Identification Antigenic Protein of Larva Rhipicephalus Sanguineus By Western Blot Technique and Humoral Immune Response In Rabbits Immunized Protein Larvae Of Rhipicephalus Sanguineus

Se comparó la respuesta inmune humoral inducida por las proteínas estructurales del virus de la estomatitis vesicular (serotipos Indiana y New Jersey), utilizando sueros de bovinos vacunados e infectados natural y experimentalmente. Para ello, se usó la técnica de inmunotransferencia (Western blot) a través de la cual se observó el reconocimiento exclusivo de la proteína G en sueros de animales vacunados, así como de las proteínas G y N en sueros de animales infectados. Los sueros de los animales infectados experimentalmente presentaron reacción cruzada en el caso de la proteína N. Se realizaron corridos electroforéticos y densitométricos de 13 subclones del virus de la estomatitis vesicular aislados en distintas áreas endémicas del país. Los resultados mostraron una homología completa e dependiente de su lugar de origen, lo cual es coherente con la literatura reportada. Abstract Comparison between the immune response to vesicular stomatitis proteins virus in vaccinated bovine and naturally or induced infected onesThe induced humoral immune response against structural proteins of Indiana and New Jersey serotypes of the vesicular stomatitis virus was compared using serum obtained from vaccinated bovine, natural and experimentally infected ones. It was used Western blot technique to observe the recognition of protein G by serum of vaccinated animals, and the response to proteins G and N by serum of infected animals. Serum of experimentally infected animals showed crossed reaction for protein N.Were carried out electrophoretic and densitometric analysis on 13 vesicular stomatitis virus subclons isolated from various endemic areas of the country.The results showed complete homology, independent from their origin ccording to the reported literature.

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The Evaluation of Humoral Immune Response to Contagious Bovine Pleuropneumonia in Cattle with Respiratory Disorders by Western Blot Technique

Dicle Üniversitesi Veteriner Fakültesi Dergisi ◽

10.47027/duvetfd.995044 ◽

2021 ◽

Author(s):

Sevil ERDENLİĞ GÜRBİLEK ◽

Ümit ÖZDEMİR ◽

Osman Yaşar TEL

Keyword(s):

Immune Response ◽

Western Blot ◽

Humoral Immune Response ◽

Contagious Bovine Pleuropneumonia ◽

Respiratory Disorders ◽

Humoral Immune ◽

Blot Technique

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Humoral Immune Response of Dogs Immunized with Salivary Gland, Midgut, or Repeated Infestations with Rhipicephalus sanguineus

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.2000.tb05302.x ◽

2006 ◽

Vol 916 (1) ◽

pp. 283-288 ◽

Cited By ~ 6

Author(s):

SATHAPORN JITTAPALAPONG ◽

ROGER W. STICH ◽

JOHN C. GORDON ◽

CATHERINE A. BREMER ◽

OMAR O. BARRIGA

Keyword(s):

Immune Response ◽

Salivary Gland ◽

Humoral Immune Response ◽

Rhipicephalus Sanguineus ◽

Humoral Immune

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Western blot analysis of humoral immune response to Leishmania donovani antigens in patients with post-kala-azar dermal leishmaniasis

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1016/s0035-9203(99)90197-9 ◽

1999 ◽

Vol 93 (1) ◽

pp. 98-101 ◽

Cited By ~ 17

Author(s):

Poonam Salotra ◽

Anita Raina ◽

V. Ramesh

Keyword(s):

Immune Response ◽

Western Blot ◽

Humoral Immune Response ◽

Blot Analysis ◽

Western Blot Analysis ◽

Leishmania Donovani ◽

Humoral Immune ◽

Kala Azar

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Trypanosoma cruzi in marsupial didelphids (Philander frenata and Didelhis marsupialis): differences in the humoral immune response in natural and experimental infections

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822003000200008 ◽

2003 ◽

Vol 36 (2) ◽

pp. 241-248 ◽

Cited By ~ 9

Author(s):

Ana Paula Legey ◽

Ana Paula Pinho ◽

Samanta C.C. Xavier ◽

Renato Marchevsky ◽

João Carlos Carreira ◽

...

Keyword(s):

Immune Response ◽

Trypanosoma Cruzi ◽

Western Blot ◽

Humoral Immune Response ◽

Immunofluorescence Assay ◽

Evolutionary Divergence ◽

Humoral Immune ◽

Recognition Pattern ◽

Sds Page

Philander frenata and Didelphis marsupialis harbor parasitism by Trypanosoma cruzi without developing any apparent disease and on the contrary to D. marsupialis, P. frenata maintains parasitism by T. cruzi II subpopulations. Here we compared the humoral immune response of the two didelphids naturally and experimentally infected with T. cruzi II group, employing SDS-PAGE/Western blot techniques and by an Indirect immunofluorescence assay. We also studied the histopathological pattern of naturally and experimentally infected P. frenata with T. cruzi. P. frenata sera recognized more antigens than D. marsupialis, and the recognition pattern did not show any change over the course of the follow up of both didelphid species. Polypeptides of 66 and 90kDa were the most prominent antigens recognized by both species in the soluble and enriched membrane fractions. P. frenata recognized intensely also a 45kDa antigen. Our findings indicate that: 1) there were no quantitative or qualitative differences in the patent or subpatent phases in the recognition pattern of P. frenata; 2) the significant differences in the recognition pattern of parasitic antigens by P. frenata and D. marsupialis sera suggest that they probably "learned" to live in harmony with T. cruzi by different strategies; 3) although P. frenata do not display apparent disease, tissular lesions tended to be more severe than has been described in D. marsupialis; and 4) Both didelphids probably acquired infection by T. cruzi after their evolutionary divergence.

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Quantitative and qualitative analysis of sea bream,Sparus aurata(L.), humoral immune response, vaccinated with commercial and experimental vaccines against vibriosis and photobacteriosis

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315417001631 ◽

2017 ◽

Vol 98 (1) ◽

pp. 105-115 ◽

Cited By ~ 2

Author(s):

Vasileios Bakopoulos ◽

Ioanna Kosma ◽

Evita Laspa

Keyword(s):

Immune Response ◽

Western Blot ◽

Humoral Immune Response ◽

Blot Analysis ◽

Western Blot Analysis ◽

Sparus Aurata ◽

Sea Bream ◽

Whole Cell ◽

Humoral Immune ◽

Commercial Vaccine

The specific humoral immune response of sea bream,Sparus aurata(L.), againstVibrio anguillarumO1 andPhotobacterium damselaesubsp.piscicida(Phdp) after immunization with commercial and experimental bacterins was analysed quantitatively and qualitatively. Specific anti-V. anguillarumO1 and anti-Phdp levels provoked by the adjuvanted commercial vaccine reached higher levels in comparison to the aqueous commercial and experimental bacterins. Infection of vaccinated fish withV. anguillarumO1 bacterial cells acted as a boost of the humoral immune response, except for the sera of the group vaccinated with the adjuvanted vaccine. Infection with Phdp acted as a boost of the humoral immune response mainly for the group vaccinated with a monovalent Phdp bacterin and to a lesser degree for the group vaccinated with the aqueous commercial vaccine. Western blot analysis of the sera againstV. anguillarumO1 whole cell antigens revealed strong reactions to only a few antigens below 54 kD and above 15 kD and weak reactions to other antigens. Similar reactions were observed from the sera isolated from the controls. Western blot analysis of the sera against Phdp whole cell antigens revealed strong reactions to only a handful of antigens below 20.7 and below 6.4 kD. Sera from the control group, as in the case ofV. anguillarumO1, reacted with Phdp whole cell antigens. No differences regarding antigen reactions between monovalent and bivalent formulations were noted, in contrast to the adjuvanted and aqueous bacterins.

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