In order to explore the distribution and the loss way of inorganic substances in bone and to provide scientific basis for prevention and therapy of osteoporosis, Proton Induced X-ray Emission (PIXE) method is used for the determination of elemental concentration in femoral head from five autopsies and seven patients with femoral neck broken, and Synchrotron Radiation X-ray Fluorescence (SRXRF) microprobe analysis technique is used to scan a slice of the femoral head from its periphery to its center, via cartilage, compact and spongy zones. The results show that the concentrations of P , Ca , Fe , Cu , Sr in a control group are higher than those in a patient group, but the concentrations of S , K , Zn , Mn are not significantly different. The quantitative computerized tomography of elemental distribution, such as Ca , P , K , Fe , Zn , Sr and Pb in bone slice tissue including cartilage, substantial compact and substantial spongy, is investigated. Combined with the correlations between P , K , Zn , Sr and Ca , the loss way of minerals and the physiological functions of some metal elements in bone are also discussed.
Methods for determination of inorganic substances in water and fluvial sediments