scholarly journals EFFECT OF TRANSGLUTAMINASE TREATMENTON TRANSITION OF SOLIDS FROM MILK TO CHEESE

Author(s):  
A. I. Yashkin ◽  
V. B. Mazalevskij

Transglutaminase is an enzyme that forms cross-links between protein molecules and affects such protein properties as the ability to gel, thermal stability, water retention, etc. One of the important tasks of the food industry is to increase the yield of products, in particular soft cheeses, in the production of which a significant part of the whey with dissolved substances is separated from milk. Therefore, the aim of the work was to study the effect of transglutaminase on the transition of milk solids to cheese, depending on the stage of application of the enzyme. Transglutaminase in an amount of 0.05% by weight of milk (1.6 units in terms of 1 g of protein) was introduced in two versions: simultaneously with the milk-clotting enzyme and after cutting the clot. It was found that the use of transglutaminase does not affect the duration of acid-rennet coagulation of milk. The data obtained indicate that when transglutaminase is introduced into milk simultaneously with the milk-converting enzyme, the transition of milk solids to cheese increases by 5.15%, including fat by 3.07%, compared to samples without transglutaminase. When transglutaminase is added at the stage of processing the clot, a denser consistency of cheese is formed.

Author(s):  
D.V. Abramov ◽  
◽  
D.S. Myagkonosov ◽  
I.N. Delitskaya ◽  
V.A. Mordvinova ◽  
...  

2021 ◽  
Vol 69 (9) ◽  
pp. 2784-2792
Author(s):  
Fanqiang Meng ◽  
Haizhen Zhao ◽  
Fengxia Lu ◽  
Xiaomei Bie ◽  
Zhaoxin Lu ◽  
...  

1971 ◽  
Vol 35 (9) ◽  
pp. 1398-1401
Author(s):  
Juhyun Yu ◽  
Gakuzo Tamura ◽  
Kei Arima

1971 ◽  
Vol 35 (8) ◽  
pp. 1194-1199
Author(s):  
Juhyun Yu ◽  
Gakuzo Tamura ◽  
Kei Arima

2014 ◽  
Vol 10 (2) ◽  
pp. 211-222 ◽  
Author(s):  
Valentin Désiré Guiama ◽  
Robert Germain Beka ◽  
Esther Ngah ◽  
David Gabriel Libouga ◽  
Dominique Vercaigne-Marko ◽  
...  

Abstract This study investigated a novel procedure of Solanum aethiopicum Shum fruits extract (SASFE) preparation using multivariate experimental designs as factorial and Box–Behnken. The thermal stability of optimized extract as well as its influence on the milk solids in curd was determined. The results showed that extraction time, fruit maturity and pH did not affect significantly SASFE preparation, while the amount of fruits, extraction temperature and NaCl concentration of extractant had a significant effect (p < 0.05). The greatest coagulant index was obtained under the following conditions: 12.5% of fruits, 25°C of extraction temperature and 4% NaCl concentration of extractant. It was thermosensitive and exhibited optimum temperature at 50°C. There was no statistical difference between SASFE and calf rennet in terms of solids yield in curd, estimated yield and actual yield. On the basis of these results, SASFE can be used as a vegetable alternative to calf rennet.


Author(s):  
Yoko Takyu ◽  
Taro Asamura ◽  
Ayako Okamoto ◽  
Hiroshi Maeda ◽  
Michio Takeuchi ◽  
...  

Abstract Aspergillus oryzae RIB40 has 11 aspartic endopeptidase genes. We searched for milk-clotting enzymes based on the homology of the deduced amino acid sequence with chymosins. As a result, we identified a milk-clotting enzyme in A. oryzae. We expected other Aspergillus species to have a homologous enzyme with milk-clotting activity, and we found the most homologous aspartic endopeptidase from A. luchuensis had milk-clotting activity. Surprisingly, two enzymes were considered as vacuole enzymes according to a study on A. niger proteases. The two enzymes from A. oryzae and A. luchuensis cleaved a peptide between the 105Phe-106Met bond in κ-casein, similar to chymosin. Although both enzymes showed proteolytic activity using casein as a substrate, the optimum pH values for milk-clotting and proteolytic activities were different. Furthermore, the substrate specificities were highly restricted. Therefore, we expected that the Japanese traditional fermentation agent, koji, could be used as an enzyme source for cheese production.


2014 ◽  
Vol 61 (9) ◽  
pp. 444-447 ◽  
Author(s):  
Kazuo Nakamura ◽  
Nahoko Kobayashi ◽  
Morimasa Tanimoto

1985 ◽  
Vol 49 (6) ◽  
pp. 1621-1631 ◽  
Author(s):  
Hideyuki KOBAYASHI ◽  
Isao KUSAKABE ◽  
Saeko YOKOYAMA ◽  
Kazuo MURAKAMI

Sign in / Sign up

Export Citation Format

Share Document