In-situ Observation of Austenite Grain Growth in Plain Carbon Steels by Means of High-temperature Laser Scanning Confocal Microscopy

ABSTRACTIn-situ observation of the formation and disappearance of the surface relief associated with the twinning during the order-disorder transitions among CuAu-I (L10), CuAu-II (PAP) and disordered fcc phases was conducted using Confocal Scanning Laser Microscopy equipped with a gold image furnace. The Retro effect was confirmed in poly-crystal samples, however no evidence was found in single-crystal samples. Also observed in poly-crystal samples are that the disordering temperature detected by the disappearing of relieves is different from grain to grain, and that grain boundary cracking alleviates the Retro effect. The observed phenomena were explained based on the crystallographic orientation relationship among grains investigated by FESEM/EBSD in terms of the elastic strain effect around grain boundaries induced by transition. It was confirmed that in each grain the surface relieves correspond to a set of two {011} planes having a <100> axis perpendicular to both planes in common. It was also found that the larger the average strain of two neighboring grains is, the lower the transition temperature. This observation was explained by the stress effect on the stability of a phase.

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Three-Dimensional Laser-Scanning Confocal Microscopy of In Situ Hybridization in the Skin

American Journal of Dermatopathology ◽

10.1097/00000372-199402000-00009 ◽

1994 ◽

Vol 16 (1) ◽

pp. 44-51 ◽

Cited By ~ 3

Author(s):

Stephen E. Mahoney ◽

Stephen W. Paddock ◽

Louis C. Smith ◽

Dorothy E. Lewis ◽

Madeleine Duvic

Keyword(s):

In Situ Hybridization ◽

Confocal Microscopy ◽

Laser Scanning ◽

Three Dimensional ◽

Laser Scanning Confocal Microscopy ◽

Scanning Confocal Microscopy

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High Temperature Laser-Scanning Confocal Microscopy for thein-situInvestigation of Grain Growth and Phase Transformations in Intermetallic γ-TiAl based Alloys

Practical Metallography ◽

10.3139/147.110341 ◽

2015 ◽

Vol 52 (5) ◽

pp. 259-269 ◽

Cited By ~ 3

Author(s):

T. Klein ◽

S. Niknafs ◽

R. Dippenaar ◽

H. Clemens ◽

S. Mayer

Keyword(s):

High Temperature ◽

Confocal Microscopy ◽

Phase Transformations ◽

Grain Growth ◽

Laser Scanning ◽

Laser Scanning Confocal Microscopy ◽

Scanning Confocal Microscopy

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An In Situ Hybridization Histochemistry Technique Allowing Simultaneous Visualization by the Use of Confocal Microscopy of Three Cellular mRNA Species in Individual Neurons

Journal of Histochemistry & Cytochemistry ◽

10.1177/002215549804600608 ◽

1998 ◽

Vol 46 (6) ◽

pp. 753-759 ◽

Cited By ~ 21

Author(s):

Michel Grino ◽

Alfredo J. Zamora

Keyword(s):

Confocal Microscopy ◽

Laser Scanning ◽

Simultaneous Detection ◽

Laser Scanning Confocal Microscopy ◽

Mrna Species ◽

Scanning Confocal Microscopy ◽

Detection Systems ◽

Low Concentrations ◽

Simultaneous Visualization

We present a specific and sensitive method for simultaneous detection of three mRNA species in individual neurons. The method relies on the use of riboprobes labeled with [35S]-UTP, digoxigenin-UTP, or biotin-UTP. The nonradioactive probes were sequentially revealed by incubation with anti-digoxigenin immunoglobulins or streptavidin conjugated to peroxidase, followed by the use of fluorochrome-labeled tyramides as peroxidase substrates. The radioactive probe was revealed by conventional autoradiography. There was no interaction among the different probes or the various detection systems. We demonstrate the use of this method by illustrating on laser scanning confocal microscopy the co-localization of the mRNAs coding for corticotropin-releasing factor (CRF), arginine vasopressin (AVP), or peptidylglycine α-amidating monooxygenase (PAM) in rat hypothalamic paraventricular nucleus (PVN) and its modulation by endogenous glucocorticoids. Our results suggest that this method could be used not only to study the regulation of the hypothalamo-pituitary-adrenal axis but also in various models in which mRNAs are present at low concentrations.

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