scholarly journals Role of bovine respiratory syncytial virus in etiology of respiratory diseases on milk farms

2021 ◽  
Vol 1 (1) ◽  
pp. 38-43
Author(s):  
S. V. Koteneva ◽  
A. V. Nefedchenko ◽  
T. I. Glotova ◽  
A. G. Glotov

Bovine respiratory syncytial virus (BRSV) is one of the etiological agents of respiratory diseases. The agent spreads widely in all the countries with intensive livestock farming and can cause pathologic changes in respiratory system either alone or in combination with other viruses and bacteria. It is a matter of crucial importance to study spread of the agent on large milk farms, to detect it in the internal organs of infected animals, and to quantify virus accumulation in them. The purpose of the research was to study peculiarities of RS infection spread, frequency of the virus detection in biomaterial samples (both alone and in associations with infectious bovine rhinotracheitis (IBR) and bovine viral diarrhea/mucosal disease viruses (BVDV) and with Pasteurellaceae bacteria) on large milk farms affected by respiratory animal diseases; and to determine virus concentration in the respiratory organs. BRSV alone was reported in 9.2% of the tested biomaterial samples, as associated with IBR and BVDV it was reported in 1.4% and 5.2% of samples, correspondingly. The number of samples containing simultaneously BRSV and Pasteurellaceae bacteria was 10.8%. The virus was reported in a maximum of 26.6% of the tested samples. With the help of real-time PCR the virus genome was detected in lungs (13.1%), in exudate from trachea, bronchi and nasal sinuses (6.0%), in nasal discharge (4.0%) and in bronchi (1.7%). The virus was seldom detected in trachea and bronchial mucosa (1.1%) and in pulmonary lymph nodes (0.8%). Quantification of BRSV RNA demonstrated that maximum virus accumulation was observed in lungs and nasal charges and it confirms data on its tropism to pulmonary interstitium.

2007 ◽  
Vol 88 (4) ◽  
pp. 1260-1265 ◽  
Author(s):  
Martine Deplanche ◽  
Mylène Lemaire ◽  
Carole Mirandette ◽  
Marion Bonnet ◽  
François Schelcher ◽  
...  

We analysed the genetic evolution of bovine respiratory syncytial virus (BRSV) isolate W2-00131, from its isolation in bovine turbinate (BT) cells to its inoculation in calves. Results showed that the BRSV genomic region encoding the highly variable glycoprotein G remained genetically stable after virus isolation and over 10 serial infections in BT cells, as well as following experimental inoculation in calves. This remarkable genetic stability led us to examine the mutant spectrum of several populations derived from this field isolate. Sequence analysis of molecular clones revealed an important genetic heterogeneity in the G-coding region of each population, with mutation frequencies ranging from 6.8 to 10.1×10−4 substitutions per nucleotide. The non-synonymous mutations of the mutant spectrum mapped preferentially within the two variable antigenic regions of the ectodomain or close to the highly conserved domain. These results suggest that BRSV populations may evolve as complex and dynamic mutant swarms, despite apparent genetic stability.


2006 ◽  
Vol 116 (1-2) ◽  
pp. 30-37 ◽  
Author(s):  
Fernando Rosado Spilki ◽  
Renata Servan Almeida ◽  
Helena Gallichio Domingues ◽  
Regina Celia Freitas D’Arce ◽  
Helena Lage Ferreira ◽  
...  

1996 ◽  
Vol 53 (3-4) ◽  
pp. 253-260 ◽  
Author(s):  
R.S. Schrijver ◽  
F. Daus ◽  
J.A. Kramps ◽  
J.P.M. Langedijk ◽  
R. Buijs ◽  
...  

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