The effect of a direct-fed microbial (DFM) poduct containing a mixed culture of Lactobacillus casei and L. lactis on in vitro ruminal fermentation of barley-grain/barley-silage-based backgrounding and finishing diets and on growth performance and carcass characteristics of feedlot cattle was evaluated during backgrounding (84 d) and finishing (140 d) of 100 Hereford × Angus steers (initial body weight = 280 ± 15.5 kg). The inclusion rates of DFM in the in vitro study were 4, 8, 12 and 16 million colony forming units (CFU) of lactic acid bacteria (LAB) kg-1 DM of substrate. Total in vitro volatile fatty acids (VFA) production increased at 6 and 12 h of incubation (P < 0.01; linear response) when the backgrounding diet was supplemented with DFM. Dry matter digestibility and VFA production also increased (P < 0.05) during a 12-h fermentation of the finishing diet. Steers were randomly allocated to one of four dietary treatments that comprised feeding DFM tp provide 0 (control), 4 × 107, 8 × 107, or 12 × 107 CFU kg-1 diet DM. Average daily gain (ADG, kg) and feed efficiency (G:F; kg gain kg-1 DM consumed) of steers improved (P = 0.002 and 0.001, respectively) as a result of feeding DFM during the backgrounding period, but not during the finishing period. Saleable meat and rib eye area decreased (P = 0.038, linear; and P = 0.041, quadratic) with DFM supplementation. The results indicated that supplementing barley-grain/barley-silage-based feedlot cattle diets with 12 × 107 CFU of the mixed culture of lactobacilli used in these studies could improve ruminal fermentation, ADG and G:F in backgrounding feedlot steers. However, supplementation during the finishing period may not be warranted in terms of growth performance and carcass characteristics. Differences in the microbial ecology of the intestinal tract as a result of differences in diet composition may account for the varied response between the backgrounding and finishing periods.Key words: Beef cattle, direct-fed microbial, growth performance, Lactobacillus casei, Lactobacillus lactis, ruminal fermentation
Effect of age of suckler beef calves on stress indicators and growth performance in response to Burdizzo castration
