scholarly journals Identification of Aptamers that Specifically Bind to A1 Antigen by Performing Cell-on Human Erythrocytes

2020 ◽  
Vol 9 ◽  
pp. 1657
Author(s):  
Seyed Mohammad Hasan Hosseini ◽  
Mohammad Reza Bassami ◽  
Alireza Haghparast ◽  
Mojtaba Sankian ◽  
Gholamreza Hashemi Tabar
Keyword(s):  
Blood Group ◽  
Dna Aptamer ◽  
Diagnostic Tools ◽  
Blood Group Antigens ◽  
Apparent Dissociation Constant ◽  
Fluorescent Intensity ◽  
Target Molecules ◽  
Polymerase Chain ◽  
Dissociation Constant Kd

Background: The apply of aptamers as a new generation’s way to probe diagnostic for the detection of target molecules has gained ground. Aptamers can be used as alternatives to diagnostic antibodies for detection of blood groups due to their unique features. This study was aimed to produce DNA diagnostic aptamer detecting the antigen of A1 blood group using the Cell-Selex method. Materials and Methods: DNA aptamer was isolated against A1 RBC antigen after ten stages of Cell-Selex and amplification by an asymmetric polymerase chain reaction. The progress of the stages of selection was evaluated using flow cytometry analysis, which the DNA aptamer isolated from the tenth cycle with an affinity of 70% fluorescent intensity, was selected from four positive colonies followed by determination of the sequences and secondary structures. Results: The aptameric sequence obtained from C4 cloning was calculated with the highest binding affinity to A1 antigen having an apparent dissociation constant (Kd value) of at least 29.5 ± 4.3 Pmol, which was introduced as the selected aptamer-based on ΔG obtained from a colony of C4 equal to –13.13. Conclusion: The aptamer obtained from using Cell-Selex method could be used as an example for the development of diagnostic tools such as biosensors for detecting A1 blood group antigens. [GMJ.2020;9:e1657] 

scholarly journals Use of Domain-Deletion Mutants to Locate Lutheran Blood Group Antigens to Each of the Five Immunoglobulin Superfamily Domains of the Lutheran Glycoprotein: Elucidation of the Molecular Basis of the Lua/Lub and the Aua/Aub Polymorphisms

Blood ◽  
1997 ◽  
Vol 89 (11) ◽  
pp. 4219-4225 ◽  
Author(s):  
S.F. Parsons ◽  
G. Mallinson ◽  
G.L. Daniels ◽  
C.A. Green ◽  
J.S. Smythe ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
K562 Cells ◽  
Base Change ◽  
Immunoglobulin Superfamily ◽  
Deletion Mutants ◽  
Blood Group Antigens ◽  
Reading Frame ◽  
Polymerase Chain ◽  
Domain 3

Abstract Lutheran glycoprotein (Lu gp) has five predicted immunoglobulin superfamily (IgSF ) domains. K562 cells were transfected with Lu cDNA and tested by flow cytometry with monoclonal antibodies and Lu blood group antisera. The results confirmed the identity of Lu cDNA. Deletion mutants lacking the regions encoding one or more IgSF domains were made by inverse polymerase chain reaction (PCR), expressed in K562 cells, and tested with the same antibodies. The Lub and Lu5 antigens and the epitope recognized by monoclonal antibody BRIC 224 were mapped to the first, N-terminal, IgSF domain. Lu4 and Lu8 were mapped to domain 2; Lu20 to domain 3; Lu7 and BRIC 221 epitope to domain 4, and Lu13 and Aub to domain 5. The organization of the LU gene was determined. The region encoding the open reading frame is arranged in 15 exons extending over ≈11 kb on chromosome 19q13.2. The Lua/Lub and Aua/Aub blood group polymorphisms were studied using genomic DNA from typed blood donors. The Lua mutation is a base change in exon 3 (G252 to A) encoding an Arg77 (Lub) to His (Lua) change on the CFG face of domain 1. The Aua/Aub polymorphism is an A1637 to G substitution in exon 12 encoding a Thr539 (Aua) to Ala (Aub) change on the G strand of domain 5.

BLOOD GROUP AND HUMAN DISEASES (REVIEW OF LITERATURE)

2020 ◽  
Vol 65 (4) ◽  
pp. 216-221
Author(s):  
Frida Nasyrovna Gilmiyarova ◽  
N. A. Kolotyeva ◽  
V. I. Kuzmicheva ◽  
O. A. Gusyakova ◽  
I. A. Borodina ◽  
...  
Keyword(s):  
Blood Group ◽  
Gastrointestinal Diseases ◽  
Antigenic Determinants ◽  
Blood Group Antigens ◽  
Oral Diseases ◽  
Group Type ◽  
Pathological Conditions ◽  
Definition Of ◽  
Meta Analyses

AB0 blood group antigens were discovered over a century ago; however, it is still important to study their role in development of various pathological conditions. Today it is known that antigenic determinants of this blood group are present not only on erythrocyte membrane but also on other cells and tissues: platelets, gastrointestinal epithelium and salivary glands, respiratory system cells. In the last decade, a large number of studies have appeared to reveal the relationship between a specific disease and blood group type, meta-analyses have been published. Previously, the authors have studied the metabolic status, cell composition and coagulation profile of clinically healthy individuals for more than on 180,000 donations, that allowed to identify group-specific features for each blood group. This review presents generalized data on the association of such pathological conditions as coronary heart disease, thromboembolic complications, tumors of various localizations, inflammatory and destructive oral diseases, psychiatric and some infectious diseases with the presence or absence of antigenic determinants A and B. Carriers of blood group 0 (I) are generally more resistant to diseases, with the exception of H.pylori-associated gastrointestinal diseases. Carriers of «antigenic» blood groups A (II), B (III), AB (IV) are more susceptible to development of infectious, cardiovascular and cancer diseases. The presented data demonstrate clinical significance of the definition of group typing not only for selection of blood and its components during transfusion and transplantation, but also for diagnostics, determination of risk group and tactics for treatment patients with different nosologies.

Determination of homozygote vs heterozygote of Rh blood group antigens via rosette assays

Transfusion ◽  
1982 ◽  
Vol 22 (3) ◽  
pp. 194-196 ◽  
Author(s):  
AB Loren ◽  
Y Matsuo ◽  
D Charman ◽  
MM Yokoyama
Keyword(s):  
Blood Group ◽  
Blood Group Antigens ◽  
Rh Blood Group

Rapid genotyping of blood group antigens by multiplex polymerase chain reaction and DNA microarray hybridization

Transfusion ◽  
2005 ◽  
Vol 45 (5) ◽  
pp. 667-679 ◽  
Author(s):  
Sigrid H.W. Beiboer ◽  
Tinka Wieringa-Jelsma ◽  
Petra A. Maaskant-Van Wijk ◽  
C. Ellen van der Schoot ◽  
Rob van Zwieten ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Group ◽  
Dna Microarray ◽  
Multiplex Polymerase Chain Reaction ◽  
Blood Group Antigens ◽  
Microarray Hybridization ◽  
Chain Reaction ◽  
Polymerase Chain

Potential Benefits of Extended Genotype Matching to Chronically Transfused Patients with Sickle Cell Disease

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 2144-2144 ◽  
Author(s):  
Ricardo Helman ◽  
Rodolfo Cancado ◽  
Mariza A Mota ◽  
Marcia R Dezan ◽  
Jose Mauro Kutner ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Blood Group ◽  
Sickle Cell ◽  
Conflicts Of Interest ◽  
Blood Group Antigens ◽  
Cell Disease ◽  
True Blood ◽  
Erythrocyte Antigen ◽  
Antibody Specificities

Abstract Abstract 2144 Background: Management of RBC alloimmunization in Sickle Cell Disease (SCD) patients has been the subject of much debate, and currently there is no standard approach. Many programs transfuse SCD patients with RBCs that are phenotype-matched for D, C, c, E, e and K. Although these approaches reduce the incidence of alloantibody production, patients still become alloimmunized. Based on this we aimed to identify the rates of alloimmunization in chronically transfused SCD patients and compare the phenotyping with genotyping methods to find a better way to match RBC units to those patients. Methods: We selected 45 SCD patients (homozygous for hemoglobin S) with multiple transfusions, previously phenotyped for ABO, Rh (D, C, c, E, e) and K1. Phenotypes were determined by hemagglutination using gel cards (Diamed® ). Genotypes were determined by a DNA array using the Human Erythrocyte Antigen BeadChip (“HEA”) from Bioarray Solutions. All SCD patients included in this study were in chronical transfusion program; receiving multiple transfusions. The median age was 24y; there were 28(62%) females and 17(37.8%) males. The median of transfusions were 53 (5–78) and 40 (88.9%) patients received more than 20 phenotype-matched units for Rh (D, C, c, E, e) and K1. Results: Of the 45 SCD patients selected, 11 (24.4%) had alloantibodies. The antibody specificities found in these patients were anti-D, -C, -CW, -E, -Jka, -Jkb, -Fya, -Dia, -s. Although the patients were receiving Rh and K phenotype-matched units 8 (17%) of them became alloimmunized to Rh antigens and on those patients we found discrepancies between the previous phenotype and genotype-derived phenotype. Our results showed that the risk of immunization increases in patients over 40 years old (p= 0.05) and with the number of transfusion events. Patients with more than 20 RBC transfusions have a tendency for alloimmunization (p=0.65). We also observed that genotyping was more effective than hemagglutination in determining patient's correct phenotype. Conclusion: Our data show that even with the implementation of Rh and K phenotype-matching in chronically transfused patients with SCD, they still become alloimmunized to other antigens with high immunization risk and also to Rh antigens due to the limitations of the hemagglutination. The relevance of genotype determination of blood groups for the management of multiple transfused patients with SCD has been demonstrated by allowing the determination of the true blood group genotype, by assisting in the identification of suspected alloantibodies and in the selection of antigen-negative. As donor genotyping for the most clinically relevant blood group antigens by automated DNA techniques are becoming available, extended genotype matching should be considered in this group of patients. Disclosures: No relevant conflicts of interest to declare.

Sign in / Sign up

Export Citation Format

Share Document