Demonstration of Sensory-Motor Innervation of Postmetamorphic Forelimb Regenerates ofTriturus Alpestris(Urodela) on Cryotome Serial Sections Using Horseradish Peroxidase

1990 ◽  
Vol 65 (3) ◽  
pp. 119-123
Author(s):  
Hermann Josef Anton ◽  
Axel Oberemm
Keyword(s):  
Horseradish Peroxidase ◽  
Serial Sections ◽  
Motor Innervation ◽  
Sensory Motor

scholarly journals LOCALIZATION OF α-FETOPROTEIN IN HUMAN AND RAT FETAL LIVERS AN IMMUNOHISTOCHEMICAL METHOD USING HORSERADISH PEROXIDASE

1974 ◽  
Vol 22 (6) ◽  
pp. 414-418 ◽  
Author(s):  
N. C. NAYAK ◽  
P. K. DAS ◽  
U. N. BHUYAN ◽  
ASHA MITTAL
Keyword(s):  
Connective Tissue ◽  
Horseradish Peroxidase ◽  
Immunohistochemical Method ◽  
Immunoperoxidase Technique ◽  
Serial Sections ◽  
Immunofluorescence Method ◽  
Immunohistochemical Technique

α-Fetoprotein (AFP) was successfully demonstrated in paraffin-embedded sections of human and rat fetal livers, by a multilayering immunohistochemical technique using the peroxidase-antiperoxidase system. Serial sections simultaneously subjected to immunofluorescence showed identical sites of localization in hepatocytic cytoplasm and sometimes in perivenous connective tissue. AFP-containing hepatocytes were located around efferent veins as well as randomly in the lobule. The immunoperoxidase technique has certain advantages over the immunofluorescence method which would justify the former's application in studies on the dynamics of AFP synthesis.

The motor innervation of the single-bellied digastric muscle in the rabbit: A retrograde horseradish peroxidase study

1985 ◽  
Vol 56 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Ronald H. Baisden ◽  
Michael L. Woodruff ◽  
Dennis L. Whittington ◽  
Amy E. Benson
Keyword(s):  
Horseradish Peroxidase ◽  
Digastric Muscle ◽  
Motor Innervation

Selective bilateral motor innervation in Xenopus tadpoles with one hind limb

Development ◽  
1981 ◽  
Vol 65 (1) ◽  
pp. 149-163
Author(s):  
Alan H. Lamb
Keyword(s):  
Horseradish Peroxidase ◽  
Hind Limb ◽  
Limb Bud ◽  
The Other ◽  
Motor Innervation ◽  
Two Sides

Bilateral innervation of a single hindlimb bud was induced by amputating the other limb bud and disrupting the barriers between the two sides. Though the routes of the crossed nerves were necessarily abnormal, the motor projections that developed subsequently were normal as determined by horseradish peroxidase tracing. The limb therefore appears to be innervated selectively, each region being invaded and/or synapsed with only by motoneurones at particular locations. The numbers of motoneurones surviving after metamorphosis were almost normal on both sides provided the operation was done before motor invasion of the limb bud begins. From this it is argued that the axons were probably guided actively to their correct destinations. Without such guidance, axons would probably not have been able to find their correct termination sites and motoneurone survival would therefore have been depressed. The normal motoneurone numbers also imply that the single limb was supporting twice its usual quota of motoneurones. The hypothesis that motoneurones compete in the limb for survival is therefore not supported.

Further experiments on the innervation and function of grafted supernumerary limbs in the embryo of Eleutherodactylus martinicensis

Development ◽  
1964 ◽  
Vol 12 (2) ◽  
pp. 229-245
Author(s):  
Arthur Hughes
Keyword(s):  
Serial Sections ◽  
Motor Innervation ◽  
Motor Nerves ◽  
Supernumerary Limbs ◽  
And Function

Previous studies on the transplantation of limbs in the embryo of Eleutherodactylus martinicensis have shown that supernumerary limbs tend to be less well innervated than are corresponding replacing grafts, and that the innervation of the former, either autografts or homografts, is progressively less extensive with increase in time after operation. The degree to which grafted supernumerary forelimbs show movement in life parallels the extent of their motor innervation when studied subsequently in serial sections. The loss of innervation and of movement is particularly drastic near the time when the animal normally hatches and the tail undergoes atrophy. The present paper describes some further experiments made with the aim of throwing light on the causes which underly the degeneration of motor nerves within supernumerary limb grafts in this animal. The source of the embryos of Eleutherodactylus martinicensis, the methods of culturing them, and of limb grafting, remain the same as in other papers on this subject (Hughes, 1962, 1964a, 1964b).

The pattern of innervation in serially duplicated axolotl limbs: further evidence for the existence of local pathway cues?

Development ◽  
1987 ◽  
Vol 100 (3) ◽  
pp. 479-487
Author(s):  
N. Stephens ◽  
N. Holder
Keyword(s):  
Spinal Cord ◽  
Horseradish Peroxidase ◽  
Local Environment ◽  
Retrograde Transport ◽  
Limb Muscle ◽  
Biceps Muscle ◽  
Motor Innervation ◽  
Nerve Sprouting ◽  
Correct Pattern ◽  
Motor Neurones

The innervation of the biceps muscle was examined in regenerated and vitamin A-induced serially duplicated axolotl forelimbs using retrograde transport of horseradish peroxidase. The regenerated biceps muscle becomes innervated by motor neurones in the same position in the spinal cord as the normal biceps motor pool. In previous experiments in which the innervation of a second copy of a proximal limb muscle was examined in serially duplicated limbs (Stephens, Holder & Maden, 1985), the duplicate muscle was found to become innervated by motor neurones that would normally have innervated distal muscles. In the present study, the innervation of the second copy of biceps was examined under conditions designed to encourage nerve sprouting from ‘correct’ biceps axons. Following either partial limb denervation or denervation coupled with removal of the proximal biceps, the second copy of the muscle was still innervated by inappropriate motor neurones, which again would normally innervate distal limb muscles. These results are interpreted as evidence for the necessity for an appropriate local environment for axonal growth to allow reformation of a correct pattern of motor innervation in the regenerated limb.

Motor innervation of proximally rotated chick embryo wings

Development ◽  
1984 ◽  
Vol 83 (1) ◽  
pp. 213-223
Author(s):  
N. G. Laing
Keyword(s):  
Spinal Cord ◽  
Electrical Stimulation ◽  
Chick Embryo ◽  
Horseradish Peroxidase ◽  
Brachial Plexus ◽  
Limb Bud ◽  
Motor Innervation ◽  
Lateral Edge ◽  
Limb Innervation

Chick embryo wing buds were rotated close to the lateral edge of the somites at stage 19, prior to limb innervation. Despite the abnormal orientation of the resulting limb, the motor pools to biceps and triceps were largely normal, as judged by electrical stimulation and horseradish peroxidase labelling just prior to hatching. The only abnormalities were a few caudal motoneurons innervating biceps and a few rostral motoneurons innervating triceps. This distribution is similar to that seen normally in young embryos before the completion of motoneuron death and it is suggested that the rotation may be keeping alive motoneurons which otherwise would die. The morphology of the brachial plexus supplying rotated wings was abnormal. It is concluded that axons growing into the limb bud from the spinal cord can compensate for reversal of both the limb axes and selectively innervate appropriate muscles. The result is consistent with others in which proximal reversal of one limb axis alone produced normal innervation.

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