scholarly journals Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUTs Strain

2019 ◽  
Vol 7 ◽  
Author(s):  
David Rodrigues ◽  
Omar Pillaca-Pullo ◽  
Karin Torres-Obreque ◽  
Juan Flores-Santos ◽  
Ignacio Sánchez-Moguel ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pichia Pastoris ◽  
Fed Batch ◽  
Batch Production ◽  
Recombinant Pichia Pastoris

Asparaginase production by a recombinant Pichia pastoris strain harbouring Saccharomyces cerevisiae ASP3 gene

2006 ◽  
Vol 39 (7) ◽  
pp. 1457-1463 ◽  
Author(s):  
Maria Antonieta Ferrara ◽  
Neuza M.B. Severino ◽  
José João Mansure ◽  
Adriana Sotero Martins ◽  
Edna M.M. Oliveira ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pichia Pastoris ◽  
Pastoris Strain ◽  
Recombinant Pichia Pastoris

scholarly journals Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Christoph Gmeiner ◽  
Amirhossein Saadati ◽  
Daniel Maresch ◽  
Stanimira Krasteva ◽  
Manuela Frank ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Process ◽  
Fed Batch ◽  
Plant Peroxidase ◽  
Recombinant Pichia Pastoris

scholarly journals Influence of trace elements supplementation on the production of recombinant frutalin by Pichia pastoris KM71H in fed-batch process

2013 ◽  
Vol 67 (7) ◽  
Author(s):  
Marcela Wanderley ◽  
Carla Oliveira ◽  
Danyelly Bruneska ◽  
Lucília Domingues ◽  
José Lima Filho ◽  
...  
Keyword(s):  
Trace Elements ◽  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Production ◽  
Batch Process ◽  
Fermentation Medium ◽  
Fed Batch ◽  
Stirred Tank Bioreactor ◽  
Recombinant Pichia Pastoris ◽  
Fed Batch Fermentation

AbstractFrutalin, a galactose-specific lectin used to detect specific tumour markers, is a protein with low expression level in breadfruit. In the present study, fed-batch fermentation in a stirred tank bioreactor was used as a strategy to enhance protein production by a recombinant Pichia pastoris KM71H. By using this process, the production of recombinant frutalin was 4-fold higher than the value obtained in shaker flasks batch assays. Supplementation of the fermentation medium with trace elements (Pichia trace minerals, PTM) was also evaluated in order to stimulate production of the recombinant protein. The addition of PTM to the minimum medium afforded a recombinant protein production of 13.4 mg L−1, which was 2.5-fold higher than that achieved from the culture medium without PTM supplementation. These results are significant as the development of strategies to improve the production of recombinant frutalin may broaden its application in cancer diagnosis.

scholarly journals HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation

2014 ◽  
Vol 45 (2) ◽  
pp. 485-490 ◽  
Author(s):  
Andrea B. Zepeda ◽  
Carolina A. Figueroa ◽  
Dulcineia S.P. Abdalla ◽  
Andrea Q. Maranhão ◽  
Patricio H. Ulloa ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Hsp90 Expression ◽  
Recombinant Pichia Pastoris ◽  
Fed Batch Fermentation
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