Three-Dimensional sp2 Carbon-Linked Covalent Organic Frameworks for Bioresponsive Fluorescence Imaging

<a>Three-dimensional (3D) covalent organic frameworks (COFs) are crystalline porous polymers with potential in numerous high-tech applications, but the linkages involved in their synthesis are still rather limited. Herein we report the first </a><a>case of 3D sp² carbon-linked COFs</a> fabricated by the formation reaction of C=C bonds and their application in bioresponsive fluorescence imaging. These new COFs, namely JUC-580 and JUC-581, showed high stability and excellent light-emitting properties in solid state and dispersed in various solvents. <a>Furthermore, we investigated </a>the potential application of JUC-581 for a drug carrier combined with bioresponsive fluorescence imaging. The results indicated that 3D sp² carbon-linked COFs are not only potential drug-loaded and sustained release materials but also promising cell fluorescent stains. This study expands the structural categories of 3D COFs based on different linkages, and promotes their prospective applications for biomedicine and fluorescent materials.

Three-Dimensional sp2 Carbon-Linked Covalent Organic Frameworks for Bioresponsive Fluorescence Imaging

<a>Three-dimensional (3D) covalent organic frameworks (COFs) are crystalline porous polymers with potential in numerous high-tech applications, but the linkages involved in their synthesis are still rather limited. Herein we report the first </a><a>case of 3D sp² carbon-linked COFs</a> fabricated by the formation reaction of C=C bonds and their application in bioresponsive fluorescence imaging. These new COFs, namely JUC-580 and JUC-581, showed high stability and excellent light-emitting properties in solid state and dispersed in various solvents. <a>Furthermore, we investigated </a>the potential application of JUC-581 for a drug carrier combined with bioresponsive fluorescence imaging. The results indicated that 3D sp² carbon-linked COFs are not only potential drug-loaded and sustained release materials but also promising cell fluorescent stains. This study expands the structural categories of 3D COFs based on different linkages, and promotes their prospective applications for biomedicine and fluorescent materials.

Effect of Epigallocatechin Gallate on Viability of Kudoa septempunctata

Kudoa septempunctata have been reported as a causative agent for acute transient gastrointestinal troubles after eating raw olive flounder (Paralichthys olivaceus). It raised public health concerns and quarantine control in several countries. Quantitative evaluation on viability of K. septempunctata is crucial to develop effective chemotherapeutics against it. A cytometry using fluorescent stains was employed to assess effect of three compounds on viability of K. septempunctata. Epigallocatechin gallate reduced markedly viability of K. septempunctata at 0.5 mM or more, and damaged K. septempunctata spores by producing cracks.

Study of effectiveness of antibacterial therapy by the micro-spectral analysis method

The method of fluorescent spectral analysis using the metachromatic fluorescent «Stains all» dye allows finding out the ratio of nucleic acid and proteins in the epithelium of the mucous membrane ferruterous stomach in the control group and infected with Klebsiella pneumoniae. The dynamics of changes in these ratios reflects the special aspects of the functional state of the cells in this zone normally, with klebsiellez disease and in its treatment. In the control group, it stayed within the gradual increase in their functional activity respectively to the increase in the age of the chickens. The changes of functional status of these cells in the infected chickens were complicated, depending on both the impact of the agent and from the action of the “Enroflon” drug. These findings can be taken into account in the formation of a fundamentally new approach to the question of the creation of modern technologies of prevention and treatment of klebsiellez.

Initial Attachment and Biofilm Formation of a Novel Crenarchaeote on Mineral Sulfides

This study focused on colonization and biofilm formation of a new crenarchaeoteAcidianussp. DSM 29099 on pyrite and chalcopyrite. Confocal laser scanning microscopy (CLSM) in combination with several fluorescent stains was applied to examine spatial distribution of cells and biofilms, as well as extracellular polymeric substances (EPS) production on the substrates. Around 60% and 35% of the inoculum adhered to pyrite and chalcopyrite within 2 h, respectively. Cells ofAcidianussp. DSM 29099 were heterogeneously distributed on both pyrite and chalcopyrite surfaces, while large mineral surfaces remained uncolonized. Biofilm cells on pyrite were often found to be embeded in EPS. EPS residues like mannose and glucose were possibly involved in intial attachment to pyrite. A mature biofilm on pyrite was developed after 2-4 days of incubation.