s):

Paulo Henrique Siqueira Sabino ◽

Gian Otavio Alves Da Silva ◽

Adriano Bortolotti Da Silva ◽

Geraldo Andrade Carvalho

Keyword(s):

Leaf Surface ◽

Leaf Miner ◽

Coffee Plantation ◽

Leaf Surfaces ◽

Number Of Leaves ◽

Leucoptera Coffeella ◽

Coffee Plants ◽

Wax Content ◽

Different Sources

The present study aimed to evaluate the effects of applying fungicides with different sources of copper and of the number of applications on the occurrence of <em>Leucoptera coffeella </em>(Guérin-Menéville & Perrottet, 1842) (Lepidoptera: Lyonetiidae) and on the wax layer on leaves in a coffee plantation. Four applications of fungicides were carried out and the effects on the number of leaves mined by the insect and on the wax content on the leaf surface were evaluated. The copper-based fungicides increased the number of leaves mined by the leaf-miner and reduced the wax content on the coffee leaf surfaces in both periods studied.

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