scholarly journals Detection of Spinal Muscular Atrophy Using a Duplexed Real-Time PCR Approach With Locked Nucleic Acid-Modified Primers

2021 ◽  
Vol 41 (1) ◽  
pp. 101-107
Author(s):  
Jianyan Pan ◽  
Chunhua Zhang ◽  
Yanling Teng ◽  
Sijing Zeng ◽  
Siyi Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Spinal Muscular Atrophy ◽  
Real Time ◽  
Real Time Pcr ◽  
Muscular Atrophy ◽  
Locked Nucleic Acid

scholarly journals Locked Nucleic Acid Probe-Based Real-Time PCR Assay for the Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143444 ◽  
Author(s):  
Yong Zhao ◽  
Guilian Li ◽  
Chongyun Sun ◽  
Chao Li ◽  
Xiaochen Wang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Locked Nucleic Acid ◽  
Pcr Assay ◽  
Locked Nucleic Acid Probe

scholarly journals Locked nucleic acid inhibits amplification of contaminating DNA in real-time PCR

BioTechniques ◽  
2005 ◽  
Vol 38 (4) ◽  
pp. 605-610 ◽  
Author(s):  
Lone Hummelshoj ◽  
Lars P. Ryder ◽  
Hans O. Madsen ◽  
Lars K. Poulsen
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Locked Nucleic Acid

scholarly journals Novel locked nucleic acid (LNA)-based probe for the rapid identification of Chlamydia suisusing real-time PCR

2014 ◽  
Vol 10 (1) ◽  
Author(s):  
Paweł Lis ◽  
Aleksandra Kumala ◽  
Mirosław Spalinski ◽  
Krzysztof Rypula
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Identification ◽  
Locked Nucleic Acid

scholarly journals Use of locked nucleic acid oligonucleotides as hybridization/FRET probes for quantification of 16S rDNA by real-time PCR

BioTechniques ◽  
2005 ◽  
Vol 38 (1) ◽  
pp. 29-31 ◽  
Author(s):  
Oliver Goldenberg ◽  
Olfert Landt ◽  
Ralf R. Schumann ◽  
Ulf B. Göbel ◽  
Lutz Hamann
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
16S Rdna ◽  
Real Time Pcr ◽  
Locked Nucleic Acid

Fast and sensitive real-time PCR detection of major antiviral drug-resistance mutations in chronic hepatitis B patients using a predesigned panel of Locked-Nucleic-Acid TaqMan probes

2021 ◽  
Author(s):  
Son V. Chu ◽  
Son T. Vu ◽  
Hang M. Nguyen ◽  
Ngan T. Le ◽  
Phuong T. Truong ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Chronic Hepatitis ◽  
Nucleic Acid ◽  
Hepatitis B ◽  
Real Time ◽  
Chronic Hepatitis B ◽  
Real Time Pcr ◽  
Locked Nucleic Acid ◽  
Resistance Mutations ◽  
Drug Resistance Mutations

We developed a novel real-time PCR assay that simultaneously evaluates eleven major nucleos(t)ide antiviral (NA) drug-resistance mutations (mt) in chronic hepatitis B patients (CHB), including L180M, M204I/V, and V207M (lamivudine (LMV) resistance), N/H238A/T (Adefovir (ADF) resistance) which are circulating in Vietnam; T184G/L, S202I, M250V (entecavir (ETV) resistance), and A194T (tenofovir resistance) which have been recently reported in several studies across the globe. We detected drug-resistant mt in HBV samples using our predesigned panel of allele-specific locked-nucleic acid (LNA) probes. Our assay had a high sensitivity of 5% in a low HBV DNA population of ≥5 ×10 3 IU/mL and was validated in a cohort of 130 treatment-naive children and 98 NA-experienced adults with CHB. Single-point mt for LMV- and ADF-resistance were detected in 57.7% and 54.1% of the child and adult samples, respectively, with rtV207M (42.3%, children; 36.7%, adults) and rtN238T/A (15.4%, children; 16.3, adults) being the most frequent mt in these populations. Multiple-point mt including rtL180M-rtM204V- rtN238A and rtL180M-rtM204I were only identified in two children, resulting in LMV-ADF-resistance and reduced ETV susceptibility. In conclusion, this assay accurately identified the mt profile of children (98.4%) and adults (91.2%) with CHB, which is comparable to established methods. This fast and sensitive screening method can be used for the detection of major NA-resistant mt circulating in developing countries, as well as providing a model for the development of similar mt-detection assays, especially for use in non-hospitalised patients who need their results within half a day before starting treatment.

Multiplex TaqMan locked nucleic acid real-time PCR for the differential identification of various meat and meat products

Meat Science ◽  
2018 ◽  
Vol 137 ◽  
pp. 41-46 ◽  
Author(s):  
Rusu Xu ◽  
Shuang Wei ◽  
Guangbiao Zhou ◽  
Jiao Ren ◽  
Zhongyong Liu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Meat Products ◽  
Locked Nucleic Acid ◽  
Differential Identification

scholarly journals Library of Prefabricated Locked Nucleic Acid Hydrolysis Probes Facilitates Rapid Development of Reverse-Transcription Quantitative Real-Time PCR Assays for Detection of Novel Influenza A/H1N1/09 Virus

2009 ◽  
Vol 55 (12) ◽  
pp. 2218-2222 ◽  
Author(s):  
Jürgen J Wenzel ◽  
Heiko Walch ◽  
Markus Bollwein ◽  
Hans Helmut Niller ◽  
Waltraud Ankenbauer ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Influenza A ◽  
Rapid Development ◽  
Locked Nucleic Acid ◽  
Quantitative Real Time Pcr ◽  
Influenza A H1n1 ◽  
Novel Influenza A

Abstract Background: The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. Methods: We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLibrary (UPL)—a collection of 165 presynthesized, fluorescence-labeled locked nucleic acid (LNA) hydrolysis probes—specifically to detect the novel influenza A virus. We evaluated candidate primer/UPL-probe pairs with 28 novel influenza A/H1N1/09 patient samples of European and Mexican origin. Results: Of 14 assays in the hemagglutinin (HA) and neuraminidase (NA) genes, 12 detected viral nucleic acids from diluted patient samples without need for further optimization. We characterized the diagnostic specificity of the 2 best-performing assays with a set of samples comprising various influenza virus strains of human and animal origin that showed no cross-reactivity. The diagnostic sensitivity of these 2 primer/probe combinations was in the range of 100–1000 genomic copies/mL. In comparison to a reference assay recommended by the German health authorities, the analytical sensitivities and specificities of the assays were equivalent. Conclusions: Facing the emergence of novel influenza A/H1N1/09, we were able to develop, within 2 days, a set of sensitive and specific RT-qPCR assays for the laboratory diagnosis of suspected cases. H1N1/09 served as a model to show the feasibility of the UPL approach for the expedited development of new diagnostic assays to detect emerging pathogens.

Detection and measurement of benzimidazole resistance alleles in Haemonchus contortus using real-time PCR with locked nucleic acid Taqman probes

2007 ◽  
Vol 144 (3-4) ◽  
pp. 304-312 ◽  
Author(s):  
Thomas K. Walsh ◽  
Alison A. Donnan ◽  
Frank Jackson ◽  
Philip Skuce ◽  
Adrian J. Wolstenholme
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Haemonchus Contortus ◽  
Locked Nucleic Acid ◽  
Taqman Probes ◽  
Benzimidazole Resistance
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