Significant divergence in nucleotide sequences for β-tubulin from different laboratory strains of Chinese hamster ovary cells

DNA Sequence ◽  
1996 ◽  
Vol 6 (3) ◽  
pp. 171-174 ◽  
Author(s):  
Barbara A. Boggs ◽  
Manuel L. Conzalez-Garay ◽  
Fernando Cabral
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Nucleotide Sequences ◽  
Ovary Cells ◽  
Significant Divergence ◽  
Β Tubulin

Cloning of 11 α-tubulin gene sequences from the genome of Chinese hamster ovary cells

1985 ◽  
Vol 63 (6) ◽  
pp. 511-518 ◽  
Author(s):  
Elizabeth M. Elliott ◽  
Farida Sarangi ◽  
Graham Henderson ◽  
Victor Ling
Keyword(s):  
Chinese Hamster Ovary ◽  
Genomic Library ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Coding Region ◽  
Tubulin Gene ◽  
Ovary Cells ◽  
Tubulin Genes ◽  
Clonal Lines ◽  
Β Tubulin

We have analyzed the complex tubulin gene family in clonal lines of Chinese hamster ovary cells. There are approximately 16 α-tubulin genes and a similar multiplicity of β-tubulin genes. The α-tubulin genes are not closely linked to each other nor to the β-tubulin genes. A genomic library has been constructed in the vector λ charon 4A containing insert sizes of 13–20 kilobases. The library has been screened with both inter- and intra-species α-tubulin probes. Eleven α-tubulin clones with different restriction patterns have been isolated and characterized. At least seven of these clones contain the complete gene coding region. One clone appears to represent the transcribed α-tubulin gene II. The sequence of an intron from this gene is compared with that from an equivalent gene in the rat.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells

Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein

2020 ◽  
Vol 17 ◽  
Author(s):  
Shazid Md. Sharker ◽  
Md. Atiqur Rahman
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mass Production ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Therapeutic Protein ◽  
Specific Productivity ◽  
Ovary Cells ◽  
Further Development ◽  
Interesting Area

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

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