scholarly journals The Independent Biological Activity of Bacillus thuringiensis Cry23Aa Protein Against Cylas puncticollis

2020 ◽  
Vol 11 ◽  
Author(s):  
Patricia Hernández-Martínez ◽  
Ayda Khorramnejad ◽  
Katterine Prentice ◽  
Ascensión Andrés-Garrido ◽  
Natalia Mara Vera-Velasco ◽  
...  
2018 ◽  
Vol 63 (2) ◽  
pp. 293-302
Author(s):  
You Kyoung LEE ◽  
Na Young JIN ◽  
Hee Ji KIM ◽  
Yong Man YOUN ◽  
Chisa YASUNAGA–AOKI ◽  
...  

2018 ◽  
Vol 111 (3) ◽  
pp. 1486-1489 ◽  
Author(s):  
Liliane Nachtigall Martins ◽  
Ana Paula de Souza Stori de Lara ◽  
Márcio Soares Ferreira ◽  
Adrise Medeiros Nunes ◽  
Daniel Bernardi ◽  
...  

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Dhekra Mhalla ◽  
Dalel Ben Farhat-Touzri ◽  
Slim Tounsi ◽  
Mohamed Trigui

The increasing insect resistance against Bacillus thuringiensis delta-endotoxins is a serious problem which makes it urgent to look for new eco-friendly strategies. Combining these toxins with other biomolecules is one of the promising strategies against insect pests. In this work, we evaluated the bioinsecticidal potential of Rumex tingitanus extracts and B. thuringiensis strain BLB250 against Spodoptera littoralis (Lepidoptera: Noctuidae) larvae. The chemical composition of the hexane extract, the most active fraction, was analyzed to validate the correlation between chemical composition and biological activity. Among the tested extracts, only the hexanic extract showed toxicity against first and second instar larvae with LC50 of 2.56 and 2.95 mg g−1, respectively. The Bacillus thuringiensis BLB250 delta-endotoxins showed toxicity with an LC50 of 56.3 μg g−1. Therefore, the investigated combinational effect of BLB250 delta-endotoxins and R. tingitanus hexane extract proved significant synergistic effect against S. littoralis larvae. The GC-MS analysis of R. tingitanus hexane extract showed the richness of this extract in phytosterols such as β and γ-sitosterol (48.91%), campesterol (6.43%), and β-amyrin (8.92%) which are known for their insecticidal activity. This novel finding highlights the potential use of this combination against insect pests to prevent the appearance of resistance problems.


Toxicon ◽  
2016 ◽  
Vol 122 ◽  
pp. 50-53 ◽  
Author(s):  
Patricia Hernández-Martínez ◽  
Natalia Mara Vera-Velasco ◽  
Baltasar Escriche

2016 ◽  
Vol 109 (3) ◽  
pp. 1071-1078 ◽  
Author(s):  
J Cossentine ◽  
M Robertson ◽  
D Xu

Abstract Whole-culture extracts of Bacillus thuringiensis Berliner strains were assayed against larval and adult Drosophila suzukii (Matsumura), an important invasive pest of many thin-skinned soft fruit crops in North America. Of the 22 serovars tested versus larval D. suzukii , strains of Bacillus thuringiensis var. thuringiensis , kurstaki , thompsoni , bolivia , and pakistani caused high (75 to 100%) first-instar mortalities. Pupal mortality, measured as a failure of adults to emerge, varied with serovar. The first D. suzukii instar was the most susceptible of the three larval instars to B. thuringiensis var. kurstaki HD-1. Larval D. suzukii are shielded from crop treatments, as they develop under the skin of infested fruit, and adults would be a more vulnerable target for an efficacious strain of B. thuringiensis . Only one of the 21 B. thuringiensis serovars, var. thuringiensis , prepared as oral suspensions in sucrose for adult D. suzukii ingestion resulted in significant, albeit low mortality within 7 d. It is not a candidate for use in pest management, as it produces β -exotoxin that is toxic to vertebrates.


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