scholarly journals Isolation and detection of rotavirus by Enzyme linked Immune assay in fecal specimens of buffalo calves

2019 ◽  
Vol 42 (2) ◽  
pp. 1-6
Author(s):  
Ahmed Saadi Khalaf
Keyword(s):  
Cell Culture ◽  
Immunosorbent Assay ◽  
Kidney Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Buffalo Calves ◽  
Bovine Kidney ◽  
Kidney Cell Culture ◽  
Infected Cells ◽  
Fetal Bovine ◽  
Cytopathogenic Effect

A total of 50 fecal samples were collected from buffalo calves ages between 3 days to 4 months, in middle area of Iraq (Baghdad, Salahaldden, Babylon, Diyala, and Wasit) between July 2016 to June 2017. All samples were examined by Immuno-chromatographic rapid tests for detection of buffalo rotavirus, twenty samples were positive. Enzyme-Linked Immunosorbent Assay. test was also used and  revealed only 8  positive samples, the later samples   were used for viral isolation on  fetal bovine kidney cell culture for detection the cytopathogenic  effects of the virus. The cytopathogenic effects of virus were induced rounded cells and gaps; as a result of rupture of these infected cells by rotavirus. Moreover Enzyme-Linked Immunosorbent Assay  test used again for detection of rotavirus cytopathogenic effect in fetal bovine kidney cell culture for more confirmation. The TCID 50 was estimated for rotavirus on fetal bovine kidney cells 104.5 virus/0.05 ml

Internalisation of calcitonin receptor complexes in primary rat kidney cell culture

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S1
Author(s):  
H.-G. SCHNEIDER ◽  
F. RAUE ◽  
J. SCHROTH ◽  
H. SCHERÜBL ◽  
R. ZIEGLER
Keyword(s):  
Cell Culture ◽  
Kidney Cell ◽  
Rat Kidney ◽  
Calcitonin Receptor ◽  
Receptor Complexes ◽  
Kidney Cell Culture

scholarly journals Isolation of peste des petits ruminants virus using primary goat kidney cell culture from kidneys obtained at slaughter

2020 ◽  
Author(s):  
Shahana Begum ◽  
Mohammed Nooruzzaman ◽  
Azmary Hasnat ◽  
Mst. Murshida Parvin ◽  
Rokshana Parvin ◽  
...  
Keyword(s):  
Cell Culture ◽  
Kidney Cell ◽  
Peste Des Petits Ruminants ◽  
Kidney Cell Culture

scholarly journals Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Antibody to Epizootic Hemorrhagic Disease of Deer Virus

2000 ◽  
Vol 12 (2) ◽  
pp. 142-145 ◽  
Author(s):  
James O. Mecham ◽  
Michael M. Jochim
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hemorrhagic Disease ◽  
Structural Protein ◽  
Serum Samples ◽  
Epizootic Hemorrhagic Disease ◽  
Diagnostic Potential ◽  
The Us ◽  
Serotype 1 ◽  
Infected Cells

An enzyme-linked immunosorbent assay has been developed to detect antibodies to epizootic hemorrhagic disease of deer virus (EHDV). The assay incorporates a monoclonal antibody to EHDV serotype 2 (EHDV-2) that demonstrates specificity for the viral structural protein, VP7. The assay was evaluated with sequential sera collected from cattle experimentally infected with EHDV serotype 1 (EHDV-1) and EHDV-2, as well as the four serotypes of bluetongue virus (BTV), BTV-10, BTV-11, BTV-13, and BTV-17, that currently circulate in the US. A competitive and a blocking format as well as the use of antigen produced from both EHDV-1-and EHDV-2-infected cells were evaluated. The assay was able to detect specific antibody as early as 7 days after infection and could differentiate animals experimentally infected with EHDV from those experimentally infected with BTV. The diagnostic potential of this assay was demonstrated with field-collected serum samples from cattle, deer, and buffalo.

A Herpesvirus Isolated from Kidney Cell Culture of Normal Turkeys

1969 ◽  
Vol 13 (4) ◽  
pp. 853 ◽  
Author(s):  
H. Kawamura ◽  
D. J. King ◽  
D. P. Anderson
Keyword(s):  
Cell Culture ◽  
Kidney Cell ◽  
Kidney Cell Culture
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