scholarly journals Evaluation of the antibody response against Hepatitis B Virus infection in patients on maintenance hemodialysis: A Pilot Study

1970 ◽  
pp. 15-22
Author(s):  
S Shahin ◽  
Md Khoybar Ali ◽  
A Farhana ◽  
K Matira
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Antibody Response ◽  
Medical Science ◽  
Clinical History ◽  
Maintenance Hemodialysis ◽  
Natural Immunity ◽  
Hepatitis B Virus Infection ◽  
B Virus

Objective: This study was undertaken to evaluate the antibody response of hepatitis B virus infection in patients on maintenance hemodialysis (MHD) by detecting different viral markers. Method: Study subjects comprised a total of 88 chronic kidney disease (CKD) patients from Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM) and Bangabandhu Sheikh Mujib Medical University (BSMMU). Of them 63 patients on MHD and 25 predialysis patients served as cases and controls respectively. Clinical history was taken and serological markers for HBV (HBsAg, Anti-HBs, and Anti-HBc) were determined by using ELISA. Results: Hepatitis B virus was positive in 1.6% of maintenance hemodialysis (MHD) patients and in 16% of controls (p<0.02). Anti-HBc antibody was positive in 62% of dialysis patients and 72% of controls (p=NS) and the positivity was significantly associated in dialysis subjects with longer duration of dialysis (18 ± 22 vs. 10 ± 7, months, p<0.04), multiple units of blood transfusions (22 ± 29 vs. 10 ± 12, units, p<0.04) and more reuse of dialyzer (3 ± 1 vs. 2 ± 1, times, p<0.03) than the negative ones. Among MHD patients 84% were vaccinated against HBV with a schedule of 3 (79%) and 4 (21%) doses and protective antibody titer (>10 IU/L) was found in 57%. None of the controls were vaccinated but 66% had protective titer indicating post exposure natural immunity. Conclusions: Hepatitis B virus positivity was significantly higher among the predialysis subjects compared to dialysis group. Key words: Hepatitis B virus, Antibody response, Hemodialysis doi: 10.3329/bjms.v8i1.3185 Bangladesh Journal of Medical Science Vol.8 No. 1-2; 2009 15-21

Poor IgM antibody response to hepatitis B core antigen in infants with hepatitis B virus infection

1989 ◽  
Vol 115 (4) ◽  
pp. 609-611 ◽  
Author(s):  
Harold S. Margolis ◽  
Zhi-yi Xu ◽  
Omana V. Nainan ◽  
Pei-Ying Ou-Yang ◽  
Shu-Cheng Duan ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Antibody Response ◽  
Core Antigen ◽  
Hepatitis B Virus Infection ◽  
Igm Antibody ◽  
B Virus ◽  
Hepatitis B Core Antigen

scholarly journals Occult hepatitis B virus infection in maintenance hemodialysis patients: prevalence and mutations in “a” determinant

2019 ◽  
Author(s):  
Yun Tang ◽  
Xiangqin Liu ◽  
Daqing Hong ◽  
Xiangheng Lu ◽  
Qiang He ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Real Time Pcr ◽  
Nested Pcr ◽  
Hbv Dna ◽  
The Other ◽  
Maintenance Hemodialysis ◽  
Hepatitis B Virus Infection ◽  
B Virus

Abstract Background：Occult hepatitis B virus infection (OBI) is rare and its diagnosis is often overlooked, but there is still a risk of HBV transmission. To analysis the prevalence of OBI among maintenance hemodialysis (MHD) patients in Sichuan Provincial People’s Hospital, the molecular biological characteristics of OBI and the mutation of “a” determinant were investigated. Method: A total of 330 patients undergoing hemodialysis at Sichuan Provincial People’s Hospital and its satellite dialysis units were tested for HBV markers by ELISA. HBV-DNA was detected by real-time PCR in patients with HBsAg negative/ HBcAb positive to investigate the prevalence of OBI. For plasma samples with persistent HBV DNA positive, the S gene was determined by nested PCR, and the sequence of gene mutation was compared with the standard sequence. Result: Among 330 patients, ages range from 27 to 95 with a mean of 60.66 years old and majority of patients (57.6%) > 60 years old. Serum from 165 of 180 HBsAg negative/HBcAb positive individuals were tested for HBV DNA by real-time PCR. 7 of 165 patients had low level of HBV DNA. Of those, 5 individuals were both HBcAb and HBsAb positive, and 2 individuals were HBcAb positive alone. After 2 years follow-up, 2 individuals with HBcAb positive alone were both tested HBV DNA again, but the other patients were not. Serum from the 2 patients were used nested PCR to confirm the genotype and the mutation of S gene. We found they both were genotype B. Amino acid sequencing confirmed that one had no mutations and the other one had Q128R, T131N, M133S, F134L and D144E mutants in “a” determinant. Conclusion: Chronic HBV infection was 7.3% (24/330) and OBI was 2.1% (7/330) in our hospital hemodialysis center. The genotype of patients with OBI were genotype B. Mutations of Q128R，T131N，M133S，F134L and D144E might be the potential sites associated with OBI.

Virus-specific coexpression of T-bet and Eomes defines highly functional CD8 and CD4 T cells in hepatitis B virus infection

2017 ◽  
Vol 55 (05) ◽  
pp. e1-e27
Author(s):  
H Karimzadeh ◽  
P Kurktschiev ◽  
D Scharafin ◽  
W Schraut ◽  
NH Grüner ◽  
...  
Keyword(s):  
T Cells ◽  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Cd4 T Cells ◽  
Hepatitis B Virus Infection ◽  
B Virus
Sign in / Sign up

Export Citation Format

Share Document