Iron overload causes osteoporosis in thalassemia major patients through interaction with transient receptor potential vanilloid type 1 (TRPV1) channels

AbstractTransient receptor potential vanilloid type 1 (TRPV1) channels are activated by heat, vanilloids, and extracellular protons. Cryo-EM has revealed various conformations of TRPV1, and these structures suggest an intramolecular twisting motion in response to ligand binding. However, limited experimental data support this observation. Here we analyzed the intramolecular motion of TRPV1 using diffracted X-ray tracking (DXT). DXT analyzes trajectories of Laue spots generated from attached gold nanocrystals, and provides picometer spatial and microsecond time scale information about intramolecular motion. We observed that both an agonist and a competitive antagonist evoked rotating bias in TRPV1, though these biases were in opposing directions. Furthermore, the rotational bias generated by capsaicin was reversed between the wild type and the capsaicin-insensitive Y511A mutant. Our findings bolster the understanding of the mechanisms used activation and modulation of TRP channels, and this knowledge can be exploited for pharmacological usage such as inhibitor design.

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Anticonvulsant Effects ofN-Arachidonoyl-Serotonin, a Dual Fatty Acid Amide Hydrolase Enzyme and Transient Receptor Potential Vanilloid Type-1 (TRPV1) Channel Blocker, on Experimental Seizures: The Roles of Cannabinoid CB1 Receptors and TRPV1 Channels

Basic & Clinical Pharmacology & Toxicology ◽

10.1111/bcpt.12232 ◽

2014 ◽

Vol 115 (4) ◽

pp. 330-334 ◽

Cited By ~ 13

Author(s):

Luciano R. Vilela ◽

Daniel C. Medeiros ◽

Antonio Carlos P. de Oliveira ◽

Marcio F. Moraes ◽

Fabricio A. Moreira

Keyword(s):

Transient Receptor Potential ◽

Fatty Acid Amide Hydrolase ◽

Acid Amide ◽

Receptor Potential ◽

Transient Receptor Potential Vanilloid ◽

Trpv1 Channel ◽

Trpv1 Channels ◽

Amide Hydrolase ◽

Transient Receptor

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Interactions between Chemesthesis and Taste: Role of TRPA1 and TRPV1

International Journal of Molecular Sciences ◽

10.3390/ijms22073360 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3360

Author(s):

Mee-Ra Rhyu ◽

Yiseul Kim ◽

Vijay Lyall

Keyword(s):

Transient Receptor Potential ◽

Taste Receptor ◽

Sensory Nerve ◽

Receptor Potential ◽

Transient Receptor Potential Vanilloid ◽

Trpv1 Channels ◽

Trigeminal Neurons ◽

Calcitonin Gene ◽

Transient Receptor

In addition to the sense of taste and olfaction, chemesthesis, the sensation of irritation, pungency, cooling, warmth, or burning elicited by spices and herbs, plays a central role in food consumption. Many plant-derived molecules demonstrate their chemesthetic properties via the opening of transient receptor potential ankyrin 1 (TRPA1) and transient receptor potential vanilloid 1 (TRPV1) channels. TRPA1 and TRPV1 are structurally related thermosensitive cation channels and are often co-expressed in sensory nerve endings. TRPA1 and TRPV1 can also indirectly influence some, but not all, primary taste qualities via the release of substance P and calcitonin gene-related peptide (CGRP) from trigeminal neurons and their subsequent effects on CGRP receptor expressed in Type III taste receptor cells. Here, we will review the effect of some chemesthetic agonists of TRPA1 and TRPV1 and their influence on bitter, sour, and salt taste qualities.

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