High Temperature Laser-Scanning Confocal Microscopy for thein-situInvestigation of Grain Growth and Phase Transformations in Intermetallic γ-TiAl based Alloys

One of the classic limitations of light microscopy has been the fact that three dimensional biological events could only be visualized in two dimensions. Recently, this shortcoming has been overcome by combining the technologies of laser scanning confocal microscopy (LSCM) and computer processing of microscopical data by volume rendering methods. We have employed these techniques to examine morphogenetic events characterizing early development of sea urchin embryos. Specifically, the fourth cleavage division was examined because it is at this point that the first morphological signs of cell differentiation appear, manifested in the production of macromeres and micromeres by unequally dividing vegetal blastomeres.The mitotic spindle within vegetal blastomeres undergoing unequal cleavage are highly polarized and develop specialized, flattened asters toward the micromere pole. In order to reconstruct the three-dimensional features of these spindles, both isolated spindles and intact, extracted embryos were fluorescently labeled with antibodies directed against either centrosomes or tubulin.

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Observation of Fine Structure in Bicontinuous Phase-Separated Domains of a Polymer Blend by Laser Scanning Confocal Microscopy

Macromolecules ◽

10.1021/ma010190d ◽

2001 ◽

Vol 34 (15) ◽

pp. 5186-5191 ◽

Cited By ~ 11

Author(s):

Hiroshi Jinnai ◽

Hiroshi Yoshida ◽

Kohtaro Kimishima ◽

Yoshinori Funaki ◽

Yoshitsugu Hirokawa ◽

...

Keyword(s):

Fine Structure ◽

Confocal Microscopy ◽

Polymer Blend ◽

Laser Scanning ◽

Laser Scanning Confocal Microscopy ◽

Scanning Confocal Microscopy

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The DNA content of trophozoites and cysts of Giardia lamblia by microdensitometric quantitation of Feulgen staining and examination by laser scanning confocal microscopy.

Journal of Histochemistry & Cytochemistry ◽

10.1177/42.11.7930524 ◽

1994 ◽

Vol 42 (11) ◽

pp. 1413-1416 ◽

Cited By ~ 24

Author(s):

S L Erlandsen ◽

E M Rasch

Keyword(s):

Confocal Microscopy ◽

Genome Size ◽

Dna Content ◽

Giardia Lamblia ◽

Laser Scanning ◽

Laser Scanning Confocal Microscopy ◽

Evolutionary Development ◽

Scanning Confocal Microscopy ◽

Dividing Cells ◽

C Value

We investigated direct measurement of the DNA content of the parasitic intestinal flagellate Giardia lamblia through quantitation by Feulgen microspectrophotometry and also by visualization of Feulgen-stained DNA chromosomes within dividing cells by laser scanning confocal microscopy. Individual trophozoites of Giardia (binucleate) contained 0.144 +/- 0.018 pg of DNA/cell or 0.072 pg DNA/nucleus. Giardia lamblia cysts (quadranucleate) contained 0.313 +/- 0.003 pg DNA or 0.078 pg DNA/nucleus. The genome size (C) value per nucleus ranged between 6.5-7.1 x 10(7) BP for trophozoites and cysts, respectively. Confocal microscopic examination of Giardia trophozoites undergoing binary fission revealed five chromosome-like bodies within each nucleus. Further information about genome size and DNA content within different Giardia species may help to clarify the pivotal role of these primitive eukaryotic cells in evolutionary development.

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