scholarly journals In vitro Plantlet Regeneration from Callus Culture of Trachyspermum copticum

2017 ◽  
Vol 27 (1) ◽  
pp. 13-20
Author(s):  
Hamze Teymourian ◽  
Mohammad Ali Ebrahimi ◽  
Masoud Tohidfar ◽  
Nazi Farsaloon ◽  
Nasim Zarinpanjeh
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Cotyledonary Node ◽  
Plantlet Regeneration ◽  
Shoot Induction ◽  
Best Response ◽  
Trachyspermum Copticum

The effect of explant sources and plant growth regulators on callus induction and plantlet regeneration of Trachyspermum copticum were explored. Different explants including hypocotyl, cotyledonary node and leaf were cultured on MS supplemented with different combinations and concentrations of plant growth regulators including 2,4‐D (0.2‐3 0.5 mg/l), NAA (2 mg/l), BAP (1‐3 mg/l), Kn (0.5 mg/l) and IAA (0.8 mg/l). The best response for callus induction (100%) as well as quality was observed from cotyldonary node segments cultured on MS supplemented with 2, 4‐D at 1 mg/l in combination with Kn at 0.5 mg/l. Calli derived from various explants were subcultured on shoot induction media with different compositions and concentrations of medium. MS without any plant growth regulator promoted the highest frequency of shoot regeneration (100%) and also mean number of developed shoots per explants (3.8) showed the same result. Regenerated shoots were then rooted on three‐fourth strength MS with 75% efficiency after 30 days.Plant Tissue Cult. & Biotech. 27(1): 13-20, 2017 (June)

scholarly journals Effect of Different Plant Growth Regulators on In-vitro Callus Induction in Carica papaya (cv. Pusa Nanha)

2019 ◽  
Vol 8 (07) ◽  
pp. 1217-1225
Author(s):  
Nisha Malik ◽  
Rakesh Singh Sengar ◽  
Manoj Kumar Yadav ◽  
Shiv Kumar Singh ◽  
Gopal Singh ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Carica Papaya

Production of Isoflavonoids in Callus Cultures of Pueraria candollei var. mirifica

2009 ◽  
Vol 64 (3-4) ◽  
pp. 239-243 ◽  
Author(s):  
Latiporn Udomsuk ◽  
Kanokwan Jarukamjorn ◽  
Hiroyuki Tanaka ◽  
Waraporn Putalun
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Culture Medium ◽  
Callus Cultures ◽  
Stem Explants ◽  
Ms Medium ◽  
Shoot Induction ◽  
Pueraria Candollei

Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66 - 100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.

scholarly journals Effects of different plant growth regulators on in vitro callus induction in physic nut (Jatropha curcus L.)

2015 ◽  
Vol 7 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Sunil Kumar ◽  
Virendra Kumar ◽  
Manoj Kumar Sharma ◽  
Narendra Kumar ◽  
Anil Kumar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Culture Media ◽  
Nodal Segments ◽  
Leaf Lamina ◽  
Physic Nut ◽  
Cotyledonary Nodes

Jatropha (Jatropha curcas L.) is an oil bearing crop growing in tropical and subtropical parts of the world. The present study was undertaken to investigate the effects of different plant growth regulators on in vitro callus induction in physic nut (J. curcus). In the present study, it was observed that all the explants viz., leaf lamina, petioles, nodal segments and cotyledonary nodes showed good callus induction responses on various culture media thus tried. Leaf lamina and petioles showed 100.0% callus induction responses on different MS media supplemented with auxins and cytokinins alone or in combinations whereas, nodal segments and cotyledonary nodes showed maximum 89.6% and 83.9% callus induction respectively. The presence of 2, 4-D in culture media with auxins or cytokinins was essential for good callus growth. Among different explants tried, leaf lamina was the best responding explants and MS-13 media supplemented with 5×10-6 M NAA and 10-5 M 2, 4-D is the best callusing and growth supporting medium. However, the regenerative competence of the callus tissues can differ depending on the type of explants used because certain types of plant tissues have more favorable regeneration responses than others. Callus induction rate from all explant types was highest than other reports. The results obtained in the present study would facilitate the high callus induction and regeneration responses in J. curcus for its improvement using biotechnological tools.

scholarly journals Effect of the Carbon Source and Plant Growth Regulators (PGRs) in the Induction and Maintenance of an In Vitro Callus Culture of Taraxacum officinale (L) Weber Ex F.H. Wigg

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1181
Author(s):  
María Eugenia Martinez ◽  
Lorena Jorquera ◽  
Paola Poirrier ◽  
Katy Díaz ◽  
Rolando Chamy
Keyword(s):  
Carbon Source ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Callus Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Large Scale ◽  
Taraxacum Officinale ◽  
Naphthaleneacetic Acid

Taraxacum officinale (L.) Weber ex F.H. Wigg, commonly known as dandelion, is a cosmopolitan and perennial weed, which has medicinal properties. In vitro propagation methods are widely used on plants that have difficulties in cultivation and, consequently, low extraction yields of active metabolites. Thus, callus culture has been considered to be useful for the accumulation of several metabolites. In this study, we aimed to establish an efficient protocol for callus induction and maintenance of T. officinale, for which explant type, carbon source, light conditions, and nine different combinations of plant growth regulators (PGRs), such as 1-naphthaleneacetic acid (NAA) (from 0.05 to 0.5 mg/L) and 6-benzylaminopurine acid (BAP) (from 0.5 to 3.0 mg/L), were evaluated. The results showed that hypocotyls and roots from sterile seedlings are the best sources for callus induction, with 100% of callogenesis at every condition tested, and more than 95% of viability and friability. Complete darkness and a medium supplemented with sucrose at 2.3% (w/v) and 0.5 mg/L of NAA and 0.5 mg/L of BAP were the best conditions for callus induction, showing callus with low organogenesis and high friability. This study provides a basis for future studies on improving large-scale callus propagation and further establishment of suspension culture systems for commercial purposes.

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