Attempted Type Specific Diagnosis of Dengue Virus Infection by the Indirect Fluorescent Antibody Method Directed at Differentiating IgM and IgG Responses

1973 ◽  
Vol 142 (2) ◽  
pp. 697-702 ◽  
Author(s):  
K. Vathanophas ◽  
W. M. Hammon ◽  
R. W. Atchison ◽  
G. E. Sather
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Fluorescent Antibody ◽  
Indirect Fluorescent Antibody ◽  
Dengue Virus Infection ◽  
Specific Diagnosis ◽  
Antibody Method ◽  
Fluorescent Antibody Method

Localization of pancreatic enzymes in ultrathin frozen sections stained with metal labeled antibody

1978 ◽  
Vol 36 (2) ◽  
pp. 90-91
Author(s):  
Kenjiro Yasuda
Keyword(s):  
Fluorescent Antibody ◽  
Pancreatic Enzymes ◽  
Tissue Preparation ◽  
Zymogen Granules ◽  
Frozen Sections ◽  
En Bloc ◽  
Antibody Method ◽  
Ultrathin Frozen Sections ◽  
Fluorescent Antibody Method

Localization of amylase,chymotrypsinogen and trypsinogen in pancreas was demonstrated by Yasuda and Coons (1966), by using fluorescent antibody method. These enzymes were naturally found in the zymogen granules. Among them, amylase showed a diffuse localization around the nucleus, in addition to the zymogen granules. Using ferritin antibody method, scattered ferritin granules were also found around the Golgi area (Yasuda et al.,1967). The recent advance in the tissue preparation enables the antigen to be localized in the ultrathin frozen sections, by applying the labeled antibodies onto the sections instead of staining the tissue en bloc.The present study deals with the comparison of the localization of amylase and lipase demonstrated by applying the bismuth-labeled, peroxidase-labeled and ferritin-labeled antibody methods on the ultrathin frozen sections of pancreas, and on the blocks of the same tissue.

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