scholarly journals Effect of Thidiazuron, Benzylaminopurine and Naphthalene Acetic acid on In vitro propagation of Tuberose (Polianthes tuberosa L.) from shoot tip explants

2005 ◽  
Vol 6 (1) ◽  
Author(s):  
MJ Hutchinson ◽  
R Onamu ◽  
S Obukosia
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Shoot Tip ◽  
Naphthalene Acetic Acid ◽  
Shoot Tip Explants

scholarly journals In Vitro Shoot Proliferation and Root Induction of Shoot Tip Explants from Mature Male Plants of Casuarina cunninghamiana Miq.

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 797-800 ◽  
Author(s):  
Xiuli Shen ◽  
William S. Castle ◽  
Frederick G. Gmitter
Keyword(s):  
Acetic Acid ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Casuarina Cunninghamiana ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Proliferation

This study examined the effects of plant growth regulators, explant types, and their orientations on in vitro shoot proliferation of Casuarina cunninghamiana Miq. and also the subsequent rooting ability of shoots. Results showed that shoot proliferation occurred only in shoot tip explants cultured vertically on Murashige and Skoog (MS) medium supplemented with 2 or 4 μM thidiazuron (TDZ). Neither 6-benzylaminopurine alone nor in a combination with 1-naphthalene acetic acid (NAA) or gibberellic acid had any effect on shoot proliferation. TDZ at 4 μM resulted in the greatest percentage of axillary bud sprouting (70%) and mean number of sprouts per explant (2.3). Additionally, no shoot proliferation was observed from detipped or single-node explants or from horizontally placed shoot tip explants when cultured on the same TDZ-containing medium. The induced shoots produced adventitious roots on MS medium supplemented with 2.5, 5, or 10 μM indole-3-butyric acid (IBA), not with indole-3-acetic acid and NAA. Although the mean number of roots per explant was not significantly different between 2.5 and 5 μM IBA, the highest rooting percentage (68%) and mean length of roots per explant (0.7 cm) was achieved at 5 μM IBA. The current study provided preliminary information toward commercial in vitro propagation of Casuarina cunninghamiana male plants.

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey

2020 ◽  
Author(s):  
Nurşen Çördük ◽  
Cüneyt Aki
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Northwestern Turkey ◽  
Helen Of Troy ◽  
Endemic Medicinal Plant

Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regeneration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber.

In vitro propagation, genetic and phytochemical assessment of Thymus persicus — a medicinally important source of pentacyclic triterpenoids

Biologia ◽  
2014 ◽  
Vol 69 (5) ◽  
Author(s):  
Ziba Bakhtiar ◽  
Mohammad Mirjalili ◽  
Ali Sonboli ◽  
Mahdi Farimani ◽  
Mahdi Ayyari
Keyword(s):  
Acetic Acid ◽  
High Performance ◽  
In Vitro Propagation ◽  
Random Amplified Polymorphic Dna ◽  
Direct Organogenesis ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Pentacyclic Triterpenoids ◽  
Thymus Persicus

AbstractThymus persicus (Ronniger ex Rech. f.) Jalas is a valuable and endangered natural source of antitumor pentacyclic triterpenoids, i.e., betulinic acid, oleanolic acid and ursolic acid, which grows in northwest Iran. As the plant has a low propagation rate in nature, a suitable method for in vitro-propagation is needed. With the aim of identifying a suitable system for regenerating T. persicus via direct organogenesis, Murashige & Skoog (MS) medium supplemented with different plant growth regulators (PGRs) was tested. In vitro-grown shoot tips were exposed to the cytokinins 6-benzylaminopurine (BAP), kinetin (KN), and thidiazuron (TDZ), alone or in combination with the auxins 1-naphthalene-acetic acid (NAA), 2,4-dichlorophenoxyacetic (2,4-D), indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The highest shoot formation (7.1 ± 0.9) was obtained with a medium fortified with 8.9 μM BAP plus 2.7 μM NAA. Regenerated shoots were easily rooted on the different tested media, with the most abundant (16.6 ± 1.4) and strongest roots obtained on half-strength MS medium containing 2.5 μM IBA. The rooted plantlets were successfully acclimatized (76.6%) in a greenhouse before transference to natural conditions. Homogeneity and phytochemical productivity of the in vitro regenerated plantlets were confirmed by random amplified polymorphic DNA (RAPD) profiles and high performance liquid chromatography (HPLC), respectively.

scholarly journals PENGARUH EKSPLAN DAN ZPT TERHADAP PERTUMBUHAN nEPEnTHES ALBOmARGInATA SECARA In VITRO

2016 ◽  
Vol 12 (1) ◽  
pp. 103
Author(s):  
Lazarus Agus Sukamto
Keyword(s):  
Multiple Shoots ◽  
Carnivorous Plant ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Over Exploitation ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Shoot Tip Explants

Nepenthes albomarginata Lobb ex Lindl. is a carnivorous plant, distributes in several regions in Indonesia. The plant population decreases drastically because of over exploitation and ruining nature habitat. Plant propagation by nature and cutting are not enough to rehabilitation its population. In vitro culture of N. albomarginata was carried out using plantlets grown from the seeds in vitro. Plantlets were cut to became two part explants, consisted of shoot tip and under-shoot tip cuttings. These cutting explants were grown on Murashige & Skoog (MS) media with addition of plant growth regulators of 6-benzylaminopurine (BA), combined with or without-naphthalene acetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) at 1 mg/l. Shoot tip cuttings of N. albomarginata formed double multiple shoot 25,00% on control; formed triple multiple shoots 25,00% onBA 1 mg/l treatment; formed callus 37,50%, triple or quartet shoots 25,00% and rooted plantlets 25,00% on BA 1 mg/l + NAA 1 mg/l treatment. The under-shoot tip cuttings ofN. albomarginata formed double – triple shoots 25,00% and rooted plantlets 37,50% on control; formed double – triple shoots 25,00% and rooted plantlets 12,50% on BA 1 mg/ltreatment; formed callus 12,50%, double - pentacle shoots 37,50% and rooted plantlets 25,00% on BA 1 mg/l + NAA 1 mg/l treatment. 2,4-D 1 mg/l or its combined with BA 1mg/l treatment caused deadly shoot tip or under-shoot tip explants. The combination of BA 1 mg/l + NAA 1 mg/l was the best treatment for producing callus, multiple shootsand rooted plantlets of N. albomarginata.

scholarly journals IN VITRO PROPAGATION OF ALGERIAN IVY (HEDERA CANARIENSIS L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1123g-1124
Author(s):  
Karim H. Al-Juboory ◽  
David J. Williams
Keyword(s):  
Root Length ◽  
In Vitro Propagation ◽  
Inverse Relationship ◽  
Basal Medium ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Tip Explants ◽  
Strength Medium

Shoot tip explants of Algerian Ivy Heder a canariensis were cultured on MS basal medium supplemented with a combination of salt strength and NAA and IBA. More roots per explant developed on full salt strength medium combined with NAA. The most roots per explant were obtained with a combination of IBA and 1/4 MS salt. There was an inverse relationship between an increase in IBA or NAA concentration and root length and number. Shoots proliferated better on full MS salt combined with NAA and IBA. The highest level of NAA (40 uM) and 0.1 uM TDZ produced the most shoots and roots, the longest roots, the highest rooting percentage, the largest plants with the most leaves and the best callus quality per explant. The leaves from in vitro were cultured on MS medium with varying levels of Thidiazuron (TDZ) and NAA in the presence of light produced the highest number of roots.

scholarly journals Establishment and In Vitro Propagation of a Putative Variant of Periwinkle

2000 ◽  
Vol 5 (1) ◽  
pp. 15
Author(s):  
A. S. AI-Wasel
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Bud Breaking

Shoot multiplication of a putative variant of Catharanthus roseus (L.) G. Don, was achieved in vitro using shoot tips and nodal segments as explants. The addition of growth regulators to establishment medium stimulated bud breaking and shoot elongation. The maximum shoot multiplication (15.1 shoots/microshoot) and the longest shoots (7.0 cm) occurred on Murashige and Skoog medium (MS) containing 1.0 mg L-1 of N6-Benzyladenine (BA) and a- Naphthalene acetic acid (NAA). All microshoots formed roots and normal root morphology occurred on half strength MS salt supplied with 0.5 mg L-1 NAA or Indole-B-Butyric acid (IBA). Rooted microshoots (95 %) were successfully transferred to soil.

scholarly journals COMPARISON OF TWO METHODS FOR IN VITRO PROPAGATION OF RAUWOLFIA SERPENTINA FROM NODAL EXPLANTS

2007 ◽  
Vol 44 (07) ◽  
pp. 514-519 ◽  
Author(s):  
Ved Prakash Pandey ◽  
Jose Kudakasseril ◽  
Elizabeth Cherian ◽  
George Patani ◽  
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
In Vitro Multiplication ◽  
Rauwolfia Serpentina ◽  
In Vitro Micropropagation

Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. The plants produced were successfully hardened and established in soil. An easy, reliable and reproducible protocol was developed for in vitro micropropagation of Rauwolfia serpentina from nodal explants.

scholarly journals Challenges and Opportunity of in vitro Propagation of Paulownia tomentosa Steud for Commercial Production in Nepal

2016 ◽  
Vol 4 (2) ◽  
pp. 155-160
Author(s):  
Lila Bahadur Magar ◽  
Nisha Shrestha ◽  
Saraswoti Khadka ◽  
Jay Raj Joshi ◽  
Jibaraj Acharya ◽  
...  
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Royalty Rate ◽  
Paulownia Tomentosa ◽  
Shoot Bud ◽  
Plastic Bags

Paulownia tomentosa Steud is a fast growing multipurpose tree. In vitro propagation using nodal explants of Paulownia tomentosa was performed by manipulating amount of cytokinin and auxin in culture media. Shoot bud proliferation from explants were assessed in Murashige and Skoog (MS) medium supplemented with various amounts of hormones such as a) 0.5-2.5 mg/l benzylaminopurine (BAP) and 0.1 mg/l naphthalene acetic acid (NAA), b) 0.5-2.5 mg/l BAP and 0.5-2.5 mg/l kinetin (KN) and c) 0.5- 2.5 mg/l BAP and 0.1 mg/l indole-3-acetic acid (IAA). In the present study, we found that hormone combination of BAP and NAA gave optimum growth results. MS medium enriched with 2.0 mg/l BAP and 0.1 mg/l IAA resulted a similar outcome but took 3-4 weeks with respect to the same medium enriched with 1.0 mg/ml BAP and 0.1 mg/ml NAA, which showed response within 2-3 weeks. Shoot length of 2.5-3.5 cm with 3-4 nodes and 8-12 leaves were obtained on MS medium supplemented with 1.0 mg/l BAP and 0.1 mg/l NAA. The acclimatization of explants was done in a polyhouse at 20±5oC for 2-6 weeks. Rooting has been induced in nonsterile sand. Rooted plants were transferred to plastic bags containing mixture of soil, sand and compost in the ratio of 1:1:1.Besides aforementioned issues, there are several other challenges associated with in vitro propagation of P. tomentosa. The plants were established (90%) on MS medium enriched with BAP and NAA and adapted ex vitro with surviving up to 80%. People received an opportunity with this plant because it grows fast and can generate income in 10 years in comparison with others, but at the same time people also have been facing the challenges for plantation of P. tomentosa as government of Nepal does not formulate necessary national policies, legislations and regulatory frameworks in its favor. Thus, system should be developed to set royalty rate of P. tomentosa recognizing its lifetime value.Int J Appl Sci Biotechnol, Vol 4(2): 155-160

Rapid in vitro Propagation of Dodonaea viscosa (Sapindaceae) Through Axillary Bud Multiplication and Indirect Organogenesis

2015 ◽  
Vol 22 (1) ◽  
pp. 33-36
Author(s):  
A. Benniamin ◽  
G. Jothi ◽  
M. Sundari
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
High Rate ◽  
Axillary Bud ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Induction ◽  
Dodonaea Viscosa ◽  
Indirect Organogenesis

Protocols of axillary bud multiplication were established for Dodonaea viscosa (Sapindaceae). Murashig & Skoog’s medium with 0.2 mg/l BAP with 0.01 Naphthalene acetic acid induced high rate of shoot induction and an average of five shoots per node. Subsequent culture enhanced the number of shoots. Callus initiated from the basal cut end explants differentiated in to more than 10 shoots on MS Medium with 0.4mg/l Benzylaminopurine and 0.02mg/l Indole Butric Acid. Eighty per cent of the rooted shoots survived when transferred to greenhouse and subsequently to the field.

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