scholarly journals Enhanced production of mouse α-amylase by feeding combined nitrogen and carbon sources in fed-batch culture of recombinant Pichia pastoris

2006 ◽  
Vol 41 (2) ◽  
pp. 390-397 ◽  
Author(s):  
Du Bok Choi ◽  
Enoch Y. Park
Keyword(s):  
Pichia Pastoris ◽  
Batch Culture ◽  
Carbon Sources ◽  
Fed Batch ◽  
Combined Nitrogen ◽  
Enhanced Production ◽  
Recombinant Pichia Pastoris ◽  
Fed Batch Culture

Two-stage glycerol feeding for enhancement of recombinant hG-CSF production in a fed-batch culture of Pichia pastoris

2008 ◽  
Vol 30 (6) ◽  
pp. 1081-1085 ◽  
Author(s):  
A. Bahrami ◽  
S. A. Shojaosadati ◽  
R. Khalilzadeh ◽  
E. Vasheghani Farahani
Keyword(s):  
Pichia Pastoris ◽  
Batch Culture ◽  
Fed Batch ◽  
Two Stage ◽  
Fed Batch Culture ◽  
Csf Production ◽  
Glycerol Feeding

scholarly journals Cultivation Method Effect on Schizochytrium sp. Biomass Growth and Docosahexaenoic Acid (DHA) Production with the Use of Waste Glycerol as a Source of Organic Carbon

Energies ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2952
Author(s):  
Natalia Kujawska ◽  
Szymon Talbierz ◽  
Marcin Dębowski ◽  
Joanna Kazimierowicz ◽  
Marcin Zieliński
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Batch Culture ◽  
Acid Production ◽  
Carbon Sources ◽  
Fed Batch ◽  
Waste Glycerol ◽  
Total Fatty Acids ◽  
Fed Batch Culture ◽  
Dha Production

Inexpensive carbon sources offering an alternative to glucose are searched for to reduce costs of docosahexaenoic acid production by microalgae. The use of waste glycerol seems substantiated and prospective in this case. The objective of this study was to determine the production yield of heterotrophic microalgae Schizochytrium sp. biomass and the efficiency of docosahexaenoic acid production in various types of cultures with waste glycerol. Cultivation conditions were optimized using the Plackett–Burman method and Response Surface Methodology. The highest technological performance was obtained in the fed-batch culture, where the concentration of Schizochytrium sp. biomass reached 103.44 ± 1.50 g/dm3, the lipid concentration in Schizochytrium sp. biomass was at 48.85 ± 0.81 g/dm3, and the docosahexaenoic acid concentration at 21.98 ± 0.36 g/dm3. The highest docosahexaenoic acid content, accounting for 61.76 ± 3.77% of total fatty acids, was determined in lipid bodies of the Schizochytrium sp. biomass produced in the batch culture, whereas the lowest one, accounting for 44.99 ± 2.12% of total fatty acids, in those of the biomass grown in the fed-batch culture.

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