Optimization of Agrobacterium-Mediated Transient Expression of Immunocytokine Based on Human Interferon-α-2b and Anti-HER2 Antibody in Nicotiana Benthamiana

2020 ◽  
Vol 75 (5) ◽  
pp. 280-285
Author(s):  
K. M. Konoplina ◽  
E. N. Kosobokova ◽  
E. V. Sheshukova ◽  
M. V. Pinyugina ◽  
A. A. Malchenkova ◽  
...  
Keyword(s):  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Development of Fusion Protein Produce Technology Based on a Humanized Monoclonal Antibody Specific to Tumor Antigen HER2 and a Recombinant Human Interferon-α-2b in Nicotiana benthamiana

2019 ◽  
pp. 49-57 ◽  
Author(s):  
E.V. Sheshukova ◽  
M.V. Pinyugina ◽  
K.M. Konoplina ◽  
V.S. Kosorukov
Keyword(s):  
Monoclonal Antibody ◽  
Fusion Protein ◽  
Transient Expression ◽  
Tumor Antigen ◽  
Nicotiana Benthamiana ◽  
Fusion Proteins ◽  
Human Interferon ◽  
Interferon Α ◽  
Humanized Monoclonal Antibody ◽  
Human Interferon Α

Fusion proteins based on a recombinant human interferon-α-2b and a humanized monoclonal antibody specific to tumor antigen HER2 have been obtained by transient expression in Nicotiana benthamiana. To provide an effective expression and a proper immunocytokine molecule assembly the genetic constructs optimization was performed. The expression level was shown to depend on the linker length between the cytokine and the antibody moieties in the hybrid molecule. The modification of the interferon domain sequence by replacing cysteine residues with serine showed a slight increase in the production level of the target protein. The assembly of full-size fusion protein molecules was confirmed by the qualitative method of «combined ELISA» developed by the authors. The preservation of the affine domain function in the fusion proteins was proved by the ELISA method in interaction with the antigen. interferon- α-2b, antigen HER2, plant transient expression, recombinant fusion proteins.

scholarly journals Generation of transgenic fibroblasts producing doxycycline-inducible human interferon-α or erythropoietin for a bovine mammary bioreactor

2012 ◽  
Vol 12 (1) ◽  
pp. 1137-1144 ◽  
Author(s):  
HEE YOUNG KANG ◽  
EUI-JU HONG ◽  
KYU-CHAN HWANG ◽  
NAM-HYUNG KIM ◽  
WOO-SUK HWANG ◽  
...  
Keyword(s):  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Long-term stabilization of recombinant human interferon α 2b in aqueous solution without serum albumin

2003 ◽  
Vol 264 (1-2) ◽  
pp. 57-72 ◽  
Author(s):  
Llamil Ruiz ◽  
Nuria Reyes ◽  
Ladys Duany ◽  
Abrisleyda Franco ◽  
Kethia Aroche ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Serum Albumin ◽  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Human interferon-α induces immobility in the mouse forced swimming test: involvement of the opioid system

2000 ◽  
Vol 852 (2) ◽  
pp. 482-484 ◽  
Author(s):  
Mitsuhiro Makino ◽  
Yutaka Kitano ◽  
Chika Komiyama ◽  
Masaaki Hirohashi ◽  
Morihiro Kohno ◽  
...  
Keyword(s):  
Forced Swimming Test ◽  
Forced Swimming ◽  
Opioid System ◽  
Human Interferon ◽  
Interferon Α ◽  
Swimming Test ◽  
Human Interferon Α

A genetic study of human interferon-α-induced repair of DNA damage in hepatitis B patients

1991 ◽  
Vol 262 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Peilin Wang ◽  
Shuying Cong ◽  
Xiaoyong Dong ◽  
Zaichun Chen ◽  
Chuiping Ma
Keyword(s):  
Dna Damage ◽  
Hepatitis B ◽  
Genetic Study ◽  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Monoclonal Antibodies for Purification and RIA of Natural and Recombinant Human Interferon-α, -β And-γ

1985 ◽  
pp. 945-948
Author(s):  
D. NOVICK ◽  
D.G. FISCHER ◽  
Y. CHERNAJOVSKY ◽  
M. REVEL ◽  
M. RUBINSTEIN ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Human Interferon-α Receptor: Identification of the Region Involved in Binding to Interferon-αB

1996 ◽  
Vol 16 (10) ◽  
pp. 845-852 ◽  
Author(s):  
MARIO H. HIRATA ◽  
DAN SACKETT ◽  
ROSARIO D.C. HIRATA ◽  
NGA Y. NGUYEN
Keyword(s):  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α
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