scholarly journals Transformation of coffee (Coffea Arabica L. cv. Catimor) with the cry1ac gene by biolistic, without the use of markers

2010 ◽  
Vol 70 (2) ◽  
pp. 387-393 ◽  
Author(s):  
Z De Guglielmo-Cróquer ◽  
I. Altosaar ◽  
M. Zaidi ◽  
A. Menéndez-Yuffá
Keyword(s):  
Reporter Genes ◽  
Leaf Miner ◽  
Selection Marker ◽  
Marker Genes ◽  
Cry1ac Gene ◽  
Reverse Transcription Pcr ◽  
Gus Reporter ◽  
Leucoptera Coffeella ◽  
Coffee Plants ◽  
Coffea Arabica L

The transformation of coffee plantlets with the cry1ac gene of Bacillus thuringiensis was achieved by biolistic using either the whole pUBC plasmid or only the ubi-cry1ac-nos genetic cassette. The cry1ac gene was inserted into coffee plants in order to confer resistance to the leaf miner Leucoptera coffeella, an insect responsible for considerable losses in coffee crops. Bearing in mind that the genetic cassettes used for this study lack reporter genes and/or selection marker genes, the parameters for the transformation procedure by biolistic were previously standardised with a plasmid carrying the gus reporter gene. The presence of the cry1ac gene in young plantlet tissues was determined by PCR, Southern blot and reverse transcription-PCR. Our results show that the obtainment of viable coffee plantlets, transformed by bombardment with the cry1ac gene and without selection markers nor reporter genes, is feasible.

scholarly journals APPLICATIONS OF COPPER-BASED FUNGICIDES AND INFESTATIONS OF Leucoptera coffeella (Guérin-Mèneville & Perrottet) IN COFFEE PLANTS

2019 ◽  
Vol 14 (1) ◽  
pp. 123
Author(s):  
Paulo Henrique Siqueira Sabino ◽  
Gian Otavio Alves Da Silva ◽  
Adriano Bortolotti Da Silva ◽  
Geraldo Andrade Carvalho
Keyword(s):  
Leaf Surface ◽  
Leaf Miner ◽  
Coffee Plantation ◽  
Leaf Surfaces ◽  
Number Of Leaves ◽  
Leucoptera Coffeella ◽  
Coffee Plants ◽  
Wax Content ◽  
Different Sources

The present study aimed to evaluate the effects of applying fungicides with different sources of copper and of the number of applications on the occurrence of <em>Leucoptera coffeella </em>(Guérin-Menéville &amp; Perrottet, 1842) (Lepidoptera: Lyonetiidae) and on the wax layer on leaves in a coffee plantation. Four applications of fungicides were carried out and the effects on the number of leaves mined by the insect and on the wax content on the leaf surface were evaluated. The copper-based fungicides increased the number of leaves mined by the leaf-miner and reduced the wax content on the coffee leaf surfaces in both periods studied.

scholarly journals EFFECT OF IRRIGATION ON ROOT DEVELOPMENT OF COFFEE PLANTS

Irriga ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 378-391
Author(s):  
Marcelo Rossi Vicente ◽  
Everardo Chartuni Mantovani ◽  
André Luís Teixeira Fernandes ◽  
Júlio Cesar Lima Neves ◽  
Edmilson Marques Figueredo ◽  
...  
Keyword(s):  
Root System ◽  
Root Development ◽  
Coffea Arabica ◽  
Crop Yields ◽  
Root Length Density ◽  
Block Design ◽  
Minas Gerais ◽  
Root Weight ◽  
Coffee Plants ◽  
Coffea Arabica L

EFFECT OF IRRIGATION ON ROOT DEVELOPMENT OF COFFEE PLANTS     MARCELO ROSSI VICENTE1; EVERARDO CHARTUNI MANTOVANI2; ANDRÉ LUÍS TEIXEIRA FERNANDES3; JÚLIO CÉSAR LIMA NEVES4; EDMILSON MARQUES FIGUEREDO5 E FÁBIO TEIXEIRA DELAZARI6   1 Instituto Federal do Norte de Minas Gerais, Campus Salinas, Fazenda Varginha, Rodovia Salinas – Taiobeiras, Km 2, 39560-00, Salinas, Minas Gerais, Brasil, [email protected] 2 Departamento de Engenharia Agrícola e Ambiental, Universidade Federal de Viçosa, Campus Universitário, Viçosa, 36570.900, Minas Gerais, Brasil, [email protected] 3 Universidade de Uberaba, Av. Nenê Sabino, n° 1801, 38055-500, Uberaba, Minas Gerais, Brasil, [email protected] 4 Departamento de Solos, Universidade Federal de Viçosa, Campus Universitário, Viçosa, 36570-900, Minas Gerais, Brasil, [email protected] 5 Bahia Farm Show, Av. Ahylon Macedo, n° 919, 97810-035, Barreiras, Bahia, Brasil, [email protected] 6 Departamento de Fitotecnia, Universidade Federal de Viçosa, Campus Universitário, Viçosa, 36570-900, Minas Gerais, Brasil, [email protected]     1 ABSTRACT   Increasing the development and deepening of the root system in coffee crops ensures higher water and nutrient uptakes as a result of improved soil utilization, ultimately leading to greater crop yields and longevity. The aim of this study was to evaluate the effect of irrigation levels on the root system of drip-irrigated coffee plants in western Bahia State (BA), Brazil. The experiment was carried out on Café do Rio Branco farm, located in Barreiras - BA, using adult plants (approximately 3.5 years old) of coffee variety Catuaí Vermelho IAC 144. The experiment was set up as a randomized block design with three treatments corresponding to the irrigation depths of 75, 100 and 150% as determined using Irriplus software. After the fourth harvest, the coffee root system was assessed to determine root length density (RLD) and root weight density (RWD) in different sampled layers. A greater concentration of roots (RLD and RWD) was observed in the surface layer (0-20 cm) and under the lateral line (at 30 and 70 cm from the orthotropic branch). The irrigation depth of 75% provided the highest concentration of roots (RLD and RWD) in the 0-10 cm layer.   Keywords: Drip irrigation, Coffea arabica L, root system.     VICENTE, M.R.; MANTOVANI, E.C.; FERNANDES, A.L.T.; NEVES, J.C.L.; FIGUEREDO, E.M.; DELAZARI, F.T EFEITO DA IRRIGAÇÃO NO DESENVOLVIMENTO RADICULAR DO CAFEEIRO     2 RESUMO   Um maior desenvolvimento e aprofundamento do sistema radicular garante ao cafezal um aumento da absorção de água e nutrientes devido a maior exploração do solo, com isto maior produtividade e longevidade da lavoura. O objetivo deste trabalho foi avaliar os efeitos de diferentes lâminas de irrigação sobre o sistema radicular do cafeeiro irrigado por gotejamento na região Oeste da Bahia. Realizou-se o trabalho na fazenda Café do Rio Branco, localizada em Barreiras - BA em cafeeiros adultos, aproximadamente 3,5 anos de idade, da variedade Catuaí Vermelho IAC 144. O experimento ocorreu no delineamento em blocos casualizados, composto de 3 tratamentos, correspondentes à 75, 100 e 150% da lâmina de irrigação determinada pelo software Irriplus. Após a quarta safra, procedeu-se às avaliações do sistema radicular do cafeeiro, onde foi determinada a densidade de comprimento radicular - DCR e a densidade radicular – DR em diferentes camadas amostradas. Observou-se maior concentração de raízes, DCR e DR, na camada superficial (0-20 cm) e sob a linha lateral (30 e 70 cm de distância do ramo ortotrópico). A lâmina de irrigação correspondente a 75% proporcionou maior concentração de raízes (DCR e DR) na camada de 0 a 10 cm.   Palavras - chaves: Irrigação localizada, Coffea arábica L, sistema radicular

scholarly journals Effect of leaf Water potential on cold tolerance of Coffea arabica L.

2002 ◽  
Vol 45 (4) ◽  
pp. 439-443 ◽  
Author(s):  
Lázara Pereira Campos Caramori ◽  
Paulo Henrique Caramori ◽  
João Manetti Filho
Keyword(s):  
Water Potential ◽  
Leaf Water Potential ◽  
Coffea Arabica ◽  
Experimental Period ◽  
Leaf Water ◽  
Regional Variability ◽  
Wide Range ◽  
Coffee Plants ◽  
Coffea Arabica L ◽  
Severe Leaf

Young coffee plants from cultivar Mundo Novo of Coffea arabica were grown without irrigation for 32 consecutive days, to evaluate the effect of leaf water potential on damage caused by low temperatures, under controlled conditions. A wide range of leaf water potentials were evaluated, from - 0.45MPa (wet soil) at the beginning of the experimental period, to - 4.8MPa (severe leaf wilting) at the end. Results showed that under moderate water stress, there was a higher frequency of undamaged plants and lower frequency of severely damaged plants. These results help explain part of the regional variability observed after a frost and stress the importance of new studies associating cold and drought tolerance in coffee.

A chemical-inducible Cre-LoxP system allows for elimination of selection marker genes in transgenic apricot

2012 ◽  
Vol 110 (3) ◽  
pp. 337-346 ◽  
Author(s):  
César Petri ◽  
Sonia López-Noguera ◽  
Hong Wang ◽  
Carlos García-Almodóvar ◽  
Nuria Alburquerque ◽  
...  
Keyword(s):  
Selection Marker ◽  
Marker Genes

Use of a counterselectable transposon to create markerless knockouts from a 18,432-clone ordered M. bovis BCG mutant resource

2019 ◽  
Author(s):  
Katlyn Borgers ◽  
Kristof Vandewalle ◽  
Annelies Van Hecke ◽  
Gitte Michielsen ◽  
Evelyn Plets ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Vaccine Development ◽  
Cost Effective ◽  
Essential Genes ◽  
Selection Marker ◽  
Marker Genes ◽  
Gene Effects ◽  
Genome Wide ◽  
Effective Manner ◽  
Slow Growing

AbstractMutant resources are essential to improve our understanding of the biology of slow-growing mycobacteria, which include the causative agents of tuberculosis in various species, including humans. The generation of deletion mutants in slow-growing mycobacteria in a gene-by-gene approach in order to make genome-wide ordered mutant resources is still a laborious and costly approach; despite the recent development of improved methods. On the other hand, transposon mutagenesis in combination with Cartesian Pooling-Coordinate Sequencing allows the creation of large archived Mycobacterium transposon insertion libraries. However, such mutants contain selection marker genes with a risk of polar gene effects, which is undesired both for research and for use of these mutants as live attenuated vaccines. In this paper, a derivative of the Himar1 transposon is described, which allows the generation of clean, markerless knockouts from archived transposon libraries. By incorporating FRT sites for FlpE/FRT-mediated recombination and I-SceI sites for ISceIM-based transposon removal, we enable two thoroughly experimentally validated possibilities to create unmarked mutants from such marked transposon mutants. The FRT approach is highly efficient but leaves an FRT scar in the genome, whereas the I-SceI mediated approach can create mutants without any heterologous DNA in the genome. The combined use of CP-CSeq and this optimized transposon was applied in the BCG Danish 1331 vaccine strain (WHO reference 07/270), creating the largest ordered, characterized resource of mutants in a member of the M. tb complex (18,432 clones, mutating 83% of the non-essential M. tb homologues), from which clean knockouts can be generated.ImportanceWhile speeding up research for many fields of biology (e.g. yeast, plant, and C. elegans), genome-wide ordered mutant collections are still elusive in mycobacterial research. We developed methods to generate such resources in a time- and cost-effective manner, and developed a newly engineered transposon from which unmarked mutants can be efficiently generated. Our library in the WHO reference vaccine strain of M. bovis BCG Danish targets 83% of all non-essential genes and was made publicly available via the BCCM/ITM Mycobacteria Collection. This resource will speed up Mycobacterium research (e.g. drug resistance research, vaccine development) and paves the way to similar genome-wide mutant collections in other strains of the M. tb complex. The stretch to a full collection of mutants in all non-essential genes is now much shorter, with just 17% remaining genes to be targeted using gene-by-gene approaches, for which highly effective methods have recently also been described.

scholarly journals Insecticide use and organophosphate resistance in the coffee leaf miner Leucoptera coffeella (Lepidoptera: Lyonetiidae)

2002 ◽  
Vol 92 (3) ◽  
pp. 203-212 ◽  
Author(s):  
D.B. Fragoso ◽  
R.N.C. Guedes ◽  
M.C. Picanço ◽  
L. Zambolim
Keyword(s):  
Insecticide Resistance ◽  
Methyl Parathion ◽  
Leaf Miner ◽  
Organophosphate Resistance ◽  
Standard Population ◽  
Resistant Population ◽  
Insect Populations ◽  
Leucoptera Coffeella ◽  
Coffee Leaf Miner ◽  
Brazilian Populations

AbstractIncreasing rates of insecticide use against the coffee leaf miner Leucoptera coffeella (Guérin-Méneville) and field reports on insecticide resistance led to an investigation of the possible occurrence of resistance of this species to some of the oldest insecticides used against it in Brazil: chlorpyrifos, disulfoton, ethion and methyl parathion. Insect populations were collected from ten sites in the state of Minas Gerais, Brazil and these populations were subjected to discriminating concentrations established from insecticide LC99s estimated for a susceptible standard population. Eight of the field-collected populations showed resistance to disulfoton, five showed resistance to ethion, four showed resistance to methyl parathion, and one showed resistance to chlorpyrifos. The frequency of resistant individuals in each population ranged from 10 to 93% for disulfoton, 53 to 75% for ethion, 23 to 76% for methyl parathion, and the frequency of resistant individuals in the chlorpyrifos resistant population was 35%. A higher frequency of individuals resistant to chlorpyrifos, disulfoton and ethion was associated with greater use of insecticides, especially other organophosphates. This finding suggests that cross-selection, mainly between organophosphates, played a major role in the evolution of insecticide resistance in Brazilian populations of L. coffeella. Results from insecticide bioassays with synergists (diethyl maleate, piperonyl butoxide and triphenyl phosphate) suggested that cytochrome P450-dependent monooxygenases may play a major role in resistance with minor involvement of esterases and glutathione S-transferases.

scholarly journals Development and optimization of Lysis gene E as a counter-selection marker with high selection stringency

2021 ◽  
Author(s):  
Wei Chen ◽  
Ruyi Chen ◽  
Ling He ◽  
Xiaotong Wu
Keyword(s):  
Regulatory System ◽  
Selection Marker ◽  
Marker Genes ◽  
Selection System ◽  
Gram Negative ◽  
E Coli ◽  
Lysis Gene ◽  
Counter Selection ◽  
Practical Research ◽  
High Selection

Seamless modification of bacteria chromosome is widely performed both in theoretical and in practical research, for this purpose, excellent counter-selection marker genes with high selection stringency are needed. Lysis gene E from bacteriophage PhiX174 was developed and optimized as a counter-selection marker in this paper. Lysis gene E was firstly constructed under the control of pL promoter. At 42 °C, Lysis gene E could effectively kill Escherichia coli. Seamless modification using E as a counter-selection marker also successfully conducted. It also works in another Gram-negative strain Serratia marcescens under the control of Arac/PBAD regulatory system. Through combining lysis gene E and kil, the selection stringency frequency of pL-kil-sd-E cassette in E. coli arrived at 4.9×10−8 and 3.2×10−8 at two test loci, which is very close to the best counter-selection system, inducible toxins system. Under the control of Arac/PBAD, selection stringency of PBAD-kil-sd-E in S. marcescens arrived at the level of 10−7 at four test loci. By introducing araC gene harboring plasmid pKDsg-ack, 5- to 18- fold improvement of selection stringency was observed at these loci, and a surprising low selection stringency frequency 4.9×10−9 was obtained at marR-1 locus, the lowest selection stringency frequency for counter-selection reported so far. Similarly, at araB locus of E. coli selection stringency frequency of PBAD-kil-sd-E was improved to 3×10−9 after introducing plasmid pKDsg-ack. In conclusion, we have developed and optimized a newly universal counter-selection marker based on lysis gene E. The best selection stringency of this new marker exceeds the inducible toxins system several fold.

scholarly journals Improvement Generative Growth of Coffea arabica L. Using Plant Growth Regulators and Pruning

2021 ◽  
Vol 226 ◽  
pp. 00003
Author(s):  
Ade Astri Muliasari ◽  
Ratih Kemala Dewi ◽  
Hidayati Fatchur Rochmah ◽  
Andoniana Rakoto Malala ◽  
Praptiningsih Gamawati Adinurani
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Plant Growth Regulator ◽  
Production Potential ◽  
Affected Plant ◽  
Main Plot ◽  
Coffee Plants ◽  
Coffea Arabica L ◽  
Number Of Branches ◽  
Plot Design

Pruning coffee plants is intended to stimulate generative growth that increases productivity to reach optimal. Plant Growth Regulator (PGR) makes it possible to exploit the production potential of plants. Pruning management, combined with the PGR in coffee plants, is expected to improve flowering and berry growth. The research was conducted for 7 mo from October 2017 to April 2018 at Gunung Gede, Bogor, West Java, Indonesia. A split–plot design with two treatment factors was used in this research. Pruning was placed as the main plot consisting of two levels, namely without (P0) and with pruning (P1). The PGR application was placed as a subplot, consisting of three dosages, namely without PGR (Z0), and concentrations of 0.3 mL L–1 (Z1) and 0.4 mL L–1 (Z2). The results showed, the pruning treatment significantly influenced microclimate, number of branches, B0, B1 and B2, number of berry sets, and berries. Pruning treatment had a very significant effect on microclimate, plant height, number of branches, number of coffee fruits, yields crops–1, and productivity. Interaction both significantly affected plant height and B0. The best dosage had not yet been found.

scholarly journals Reproductive capacity of Mirax insularis - Muesebeck (Hym: Braconidae) in larvae of the coffee leaf miner, Leucoptera coffeella- GuérinMéneville and Perrottet 1842 (Lep: Lyonetiidae).

1969 ◽  
Vol 96 (3-4) ◽  
pp. 221-234
Author(s):  
Marcela Daza ◽  
Fernando Gallardo
Keyword(s):  
Reproductive Potential ◽  
Reproductive Capacity ◽  
Leaf Miner ◽  
Experimental Unit ◽  
Artificial Rearing ◽  
Significant Difference ◽  
Rearing Conditions ◽  
Leucoptera Coffeella ◽  
Correlation Tests ◽  
Coffee Leaf Miner

The braconid Mirax insularis Muesebeck Is a koinoboint parasitoid of the coffee leafminer, Leucoptera coffeella Guérin-Méneville & Perrottet, ¡n Puerto Rico. However, their reproductive capacity under artificial rearing conditions of the leafminer is not known. The objective of this study was to determine its reproductive potential in larvae of 1st and 2nd instar of L. coffeella. Reproductive capacity tests showed significant differences (test t-Student, P < 0.05) in the preference of M. insularis for 2nd instar larvae with 7% of parasitization, whereas in 1st instar it gained only 5%. The average oviposition of M. insularis increases three folds (14 eggs per female) in a period of 48 hours on 2nd instar when compared to 1st instar. In the analysis of variance, the treatments that showed a significant difference (Tukey test, P < 0.05) were those with densities of 60 to 80 parasitoids per experimental unit on 2nd instar. Correlation tests find that there is a correlation (R = 0.453, P = 0.547) among the progeny of M. insularis produced when using larvae of the 1st instar. However, there is a positive correlation (R = 0.981, P = 0.019) when using 2nd instar larvae. The parasitization of M. insularis is higher when performing inoculations with 60 to 80 parasitoids on 2nd instar, producing a progeny of 365 and 480 parasitoids, respectively.

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