Damage to Reissner's Membrane in the Guinea-Pig Cochlea Following Acute Atoxyl Intoxication

1976 ◽  
Vol 81 (3-6) ◽  
pp. 415-423 ◽  
Author(s):  
M. Anniko
Keyword(s):  
Guinea Pig ◽  
Guinea Pig Cochlea ◽  
Reissner's Membrane ◽  
Reissner’S Membrane

A cationic nonselective stretch-activated channel in the Reissner’s membrane of the guinea pig cochlea

1998 ◽  
Vol 274 (3) ◽  
pp. C566-C576 ◽  
Author(s):  
Te-Huei Yeh ◽  
Philippe Herman ◽  
Ming-Cheng Tsai ◽  
Patrice Tran Ba Huy ◽  
Thierry Van Den Abbeele
Keyword(s):  
Guinea Pig ◽  
Kinetic Studies ◽  
Boltzmann Distribution ◽  
Patch Clamp Technique ◽  
Open Probability ◽  
Guinea Pig Cochlea ◽  
Open Time ◽  
Current Voltage ◽  
Reissner's Membrane ◽  
Reissner’S Membrane

The Reissner’s membrane (RM) separates in the mammalian cochlea the K+-rich endolymph from the Na+-rich perilymph. The patch-clamp technique was used to investigate the transport mechanisms in epithelial cells of RM freshly dissected from the guinea pig cochlea. This study shows a stretch-activated nonselective cationic channel (SA channel) with a linear current-voltage relationship (23 pS) highly selective for cations over anions [K+ ≈ Na+ (1) > Ba2+ (0.65) > Ca2+ (0.32) ≫ Cl− (0.14)] and activated by the intrapipette gradient pressure. The open probability-pressure relationship is best fitted by a Boltzmann distribution (half-maximal pressure = 37.8 mmHg, slope constant = 8.2 mmHg). SA channels exhibit a strong voltage dependency and are insensitive to internal Ca2+, ATP, and fenamates but are blocked by 1 μM GdCl3 in the pipette. They are reversibly activated by in situ superfusion of the cell with hyposmotic solutions. Kinetic studies show that depolarization and mechanical or osmotic stretch modify the closed and open time constants probably by a different mechanism. These channels could participate in pressure-induced modifications of ionic permeability of the RM.

Effects of Combined Rupture of Reissner's Membrane and the Round Window on Hearing in the Guinea Pig

1994 ◽  
Vol 114 (sup514) ◽  
pp. 55-58 ◽  
Author(s):  
Kiyofumt Gyo ◽  
Taisuke Kobayashi ◽  
Naoaki Yanagihara
Keyword(s):  
Guinea Pig ◽  
Round Window ◽  
Reissner's Membrane ◽  
Reissner’S Membrane
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