Amniotic Membrane Transplanted in Conjunctiva as a Mesenchymal Stem Cells Carrier for Limbal Stem Cell Deficiency

2011 ◽  
Vol 52 (9) ◽  
pp. 1104
Author(s):  
Tae Hyung Kim ◽  
Jae Hoon Jeong ◽  
Yeoun Sook Chun ◽  
Jae Chan Kim
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Amniotic Membrane ◽  
Limbal Stem Cell Deficiency ◽  
Limbal Stem Cell

Reconstruction of limbal stem cell deficient corneal surface with induced human bone marrow mesenchymal stem cells on amniotic membrane

2014 ◽  
Vol 163 (3) ◽  
pp. 200-210 ◽  
Author(s):  
Che Man Rohaina ◽  
Kong Yong Then ◽  
Angela Min Hwei Ng ◽  
Wan Haslina Wan Abdul Halim ◽  
Aida Zairani Mohd Zahidin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Amniotic Membrane ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Corneal Surface ◽  
Marrow Mesenchymal Stem Cells ◽  
Limbal Stem Cell

Corneal surface reconstruction using adult mesenchymal stem cells in experimental limbal stem cell deficiency in rabbits

2009 ◽  
Vol 89 (8) ◽  
pp. 741-748 ◽  
Author(s):  
Helga Reinshagen ◽  
Claudia Auw-Haedrich ◽  
Ruediger V. Sorg ◽  
Daniel Boehringer ◽  
Philipp Eberwein ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Surface Reconstruction ◽  
Limbal Stem Cell Deficiency ◽  
Corneal Surface ◽  
Adult Mesenchymal Stem Cells ◽  
Limbal Stem Cell ◽  
Corneal Surface Reconstruction

scholarly journals Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative Ophthalmology

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1134
Author(s):  
Julia I. Khorolskaya ◽  
Daria A. Perepletchikova ◽  
Daniel V. Kachkin ◽  
Kirill E. Zhurenkov ◽  
Elga I. Alexander-Sinkler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Fluorescent Protein ◽  
Rabbit Model ◽  
Stem Cell Markers ◽  
Epithelial Stem Cells ◽  
Limbal Stem Cell ◽  
Green Fluorescent

The development of cell-based approaches to the treatment of various cornea pathologies, including limbal stem cell deficiency (LSCD), is an area of current interest in regenerative biomedicine. In this context, the shortage of donor material is urgent, and limbal mesenchymal stem cells (L-MSCs) may become a promising cell source for the development of these novel approaches, being established mainly within the rabbit model. In this study, we obtained and characterized rabbit L-MSCs and modified them with lentiviral transduction to express the green fluorescent protein EGFP (L-MSCs-EGFP). L-MSCs and L-MSCs-EGFP express not only stem cell markers specific for mesenchymal stem cells but also ABCG2, ABCB5, ALDH3A1, PAX6, and p63a specific for limbal epithelial stem cells (LESCs), as well as various cytokeratins (3/12, 15, 19). L-MSCs-EGFP have been proven to differentiate into adipogenic, osteogenic, and chondrogenic directions, as well as to transdifferentiate into epithelial cells. The possibility of using L-MSCs-EGFP to study the biocompatibility of various scaffolds developed to treat corneal pathologies was demonstrated. L-MSCs-EGFP may become a useful tool for studying regenerative processes occurring during the treatment of various corneal pathologies, including LSCD, with the use of cell-based technologies.

Amniotic membrane transplantation with conjunctival limbal autograft for total limbal stem cell deficiency

Ophthalmology ◽  
2003 ◽  
Vol 110 (8) ◽  
pp. 1585-1592 ◽  
Author(s):  
Mario A Meallet ◽  
Edgar M Espana ◽  
Martin Grueterich ◽  
Seng-Ei Ti ◽  
Eiki Goto ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Membrane ◽  
Limbal Stem Cell Deficiency ◽  
Amniotic Membrane Transplantation ◽  
Limbal Stem Cell

scholarly journals Sutureless Amniotic Membrane Transplantation for Partial Limbal Stem Cell Deficiency

2008 ◽  
Vol 145 (5) ◽  
pp. 787-794 ◽  
Author(s):  
Ahmad Kheirkhah ◽  
Victoria Casas ◽  
Vadrevu K. Raju ◽  
Scheffer C.G. Tseng
Keyword(s):  
Stem Cell ◽  
Amniotic Membrane ◽  
Limbal Stem Cell Deficiency ◽  
Amniotic Membrane Transplantation ◽  
Limbal Stem Cell

Allogenic Limbo-keratoplasty with Conjunctivoplasty, Mitomycin C, and Amniotic Membrane for Bilateral Limbal Stem Cell Deficiency

Ophthalmology ◽  
2012 ◽  
Vol 119 (5) ◽  
pp. 930-937 ◽  
Author(s):  
Philipp Eberwein ◽  
Daniel Böhringer ◽  
Johannes Schwartzkopff ◽  
Florian Birnbaum ◽  
Thomas Reinhard
Keyword(s):  
Stem Cell ◽  
Mitomycin C ◽  
Amniotic Membrane ◽  
Limbal Stem Cell Deficiency ◽  
Limbal Stem Cell
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