Identification of an exogenous retrovirus (foamy virus type 1) in Rhesus monkey kidney cell culture: significance to viral diagnostics

1998 ◽  
Vol 11 (2) ◽  
pp. 149-153 ◽  
Author(s):  
S.M. Lipson ◽  
M.E. Match ◽  
D.H. Shepp ◽  
M.S. Lotlikar ◽  
S. Teichberg ◽  
...  
1986 ◽  
Vol 42 (6) ◽  
pp. 749-760 ◽  
Author(s):  
G. Wijngaards ◽  
D.C. Rijken ◽  
A.L. van Wezel ◽  
E. Groeneveld ◽  
C.A.M. Van der Velden

1969 ◽  
Vol 15 (5) ◽  
pp. 451-454 ◽  
Author(s):  
J. Joncas ◽  
A. Chagnon ◽  
G. Lussier ◽  
V. Pavilanis

A human fetal lung cell culture contaminant possessing most of the characteristics of that described by Girardi et al. (5) was shown to belong to the M. hyorhinis-GDL group of cytopathic mycoplasma by accessory factor neutralization test. The homotypic antiserum in this test neutralized the contaminant as well as three closely related strains while it could inhibit the growth of the contaminant only on agar. The mycoplasma could be detected in tissue cultures by the acridine orange staining technique and by direct immunofluorescence. These appeared as minute coccobacillary bodies either isolated or in clumps, predominantly extracellular in the original tissue culture but apparently intracellular in monkey kidney cell culture. A titer of 106 TCID50 per 0.1 ml was obtained in monkey kidney cell culture and the cytopathic effect induced in this culture was inhibited by pretreatment of the agent with 20% ether for 18 hours at 4 C, with tris buffer at pH 3 for 3 hours, or by heating at 56 C for 3 minutes. The cytopathic effect was suppressed by chlortetracycline (50 μg/ml) and neutralized with accessory factor by specific antiserum produced in rabbit up to a titer of 8192, whereas a variety of normal human and animal sera failed to do so. The source of the contaminant could not be determined.


1980 ◽  
Vol 19 (4-5) ◽  
pp. 525-534 ◽  
Author(s):  
D.M. Allison ◽  
J.W. Dirksen ◽  
R.F. Schmelter ◽  
M.C. Makoid ◽  
W.J. Murray

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