scholarly journals IN VITRO PROPAGATION OF ALGERIAN IVY (HEDERA CANARIENSIS L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1123g-1124
Author(s):  
Karim H. Al-Juboory ◽  
David J. Williams
Keyword(s):  
Root Length ◽  
In Vitro Propagation ◽  
Inverse Relationship ◽  
Basal Medium ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Tip Explants ◽  
Strength Medium

Shoot tip explants of Algerian Ivy Heder a canariensis were cultured on MS basal medium supplemented with a combination of salt strength and NAA and IBA. More roots per explant developed on full salt strength medium combined with NAA. The most roots per explant were obtained with a combination of IBA and 1/4 MS salt. There was an inverse relationship between an increase in IBA or NAA concentration and root length and number. Shoots proliferated better on full MS salt combined with NAA and IBA. The highest level of NAA (40 uM) and 0.1 uM TDZ produced the most shoots and roots, the longest roots, the highest rooting percentage, the largest plants with the most leaves and the best callus quality per explant. The leaves from in vitro were cultured on MS medium with varying levels of Thidiazuron (TDZ) and NAA in the presence of light produced the highest number of roots.

scholarly journals IN VITRO PROPAGATION OF ALGERIAN IVY (HEDERA CANARIENSIS L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1123G-1124 ◽  
Author(s):  
Karim H. Al-Juboory ◽  
David J. Williams
Keyword(s):  
Root Length ◽  
In Vitro Propagation ◽  
Inverse Relationship ◽  
Basal Medium ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Tip Explants ◽  
Strength Medium

Shoot tip explants of Algerian Ivy Heder a canariensis were cultured on MS basal medium supplemented with a combination of salt strength and NAA and IBA. More roots per explant developed on full salt strength medium combined with NAA. The most roots per explant were obtained with a combination of IBA and 1/4 MS salt. There was an inverse relationship between an increase in IBA or NAA concentration and root length and number. Shoots proliferated better on full MS salt combined with NAA and IBA. The highest level of NAA (40 uM) and 0.1 uM TDZ produced the most shoots and roots, the longest roots, the highest rooting percentage, the largest plants with the most leaves and the best callus quality per explant. The leaves from in vitro were cultured on MS medium with varying levels of Thidiazuron (TDZ) and NAA in the presence of light produced the highest number of roots.

scholarly journals In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aakriti Bhandari ◽  
Harminder Singh ◽  
Amber Srivastava ◽  
Puneet Kumar ◽  
G. S. Panwar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Average Length ◽  
Germplasm Conservation ◽  
Basic Chromosome Number ◽  
Shoot Tip ◽  
Ex Situ ◽  
Ms Medium ◽  
Basic Chromosome ◽  
Shoot Tip Explants

Abstract Background Sophora mollis Royle (family Fabaceae, subfamily-Papilionaceae) is a multipurpose legume distributed in plains and foothills of the North-West Himalaya to Nepal and is facing high risk of extinction due to habitat loss and exploitation by the local people for its fuel and fodder values. Therefore, the present study was conducted to standardize a micropropagation protocol for Sophora mollis by using shoot tip explants and to study the meiotic chromosome count in the species. Results Multiple shoots were induced in shoot tip explants of Sophora mollis in Murashige and Skoog medium supplemented with different concentrations of cytokinins alone (BAP, TDZ, and Kinetin) and in combination with varying concentrations of NAA. MS medium supplemented with BAP (8.9 μM) was observed to be the optimal medium for multiple shoot induction and maximum 25.32 shoots per explant was obtained with average length of 4.5 ± 0.8 cm. In vitro developed shoots were transferred onto rooting media supplemented with different concentrations of auxin (IAA, IBA, and NAA). Maximum 86% rooting was observed in half-strength MS medium supplemented with 21.20 μM NAA with an average of 21.26 roots per culture. In vitro raised plantlets were adapted to greenhouse for better acclimatization and 60% plants were successfully transferred to the open environment. Based on the chromosome counts available from the literature and the current study, the species tend to show a basic chromosome number of x = 9. Conclusion The micropropagation protocol standardized can be helpful for the ex situ mass multiplication and germplasm conservation of the endangered species. Moreover, the ex situ conservation approach will be helpful in actively bridging the gap between ex situ and in situ approaches through the reintroduction of species in the wild. The cytological studies revealed the basic chromosome number x = 9 of the species.

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals In Vitro Propagation of Eriostemon myoporoides and Eriostemon `Stardust'

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 686-688 ◽  
Author(s):  
James R. Ault
Keyword(s):  
Root Length ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Length ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
Axillary Shoot Proliferation

Optimal axillary shoot proliferation was obtained from stem explants of a clone of Eriostemon myoporoides DC. on Murashige and Skoog (MS) basal medium containing 0.1 mg BA/liter, and of Eriostemon `Stardust' on MS medium containing 0.5 mg BA/liter. Overall average number of shoots and shoot lengths for all treatments was greater for E. `Stardust' (22.4 shoots and 12.1-mm shoot length) than for E. myoporoides (4.5 shoots and 8.3-mm shoot length). Maximum percent rooting of E. myoporoides (42%) and E. `Stardust' (95%) was obtained on MS medium supplemented with 1.0 mg K-IBA/liter for E. myoporoides and 0.1 mg NAA/liter for E. `Stardust'. Overall average percent rooting and root lengths were greater for E. `Stardust' (42% rooting and 11.0-mm root length) than for E. myoporoides (27% rooting and 2.3-mm root length). For E. `Stardust', reducing sucrose in the rooting medium from 50 to 25 g·liter-1 significantly decreased overall average percent rooting to 1670 and root length to 6.8 mm. Plantlets of both clones were acclimatized in the greenhouse and transferred successfully to soil, although survival was <7070. Chemical names used: N -(phenylmethyl) -l H -purine-6-amine (BA); potassium-l H -indole-3-butyric acid (K-IBA); l-naphthaleneacetic acid (NAA).

scholarly journals In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants

2009 ◽  
Vol 18 (1) ◽  
pp. 1-6 ◽  
Author(s):  
H. David Raja ◽  
D.I. Arockiasamy
Keyword(s):  
High Frequency ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Root Induction ◽  
Root Proliferation ◽  
Key Words Micropropagation ◽  
Shoot Tip Explants ◽  
Important Medicinal Plant

A simple micropropagation method from nodal and shoot tip explants is reported here for Mentha viridis, an economically important medicinal plant. High frequency of microshoots was obtained from these two explants on MS supplemented with various concentrations of BAP (1.0 - 4.0 mg/l) and Kn (1.0 -4.0 mg/l). Maximum number of shoots was obtained from nodal explants in the medium containing 3.0 mg/l BAP. The root induction was carried out by using IBA and IAA (0.5 - 2.0 mg/l). Among these, high frequency of root proliferation was achieved in the medium containing 1.5 mg/l of IBA. The rooted plantlets were hardened and transferred to the field. The survival rate was 90 - 95% after 25 days. Key words: Micropropagation, Mentha viridis, Node, Shoot tip, Multiple shoots D.O.I. 10.3329/ptcb.v18i1.3243 Plant Tissue Cult. & Biotech. 18(1): 1-6, 2008 (June)

scholarly journals Plant Regeneration from Leaf Explants of the Medicinal Herb Wedelia chinensis

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 407
Author(s):  
Yung-Ting Tsai ◽  
Kin-Ying To
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Liver Toxicity ◽  
Leaf Explants ◽  
Basal Medium ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Average Percentage

Wedelia chinensis, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of W. chinensis by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA3), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of W. chinensis in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant W. chinensis.

scholarly journals Factors Influencing in vitro Germination and Seed Storage Behavior of Couroupita guianensis Aubl. – A Useful Tropical Tree Species

2018 ◽  
Vol 15 (4) ◽  
pp. 957-968
Author(s):  
P. Shiny Arokiamary ◽  
A. Vinoth Alphonse ◽  
R. Ravindhran
Keyword(s):  
Root Length ◽  
Basal Medium ◽  
Seed Storage ◽  
Ms Medium ◽  
Embryo Germination ◽  
Natural Habitats ◽  
Couroupita Guianensis ◽  
Basal Salts ◽  
Mature Seeds

Couroupita guianensis Aubl. popularly known as cannonball tree is widely distributed in the tropical regions. The tree parts are commonly used to treat wounds and tumors. Leaves, flowers, and fruits contain active phytochemicals with significant biological activity. In the recent years, destruction of natural habitats by mankind has reduced its distribution. Natural propagation of C. guianensis by seeds is greatly hindered by poor seed germination and viability. Therefore, the present study was undertaken to optimize the conditions for in vitro embryo germination and to investigate the seed storage behaviour. Mature seeds inoculated on MS basal medium germinated within 10 d with a frequency of 61.6%. Supplementation of plant growth regulators (PGRs) to MS medium improved the embryo germination frequency (100%). Seedlings with highest shoot length (8.10±0.11 cm) and root length (6.27±0.14 cm) were produced in MS medium supplemented with 1.0 mg/l kinetin and 0.1 mg/l indole-3-butyric acid. Among different strength liquid MS basal salts, quarter-strength produced a greater number of secondary roots (8.00±0.28) with average root length of 17.83±0.58 cm. Seed storage behaviour studies clearly proved the recalcitrant nature as only freshly harvested mature seeds retained the germination potential upon storage at 15 ºC for up to 45 d. Desiccation of seeds on exposure to air-dry storage resulted in rapid deterioration of germination. Pre-conditioning of germinated seedlings in liquid MS basal salts was required for their survival under field conditions. Plantlets with well-developed roots were successfully acclimatized to the field with 100% survivability. This protocol facilitates conservation, sustainable utilization and re-introduction of C. guianensis into its natural habitats.

scholarly journals Thidiazuron and Benzylamino Purine Stimulation of Apple Shoot Proliferation in Vitro

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 629e-629
Author(s):  
Karim H. Al-Juboory ◽  
Jabar H. Al-Niami
Keyword(s):  
Malus Domestica ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Ms Medium ◽  
Proliferation In Vitro ◽  
Shoot Tip Explants ◽  
Apple Shoot ◽  
Stimulation Of

Thidiazuron (TDZ) and benzylamino purine stimulated shoot proliferation on shoot tip explants of wild apple (Malus domestica Borkh) when incorporated in Murashige and Skoog (MS) medium at concentrations of 1.0–10 μm. Shoot numbers obtained with TDZ were greater than the number produced when using BA in the medium but the shoots were shorter than with BA. Increasing TDZ levels increased shoot proliferation with 10 μm. Apple shoots were successfully rooted on MS medium with 2.0 mg·L–1 NAA and then transferred to a mixture of 1 peat: 1 perlite: 1 soil and acclimatized for potting.

scholarly journals In Vitro Propagation of Apios americana

HortScience ◽  
1990 ◽  
Vol 25 (11) ◽  
pp. 1439-1440 ◽  
Author(s):  
E.R.M. Wickremesinhe ◽  
W.J. Blackmon ◽  
B.D. Reynolds
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Axillary Buds ◽  
Ms Medium ◽  
Butanoic Acid ◽  
Apios Americana

Shoot proliferation from axillary buds of Apios americana Medikus (apios, groundnut) was obtained on a modified Murashige and Skoog (MS) medium supplemented with 2.22 μm BAP, 0.5 μm IBA, and 3.0 μm GA3. Existed shoots rooted on MS basal medium. About 60% of the rooted plants were successfully established in soil. Chemical names used: 1 H-indole-3-butanoic acid (IBA). gibberellic acid (GA3), N6-benzylaminopurine (BAP).

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