Modified natural cycle frozen-thawed embryo transfer in patients with repeated implantation failure: An observational study

Abstract Background Displacement of the window of implantation (WOI) has been proposed as an important factor contributing to repeated implantation failure (RIF). However, the use of histologic endometrial dating as a diagnostic tool of endometrial receptivity has been questioned. Methods This study is a prospective intervention trial that enrolled 205 infertile patients from July 2017 to December 2017. Endometrial biopsies from 50 patients with good prognoses were conducted on day 3 (n = 6), 5 (n = 6), 7 (n = 26), 9 (n = 6), or 11 (n = 6) post-ovulation (PO + 3/5/7/9/11) of the previous natural cycle before their conventional frozen-thawed embryo transfer (FET) cycle. We conducted endometrial biopsies for 155 RIF patients on day PO + 7. Results The verification of the Noyes criteria for endometrial dating was conducted at different times (PO + 3/+ 5/+ 7/+ 9/+ 11) on 41 patients with good prognoses who achieved an ongoing pregnancy in their first conventional FET cycle after endometrial biopsy. The agreement between two pathologists determining endometrial biopsy dating results in infertile patients was determined to be acceptable (weighted kappa = 0.672, P < 0.001). The rate of out-of-phase dating on day PO + 7 was significantly higher in RIF patients than in good- prognosis patients (31.6% vs. 3.8%, P = 0.003). pFET was performed in 47 RIF patients diagnosed to be out of phase, and the cumulative live-birth rate was 61.7%. Conclusions Histologic endometrial dating of RIF patients in natural cycles may be a biomarker for a receptive endometrium in diagnosing WOI displacement. Trial registration NCT03312309 Registered 17 October 2017. NCT03222830 Registered 19 July 2017,

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Analysis of the guidance value of 3D ultrasound in evaluating endometrial receptivity for frozen-thawed embryo transfer in patients with repeated implantation failure

Annals of Translational Medicine ◽

10.21037/atm-20-5463 ◽

2020 ◽

Vol 8 (15) ◽

pp. 944-944 ◽

Cited By ~ 1

Author(s):

Ruiqing Tong ◽

Ying Zhou ◽

Qi He ◽

Yanyan Zhuang ◽

Weiqin Zhou ◽

...

Keyword(s):

Embryo Transfer ◽

3D Ultrasound ◽

Endometrial Receptivity ◽

Implantation Failure ◽

Repeated Implantation Failure ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

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Injury to the endometrium prior to the frozen-thawed embryo transfer cycle improves pregnancy rates in patients with repeated implantation failure

Journal of Obstetrics and Gynaecology Research ◽

10.1111/jog.13182 ◽

2016 ◽

Vol 43 (1) ◽

pp. 128-134 ◽

Cited By ~ 8

Author(s):

Emiko Kanazawa ◽

Akira Nakashima ◽

Koji Yonemoto ◽

Misako Otsuka ◽

Naomi Yoshioka ◽

...

Keyword(s):

Embryo Transfer ◽

Pregnancy Rates ◽

Implantation Failure ◽

Repeated Implantation Failure ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

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THE EFFECT OF ASSISTED HATCHING IN FROZEN-THAWED EMBRYO TRANSFER CYCLES

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2016.3.12 ◽

2016 ◽

pp. 80-84

Author(s):

Thi Tam An Nguyen ◽

Minh Tam Le ◽

Ngoc Thanh Cao

Keyword(s):

Embryo Transfer ◽

Zona Pellucida ◽

Assisted Hatching ◽

Implantation Failure ◽

Multiple Pregnancies ◽

Recurrent Implantation Failure ◽

Key Words Laser ◽

Implantation And Pregnancy Rates ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

Background: Laser assisted hatching technique based on the hypothesis to make an artificial hole on zona pellucida (ZP) that can help embryo hatching out of ZP easily. This technique has been shown to increase implantation and pregnancy rates in women of advanced age, in women with recurrent implantation failure and following the transfer of frozen–thawed embryos. This study described the outcome of frozen–thawed embryo transfers with laser assisted hatching (LAH), which is one of the safest method in nowadays. Purpose: To assess the effect of assisted hatching technique on the clinical outcomes in vitrified-warmed transfer cycles. Method: A total of 65 thawed-transfer cycles with 153 thawed-embryos undertaken within a 12-month period were analysed, Assisted hatching with laser zona thinning was performed with one-quarter of the zona pellucida circumference. The overall thawed-embryos (day 3) were kept in culture overnight. Patient were prepared the suitable endometrium and transferred embryos advantageously. Results: In which, having the rate of survival embryos were 143 occupying 94.3%, the percentage of grade 1 and 2 embryos occupied 55.9% and 29,3% respectively, and that were enrolled LAH before transfering of frozen–thawed embryos. The average transferred embryos were 2.4±0.8, The rate of implantation per transferred embryos and per transferred embryos cycles was 19.5% and 43.1% respectively. The rate of clinical pregnancies per embryo transfer cycles occupied 33.8% with percentage of early miscarriages (biochemical pregnancies and early clinical miscarriages) was 12.3%. The rate of ongoing pregnancies was 30.8% and multiple pregnancies was low just 12.3%. This result was equal or higher than other researchs in embryos transfer had or no LAH. Conclusion: LAH contributed to stable frozen–thawed embryos transfer effectiveness. Key words: Laser assisted hatching, frozen–thawed embryos transfer, zona pellucida (ZP)

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