scholarly journals Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor

2020 ◽  
Vol 8 ◽  
Author(s):  
Katsuaki Nitta ◽  
Francesco Del Carratore ◽  
Rainer Breitling ◽  
Eriko Takano ◽  
Sastia P. Putri ◽  
...  
Keyword(s):  
Streptomyces Coelicolor ◽  
Omics Analysis ◽  
Actinorhodin Production

scholarly journals Role of Phosphopantetheinyl Transferase Genes in Antibiotic Production by Streptomyces coelicolor

2008 ◽  
Vol 190 (20) ◽  
pp. 6903-6908 ◽  
Author(s):  
Ya-Wen Lu ◽  
Adrianna K. San Roman ◽  
Amy M. Gehring
Keyword(s):  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Morphological Development ◽  
Phosphopantetheinyl Transferase ◽  
Calcium Dependent ◽  
Actinorhodin Production

ABSTRACT The phosphopantetheinyl transferase genes SCO5883 (redU) and SCO6673 were disrupted in Streptomyces coelicolor. The redU mutants did not synthesize undecylprodigiosin, while SCO6673 mutants failed to produce calcium-dependent antibiotic. Neither gene was essential for actinorhodin production or morphological development in S. coelicolor, although their mutation could influence these processes.

Effect of inoculum type on actinorhodin production by Streptomyces coelicolor A3(2)

1995 ◽  
Vol 17 (6) ◽  
pp. 579-582 ◽  
Author(s):  
M. Elibol ◽  
K. Ulgen ◽  
K. Kamaruddin ◽  
F. Mavituna
Keyword(s):  
Streptomyces Coelicolor ◽  
Inoculum Type ◽  
Actinorhodin Production

An aberrant protein synthesis activity is linked with antibiotic overproduction in rpsL mutants of Streptomyces coelicolor A3(2)

Microbiology ◽  
2003 ◽  
Vol 149 (11) ◽  
pp. 3299-3309 ◽  
Author(s):  
Yoshiko Okamoto-Hosoya ◽  
Takeshi Hosaka ◽  
Kozo Ochi
Keyword(s):  
Protein Synthesis ◽  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Translation System ◽  
In The Wild ◽  
Synthesis Activity ◽  
Aberrant Protein ◽  
Ribosomal Protein S12 ◽  
High Level ◽  
Actinorhodin Production

Certain mutations in the rpsL gene (encoding the ribosomal protein S12) activate or enhance antibiotic production in various bacteria. K88E and P91S rpsL mutants of Streptomyces coelicolor A3(2), with an enhanced actinorhodin production, were found to exhibit an aberrant protein synthesis activity. While a high level of this activity (as determined by the incorporation of labelled leucine) was detected at the late stationary phase in the mutants, it decreased with age of the cells in the wild-type strain. In addition, the aberrant protein synthesis was particularly pronounced when cells were subjected to amino acid shift-down, and was independent of their ability to accumulate ppGpp. Ribosomes of K88E and P91S mutants displayed an increased accuracy in protein synthesis as demonstrated by the poly(U)-directed cell-free translation system, but so did K43N, K43T, K43R and K88R mutants, which were streptomycin resistant but showed no effect on actinorhodin production. This eliminates the possibility that the increased accuracy level is a cause of the antibiotic overproduction in the K88E and P91S mutants. The K88E and P91S mutant ribosomes exhibited an increased stability of the 70S complex under low concentrations of magnesium. The authors propose that the aberrant activation of protein synthesis caused by the increased stability of the ribosome is responsible for the remarkable enhancement of antibiotic production in the K88E and P91S mutants.

scholarly journals Transcriptome Analysis of an Antibiotic Downregulator Mutant and Synergistic Actinorhodin Stimulation via Disruption of a Precursor Flux Regulator inStreptomyces coelicolor

2011 ◽  
Vol 77 (5) ◽  
pp. 1872-1877 ◽  
Author(s):  
Seon-Hye Kim ◽  
Han-Na Lee ◽  
Hye-Jin Kim ◽  
Eung-Soo Kim
Keyword(s):  
Microarray Analysis ◽  
Transcriptome Analysis ◽  
Carbon Flux ◽  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Actinorhodin Production

ABSTRACTThrough microarray analysis of an antibiotic-downregulator-deletedStreptomyces coelicolorΔwblAΔSCO1712 mutant, 28wblA- and SCO1712-dependent genes were identified and characterized. Among 14wblA- and SCO1712-independent genes, a carbon flux regulating 6-phosphofructokinase SCO5426 was additionally disrupted in the ΔwblAΔSCO1712 mutant and further stimulated actinorhodin production inS. coelicolor, implying that both regulatory and precursor flux pathways could be synergistically optimized for antibiotic production.

scholarly journals Enzyme-Constrained Models and Omics Analysis of Streptomyces coelicolor Reveal Metabolic Changes that Enhance Heterologous Production

iScience ◽  
2020 ◽  
Vol 23 (9) ◽  
pp. 101525 ◽  
Author(s):  
Snorre Sulheim ◽  
Tjaša Kumelj ◽  
Dino van Dissel ◽  
Ali Salehzadeh-Yazdi ◽  
Chao Du ◽  
...  
Keyword(s):  
Streptomyces Coelicolor ◽  
Metabolic Changes ◽  
Heterologous Production ◽  
Omics Analysis ◽  
Constrained Models

Deletion of TerD-domain-encoding genes: effect on Streptomyces coelicolor development

2012 ◽  
Vol 58 (10) ◽  
pp. 1221-1229 ◽  
Author(s):  
Édith Sanssouci ◽  
Sylvain Lerat ◽  
François Daigle ◽  
Gilles Grondin ◽  
François Shareck ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Potential Role ◽  
Solid Medium ◽  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Developmental Patterns ◽  
Small Colony ◽  
Encoding Genes ◽  
Actinorhodin Production

TerD-domain-encoding genes (tdd genes) are highly represented in the Streptomyces coelicolor genome. One of these, the tdd8 gene, was recently shown to have a crucial influence on growth, differentiation, and spore development of this filamentous bacterium. The investigation of the potential role of tdd genes has been extended here to tdd7 (SCO2367) and tdd13 (SCO4277). Both genes are highly expressed in bacteria grown in liquid-rich medium (tryptic soy broth). However, the deletion of these genes in S. coelicolor showed contrasting effects regarding developmental patterns, sporulation, and antibiotic production. Deletion of the tdd7 gene induced a reduction of growth in liquid medium, wrinkling of the mycelium on solid medium, and poor spore and actinorhodin production. On the other hand, deletion of the tdd13 gene did not significantly affect growth in liquid medium but induced a small colony phenotype on solid medium with abundant sporulation and overproduction of undecylprodigiosin. Although their exact functions remain undefined, the present data suggest a major involvement of TerD proteins in the proper development of S. coelicolor.

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