scholarly journals In vitro inoculation effects and colonization pattern of Leohumicola verrucosa, Oidiodendron maius, and Leptobacillium leptobactrum on fibrous and pioneer roots of Vaccinium oldhamii hypocotyl cuttings

Plant Root ◽  
2021 ◽  
Vol 15 (0) ◽  
pp. 1-9
Author(s):  
Takashi Baba ◽  
Dai Hirose ◽  
Takuya Ban
Keyword(s):  
Colonization Pattern ◽  
Oidiodendron Maius ◽  
Hypocotyl Cuttings

Initiation of roots on hypocotyl cuttings of Pinussylvestris, with emphasis on direct rooting, root elongation, and auxin uptake

1988 ◽  
Vol 18 (11) ◽  
pp. 1457-1462 ◽  
Author(s):  
Roland Grönroos ◽  
Sara von Arnold
Keyword(s):  
In Vitro Culture ◽  
Nutrient Solution ◽  
Acid Treatment ◽  
Root Elongation ◽  
Hydroponic Culture ◽  
Indolylacetic Acid ◽  
Auxin Uptake ◽  
Hypocotyl Cuttings ◽  
Wound Tissue

Direct rooting of hypocotyl cuttings of Pinussylvestris L. is described and compared with rooting via wound tissue. The optimal 3-indolylbutyric acid treatment for direct rooting of Pinussylvestris hypocotyl cuttings under in vitro conditions was 1.23 mM for 24 h. This treatment resulted in 45% rooting within 3 weeks after cutting. During this period, only direct rooting was observed. Three to 4 weeks after cutting, roots developed either directly on the hypocotyl or via wound tissue. After the first month, rooting took place predominantly via wound tissue. The optimal 3-indolylbutyric acid treatment found for in vitro culture could successfully be used for hydroponic culture (under nonsterile conditions). In vitro culture inhibited root elongation. However, after transfer of rooted cuttings from in vitro to hydroponic culture, root elongation increased markedly. Uptake studies with 3-[5(n)-3H]indolylacetic acid mixed with the nutrient solution showed that auxin uptake by the cutting probably occurred mainly through uptake of the nutrient solution. After 24 h treatment with 1.43 mM 3-indolylacetic acid, the concentration taken up by the cuttings was 0.91 nmol/mg fresh weight. Differences between individual cuttings in auxin uptake could not explain the poor direct rooting of Pinussylvestris hypocotyl cuttings cultured in vitro.

scholarly journals In Vitro Rooting of Hypocotyl Cuttings of Fraser Fir

HortScience ◽  
1990 ◽  
Vol 25 (12) ◽  
pp. 1650-1651 ◽  
Author(s):  
Carole H. Saravitz ◽  
Frank A. Blazich ◽  
Henry V. Amerson
Keyword(s):  
Butyric Acid ◽  
In Vitro Rooting ◽  
Rooted Cutting ◽  
Abies Fraseri ◽  
Fraser Fir ◽  
Hypocotyl Cuttings

Hypocotyl cuttings were prepared from Ii-week-old aseptically grown seedlings of Fraser fir [Abies fraseri (Pursh) Poir.] and cultured 18 days on media containing 0 to 40 mg IBA/liter followed by transfer to the same medium without auxin. Greatest rooting (66%) occurred after treatment with 20 mg IBA/liter, whereas the greatest number of roots per rooted cutting (7.4) was noted following treatment with 40 mg·liter-1. Chemical name used: 1H-indole-3-butyric acid (IBA).

Growth analyses on in vitro, ex vitro and auxin-rooted hypocotyl cuttings of Pinus contorta Dougl. ex Loud.

1993 ◽  
Vol 125 (4) ◽  
pp. 829-836 ◽  
Author(s):  
ROLAND GRONROOS ◽  
GUNNAR FLYGH ◽  
MONIKA KAHR ◽  
SARA ARNOLD
Keyword(s):  
Pinus Contorta ◽  
Ex Vitro ◽  
Hypocotyl Cuttings ◽  
Growth Analyses

Initiation of roots on hypocotyl cuttings of Pinus contorta in vitro

1987 ◽  
Vol 69 (2) ◽  
pp. 227-236 ◽  
Author(s):  
Roland Gronroos ◽  
Sara Arnold
Keyword(s):  
Pinus Contorta ◽  
Hypocotyl Cuttings

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

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