Diploid and triploid interspecific hybrids were produced from crosses between four Hordeum vulgare varieties and two diploid and two tetraploid Hordeum bulbosum genotypes. These were studied cytologically using a C-banding technique as well as with conventional staining procedures. C-banding of mitotic preparations of the hybrids enabled all H. vulgare chromosomes to be identified individually, although only the satellited chromosome of H. bulbosum could be distinguished from the others of this genome. Most diploid hybrids had stable somatic chromosome constitutions (2n = 14), although 4 out of 39 plants had a mosaic constitution. Chromosome counts of triploid hybrids, however, revealed that about 40% of plants had a mosaic somatic constitution ranging from 7–22 chromosomes per cell, although 21-chromosome cells were at the highest frequency. Studies of meiosis in diploid hybrids showed that the frequency of pairing between H. vulgare and H. bulbosum chromosomes varied between different cross combinations and appeared to be mainly under the control of the H. bulbosum genome. C-banding revealed that H. vulgare chromosome 6 paired with the satellited chromosome of H. bulbosum. However, this latter chromosome was also absent in the majority of aneuploid cells and appeared to be the first to be eliminated. Meiosis in triploid hybrids was characterized by the presence of univalents, bivalents, and trivalents and, infrequently, higher order associations. Bivalents were formed mainly from pairing between H. bulbosum chromosomes alone, although trivalents were formed from pairing between two H. bulbosum and one H. vulgare chromosomes. All univalents, in cells that contained less than seven, were H. vulgare chromosomes. Overall, these results indicate that a high frequency of homoeologous allosyndesis can occur between the chromosomes of these two species, but it does depend on the H. bulbosum genotype used. It should be possible to introgress genes into H. vulgare through the use of H. bulbosum genotypes that have low frequencies of elimination and high chromosome pairing.Key words: Hordeum vulgare, H. bulbosum, C-banding, chromosome pairing.