scholarly journals Study of the Role of Dopamine Receptors in Streptozotocin-Induced Depressive-Like Behavior Using the Forced Swim Test Model

2017 ◽  
Vol 7 ◽  
pp. e954
Author(s):  
Afshin Roostaei ◽  
Gholamhassan Vaezi ◽  
Mohammad Nasehi ◽  
Ali Haeri-Rohani ◽  
Mohammad-Reza Zarrindast
Keyword(s):  
Receptor Antagonist ◽  
Dopamine Receptors ◽  
Dopamine Receptor ◽  
Forced Swim Test ◽  
Male Rats ◽  
Test Model ◽  
Dopamine Receptor Antagonist ◽  
Swim Test ◽  
Forced Swim

Background: Diabetes is one of the most common endocrine diseases characterized by hyperglycemia. It is caused by an absolute or relative insulin deficiency or an insulin function deficiency. It is one of the major risk factors of depression, with the rate of depression in diabetic patients amounting to as high as 30%. This study examined the role of dopamine receptors in streptozotocin (STZ)-induced depressive-like behavior using the forced swim test (FST). Materials and Methods: This study was performed on 56 Wistar male rats. STZ at doses of 30 and 60 mg/kg body weight was administered via intraperitoneal (IP) route to induce diabetes and depression in rats. Thereafter, by using halobenzazepine (SCH23390) (D1 dopamine receptor antagonist) and sulpiride (D2 receptor dopamine receptor antagonist), the role of dopamine receptors in STZ-induced depression was studied. The one-way analysis of variance technique, Tukey’s range test, and t-test were used to analyze the data. The P-value less than 0.05 was regarded as statistically significant. Results: Our study showed that STZ at doses of 30 and 60 mg/kg, two weeks after injection, caused prolonged immobility in FST, indicating depressive-like behavior (P<0.05 and P<0.01, respectively). SCH23390 (0.001 mg/mL/kg) and sulpiride (0.1mg/mL/kg) did not change the variables of depression in animals that received STZ (at doses of 30 and 60 mg/mL/kg) two weeks before (P>0.05). Conclusion: According to our study, STZ has a depressive-like behavior two weeks after injection, and dopamine receptors do not play a role in depression associated with STZ use. [GMJ.2018;7:e954]

Knockout mice in understanding the mechanism of action of lithium

2009 ◽  
Vol 37 (5) ◽  
pp. 1121-1125 ◽  
Author(s):  
Galila Agam ◽  
Yuly Bersudsky ◽  
Gerard T. Berry ◽  
Diederik Moechars ◽  
Yael Lavi-Avnon ◽  
...  
Keyword(s):  
Knockout Mice ◽  
Glycogen Synthase ◽  
Forced Swim Test ◽  
Test Model ◽  
Swim Test ◽  
Behavioural Analysis ◽  
Behavioural Effects ◽  
Forced Swim ◽  
Increased Sensitivity

Lithium inhibits IMPase (inositol monophosphatase) activity, as well as inositol transporter function. To determine whether one or more of these mechanisms might underlie lithium's behavioural effects, we studied Impa1 (encoding IMPase) and Smit1 (sodium–myo-inositol transporter 1)-knockout mice. In brains of adult homozygous Impa1-knockout mice, IMPase activity was found to be decreased; however, inositol levels were not found to be altered. Behavioural analysis indicated decreased immobility in the forced-swim test as well as a strongly increased sensitivity to pilocarpine-induced seizures. These are behaviours robustly induced by lithium. In homozygous Smit1-knockout mice, free inositol levels were decreased in the frontal cortex and hippocampus. These animals behave like lithium-treated animals in the model of pilocarpine seizures and in the Porsolt forced-swim test model of depression. In contrast with O'Brien et al. [O'Brien, Harper, Jove, Woodgett, Maretto, Piccolo and Klein (2004) J. Neurosci. 24, 6791–6798], we could not confirm that heterozygous Gsk3b (glycogen synthase kinase 3β)-knockout mice exhibit decreased immobility in the Porsolt forced-swim test or decreased amphetamine-induced hyperactivity in a manner mimicking lithium's behavioural effects. These data support the role of inositol-related processes rather than GSK3β in the mechanism of the therapeutic action of lithium.

Antidepressant-like effect of 17β-estradiol: involvement of dopaminergic, serotonergic, and (or) sigma-1 receptor systems

2008 ◽  
Vol 86 (10) ◽  
pp. 726-735 ◽  
Author(s):  
Ashish Dhir ◽  
S.K. Kulkarni
Keyword(s):  
Receptor Antagonist ◽  
Dopamine Receptor ◽  
Receptor Agonist ◽  
Forced Swim Test ◽  
Sigma 1 Receptor ◽  
Dopamine Receptor Antagonist ◽  
Swim Test ◽  
Sigma 1 ◽  
17Β Estradiol ◽  
Immobility Period

17β-estradiol has been reported to possess antidepressant-like activity in animal models of depression, although the mechanism for its effect is not well understood. The present study is an effort in this direction to explore the mechanism of the antidepressant-like effect of 17β-estradiol in a mouse model(s) of behavioral depression (despair behavior). Despair behavior, expressed as helplessness to escape from a situation (immobility period), as in a forced swim test in which the animals are forced to swim for a total of 6 min, was recorded. The antiimmobility effects (antidepressant-like) of 17β-estradiol were compared with those of standard drugs like venlafaxine (16 mg/kg, i.p.). 17β-estradiol produced a U-shaped effect in decreasing the immobility period. It had no effect on locomotor activity of the animal. The antidepressant-like effect was comparable to that of venlafaxine (16 mg/kg, i.p.). 17β-estradiol also exhibited a similar profile of antidepressant action in the tail suspension test. When coadministered with other antidepressant drugs, 17β-estradiol (5 μg/kg, i.p.) potentiated the antiimmobility effect of subeffective doses of fluoxetine (5 mg/kg, i.p.), venlafaxine (2 mg/kg, i.p.), or bupropion (10 mg/kg, i.p.), but not of desipramine (5 mg/kg, i.p.) or tranylcypromine (2 mg/kg, i.p.), in the forced swim test. The reduction in the immobility period elicited by 17β-estradiol (20 μg/kg, i.p.) was reversed by haloperidol (0.5 mg/kg, i.p.; a D2 dopamine receptor antagonist), SCH 23390 (0.5 mg/kg, i.p.; a D1 dopamine receptor antagonist), and sulpiride (5 mg/kg, i.p.; a specific dopamine D2 receptor antagonist). In mice pretreated with (+)-pentazocine (2.5 mg/kg, i.p.; a high-affinity sigma-1 receptor agonist), 17β-estradiol (5 μg/kg, i.p.) produced a synergistic effect. In contrast, pretreatment with progesterone (10 mg/kg, s.c.; a sigma-1 receptor antagonist neurosteroid), rimcazole (5 mg/kg, i.p.; another sigma-1 receptor antagonist), or BD 1047 (1 mg/kg, i.p.; a novel sigma-1 receptor antagonist) reversed the antiimmobility effects of 17β-estradiol (20 μg/kg, i.p.). Similarly, in mice pretreated with a subthreshold dose of 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT, a 5-HT1A serotonin receptor agonist), 17β-estradiol (5 μg/kg, i.p.) produced an antidepressant-like effect. These findings demonstrate that 17β-estradiol exerted an antidepressant-like effect preferentially through the modulation of dopaminergic and serotonergic receptors. This action may also involve the participation of sigma-1 receptors.

The role of hair in swimming of laboratory mice: implications for behavioural studies in animals with abnormal hair

2005 ◽  
Vol 39 (4) ◽  
pp. 370-376 ◽  
Author(s):  
A V Kalueff ◽  
P Tuohimaa
Keyword(s):  
Biomedical Research ◽  
Forced Swim Test ◽  
Laboratory Animals ◽  
Laboratory Mice ◽  
Swim Test ◽  
Forced Swim ◽  
Laboratory Rodents ◽  
Behavioural Research ◽  
Skin Abnormalities

Animal swimming tests, such as the forced swim test, are extensively used in biomedical research to study rodent behaviour. Hair and skin exposed to water may be an important factor affecting the performance in this test. Since various hair and skin abnormalities are not uncommon in genetically modified or drug-treated laboratory animals, this test may be inappropriate for these animals. Because on occasions it is necessary to screen their swimming behaviour, in the present study we aimed to assess the role of hair in swimming of laboratory rodents in the forced swim test, widely used in behavioural research. For this, we shaved laboratory mice (129S1 strain) and compared their swimming patterns with those of unshaven controls. Overall, shaving mice did not affect their swimming behaviours in the 5 min forced swim test. Our results indicate that hair condition is not an important factor in the forced swim test for this mouse strain, and suggest that this test may have wider utility for behavioural analyses of mice with abnormal hair.

scholarly journals The selective glucocorticoid receptor antagonist CORT 108297 decreases neuroendocrine stress responses and immobility in the forced swim test

2014 ◽  
Vol 65 (4) ◽  
pp. 363-371 ◽  
Author(s):  
Matia B. Solomon ◽  
Aynara C. Wulsin ◽  
Taylor Rice ◽  
Dayna Wick ◽  
Brent Myers ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Receptor Antagonist ◽  
Stress Responses ◽  
Forced Swim Test ◽  
Swim Test ◽  
Forced Swim

Antidepressant-like effects of ketamine, norketamine and dehydronorketamine in forced swim test: Role of activity at NMDA receptor

2015 ◽  
Vol 99 ◽  
pp. 301-307 ◽  
Author(s):  
Kinga Sałat ◽  
Agata Siwek ◽  
Gabriela Starowicz ◽  
Tadeusz Librowski ◽  
Gabriel Nowak ◽  
...  
Keyword(s):  
Nmda Receptor ◽  
Forced Swim Test ◽  
Swim Test ◽  
Forced Swim
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