Amniotic fluid follicle-stimulating hormone and fetal sex determination in the second trimester of pregnancy

Ultrasound examination of the fetal perineal area was done in third trimester ofpregnancy to determine the fetal sex. We carried out ultrasound on 621 consecutiveobstetrics patients who attended these centers for obstetric causes referred by theirphysicians. Of them 612 had singleton pregnancy and 9 had twin pregnancy. Weattempted to determine the sex of all fetuses (n=630) based on demonstration of maleand female genitalia. In 585 pregnancies, fetal genitalia were well visualized – theaccuracy rate was thus 92.9%, while the rest 7.1% (n=45) could not be determinedwhich was limited by fetal presentation, position, volume of amniotic fluid and colonicgas. Among the correctly determined cases 384 (65.6%) were male and the rest 201(34.4%) were female. About 91% of the mother desired a male child in contrast toonly 3.1% of the mother who desired a female child prior to ultrasound examination.Interesting enough mothers welcoming female child were all multigravida with previousmale child/children. It needs to mention here that no primae mother welcomed femalechild. Some other aspects of prenatal sex determination have also been discussed inthisarticle.Key Words: Ultrasound, Fetal sex, Bangladesh

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INTRAUTERINE FETAL SEX DETERMINATION BY RADIOIMMUNOASSAY OF AMNIOTIC FLUID TESTOSTERONE

Obstetrical & Gynecological Survey ◽

10.1097/00006254-197511000-00005 ◽

1975 ◽

Vol 30 (11) ◽

pp. 731

Author(s):

HARLAN R. GILES ◽

CHARLES D. LOX ◽

M. WAYNE HEINE ◽

C. D. CHRISTIAN

Keyword(s):

Amniotic Fluid ◽

Sex Determination ◽

Fetal Sex ◽

Amniotic Fluid Testosterone

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Response of Superovulation by Using FSH (Follicle Stimulating Hormone) and Sex Determination of Embryos Using PCR in Pesisir Cows of West Sumatra

ANIMAL PRODUCTION ◽

10.20884/1.jap.2018.20.1.630 ◽

2019 ◽

Vol 20 (1) ◽

pp. 7

Author(s):

Tinda Afriani ◽

Fery Lismanto Sayful ◽

Sumedi Sumedi ◽

Dino Eka Putra ◽

Endang Purwati

Keyword(s):

Drug Release ◽

Sex Ratio ◽

Sex Determination ◽

Corpus Luteum ◽

Follicle Stimulating Hormone ◽

West Sumatra ◽

Natural Mating ◽

Stimulating Hormone

This study was conducted to determine the response of superovulation by giving 16 ml dosage of FSH hormone to female Pesisir cattle. The estrus schedule of 15 Pesisir cows was set by inserting CIDR (Controlled Internal Drug Release) into the vagina for 12 days. At day 10, all cattles were injected with FSH for three consequent days but with decreasing dosage. On the 3rd day, FSH injection was accompanied by PGF2α injection and CIDR was removed. The detection of estrus was performed at day 13. Natural mating was proceeded after the estrus signs visible. Collection of donor embryos was done on the 6th and 8th day after mating. The variables measured were the response of superovulation, total number of corpus luteum, number of embryos and sex ratio. The results obtained were all Pesisir cows responded to superovulation. The average number of of corpus luteum and embryoes per cow were 5.93±3.17 and -----, respectively, while the total of transferable embryoes were 90, with an average of 6.00 or 61.64%. The sexing of embryoes obtained in this study from 146 embryoes awere 76.03% males (111 embryoes) and 23.97% females (35 embryoes). Based on total of transferable embryoes, there were 51.37% male embryos and 11.28% of females embryos. The result of this study showed that the sex ratio of male embryos was higher than female embryos.

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Intra-uterine Diagnosis of Fetal Sex

PEDIATRICS ◽

10.1542/peds.48.1.159 ◽

1971 ◽

Vol 48 (1) ◽

pp. 159-160

Author(s):

A. Greensher ◽

R. Gersh ◽

D. Peakman ◽

A. Robinson

Keyword(s):

Pregnant Woman ◽

Muscular Dystrophy ◽

Amniotic Fluid ◽

Y Chromosome ◽

Accurate Method ◽

Clinical Genetics ◽

Second Trimester ◽

Fetal Sex ◽

Interphase Cells ◽

Fluorescent Stains

The ability to examine amniotic fluid obtained by amniocentesis during the second trimester of pregnancy is a significant new advance in the practice of clinical genetics.1 We here report a rapid and accurate method for determining fetal sex, which should be useful when the pregnant woman is a carrier of a serious sex-linked disease such as muscular dystrophy or hemophilia. The method makes use of the selective concentration of fluorescent stains by the Y chromosome in interphase cells.2,3 Recently two observers have reported their preliminary observations on amniotic fluid cells using fluorescent techniques.4,5

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