Overexpression of CpWRKY75 from Chimonanthus praecox Promotes Flowering Time in Transgenic Arabidopsis

WRKY transcription factors play critical roles in the physiological processes of plants. Although the roles of WRKYs have been characterized in some model plants, their roles in woody plants, especially wintersweet (Chimonanthus praecox), are largely unclear. In this study, a wintersweet WRKY gene named CpWRKY75 belonging to group IIc was isolated and its characteristics were identified. CpWRKY75 is a nucleus-localized protein, and exhibited no transcriptional activation activity in yeast. CpWRKY75 was highly expressed in flowers at different bloom stages. Ectopic expression of CpWRKY75 significantly promoted the flowering time of transgenic Arabidopsis (Arabidopsis thaliana), as determined by the rosette leaf number and first flower open time. The expression levels of flowering-related genes were quantified by qRT-PCR, and the results suggested that CpWRKY75 had obvious influence on the expression level of MICRORNA156C (MIR156C), SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3 (SPL3) and SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9 (SPL9), FLOWERING LOCUS T (FT), LEAFY (LFY), SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1), APETALA1 (AP1), CAULIFLOWER (CAL), and FRUITFULL (FUL). These results suggest that CpWRKY75 might have a flowering time regulation function, and additionally provide a new gene resource for the genetic engineering of woody flowering plants.

TRANSITION FROM TRADITIONAL COB CONSTRUCTION TO 3D PRINTING OF CLAY HOMES

ABSTRACT 3D printing of cementitious material can provide an affordable, sustainable, and optimized approach for the construction of homes, without compromising quality or craftsmanship. While most of the current research and development efforts in this field are focused on cement-based concrete printing, this paper focuses on the current state-of-the-art literature review of designing and developing a sustainable clay-based mixture design that mainly includes clay, sand, straw, lime, and water. The goal of this paper is to bridge the gap between typical traditional earth construction, specifically cob construction, and emerging 3D printing of cementitious materials. The specific objective of this paper is to offer some possible changes in the typical cob mixture so that it can be used for 3D printing of clay-based mixtures with sufficient flowability, buildability, strength, and open time (i.e., the time period between printing of one layer and printing of another layer deposited on a layer below). The paper describes typical clay-based mixtures and their traditional process and then specifies the challenges in going from traditional cob construction to advanced computer-controlled robotic 3D printing.

Leveraging range joins for the computation of overlap joins

Joins are essential and potentially expensive operations in database management systems. When data is associated with time periods, joins commonly include predicates that require pairs of argument tuples to overlap in order to qualify for the result. Our goal is to enable built-in systems support for such joins. In particular, we present an approach where overlap joins are formulated as unions of range joins, which are more general purpose joins compared to overlap joins, i.e., are useful in their own right, and are supported well by B+-trees. The approach is sufficiently flexible that it also supports joins with additional equality predicates, as well as open, closed, and half-open time periods over discrete and continuous domains, thus offering both generality and simplicity, which is important in a system setting. We provide both a stand-alone solution that performs on par with the state-of-the-art and a DBMS embedded solution that is able to exploit standard indexing and clearly outperforms existing DBMS solutions that depend on specialized indexing techniques. We offer both analytical and empirical evaluations of the proposals. The empirical study includes comparisons with pertinent existing proposals and offers detailed insight into the performance characteristics of the proposals.

Heat Release during 3d-Printable Materials Setting and Hardening

The paper presents the experimental data on the cement effect typeon the effects of heat generation during the 3D-printable cement materials’setting and hardening. Materials made on the basis of cements CEMI 42.5 RandCEMI 52.5 R, differing in phase contentC3Ain combination with viscosity modifiers of various compositions,have been studied. To control setting kinetics, a penetrometer test was used, hardening kinetics was evaluated by testing the samples for compressive strength after 1, 3, 7, 14, 28 days of hardening. It was found that the useof CEMI 52.5 Rhigh in compositionC3Acauses a significant heating of the mixture already after its setting, which is not observed when using ordinary CEMI 42.5R. The combination of a highly active aluminosilicate modifier with high-strength cement causes a technologically unacceptable reduction in the setting time and open time of mixtures.

Ecological apocalypticism in Ella Hickson's Oil, Lucy Kirkwood's The Children and Duncan Macmillan's Lungs

The vast spatio-temporal dimensions of global warming and ecological devastation are hyberobjects (Morton, 2013) which humans are able to compute but not directly see. This is problematic for dramatists and theatre makers whose work tends to focus on portraying more immediate emotions on a human scale. Diamond (2016) has written insightfully about a number of contemporary British plays which attempt to grapple with the “genre-bending” hyperobject of global warming, either by working within the limitations of conventional genres or by extending the boundaries of those genres. In this essay, I develop her theme by asking how dramaturgical form rather than genre may be harnessed in this task. I examine how Kirkwood exploits the naturalistic form in her play The Children to show the impacts of the hyperobject on the characters’ lives. I further examine how the open time, open place structure (pace Jeffreys, 2019) of Hickson’s reconfigured Brechtian epic Oil enables the playwright to connect human and ecological timescales. I explore how Macmillan has his dramaturgical cake and eats it by pioneering, in his play Lungs, a hybridised form which offers a highly affective audience experience, aligning human and more-than-human timescales. Finally, I theorise that playwrights and theatre makers might profitably experiment with such formal hybridisations in future attempts to dramatize the hyperobject’s ontological vastness.

INVESTIGATION OF THE EFFECTS OF DIFFERENT RETROGRESSION AND RE-AGING PARAMETERS APPLIED TO THE 7075 ALLOY ON THE MICRO-ARC OXIDATION PROCESS

In this paper, retrogression and re-aging (RRA) heat treatment was applied to 7075 aluminum alloy in T6 condition at different times (30 and 90[Formula: see text]min) and temperatures (180∘C and 240∘C). While RRA heat treatments increase the corrosion resistance of the material, it does not harm its mechanical properties. On the other hand, the surface resistance of aluminum is low. Surface modifications are applied to overcome this deficiency. Among these, the micro-arc oxidation (MAO) method increases the corrosion resistance and attains excellent values in surface hardness. To better understand the RRA/MAO relationship, heat-treated (RRA) samples with four different parameters were coated with the MAO method. In this way, a ceramic oxide coating layer was created on the material surfaces. In order to determine the RRA parameter effect, the MAO process parameters are kept constant (anode voltage ([Formula: see text]): 500[Formula: see text]V, cathode voltage ([Formula: see text]): 300[Formula: see text]V, anode voltage open time ([Formula: see text]: 300[Formula: see text][Formula: see text]s, cathode voltage open time ([Formula: see text]): 200[Formula: see text][Formula: see text]s, frequency: 160 coating with Hz, and process time: 20[Formula: see text]min). Surface properties (coating thickness, surface roughness, surface arc duct’s structure, etc.), phase analysis (X-ray diffraction (XRD)) and microstructures (coating cross-section studies: distance-dependent hardness, coating/backing material interface character, coating porosity ratio) were examined. XRD analysis showed that the main phase of the coatings is [Formula: see text]-Al2O3. A coating layer of around 125[Formula: see text][Formula: see text]m was achieved with the growth rate of 6[Formula: see text][Formula: see text]m/min. Surface roughness was between 5.5[Formula: see text][Formula: see text]m and 8[Formula: see text][Formula: see text]m for different RRA parameters. RRA/MAO relation with the characterization made was detailed, and predictions were made for the surface properties of the material (hardness, corrosion resistance, wear, etc.).

Formal Verification of Control and Operational Behaviors in Industrial Internet of Things Services Composition

Industrial Internet of Things (IIoT) services composition rely on composing existing IoT services in industrial context in order to obtain an overall service with added value. The composite service’s behavior is extremely influenced by the time, availability, and accuracy of the unitary services. Thus, it is extremely important to guarantee a provision of IIoT services as expected during the modeling phase. For this, we lie on formal verification to check all the possible scenarios before to pass through IIoT services provision. We propose first to model each process involved in the composition by open Time Petri Nets. These nets offer interface places for the purpose of process communication with the other processes. Then the composition of open Time Petri Nets is guaranteed via superimposing the interface places and thus obtaining a Time Petri Net modeling the composite process. Finally, control and operational behaviors of IIoT services composition formally checked after being specified in an expressive fragment of TCTL temporal logic