A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes

Here, we report a novel double-stranded RNA virus designated Colletotrichum liriopes partitivirus 1 (ClPV1) from the plant pathogenic fungus C. liriopes. ClPV1 genome has two double stranded RNAs (dsRNAs), named as dsRNA 1 and dsRNA 2, which in the lengths of 1,807 and 1,706 bp, respectively. The dsRNA 1 and dsRNA 2 encoded proteins showing significant amino acid (aa) sequence identity to the RNA-dependent RNA polymerase (RdRp) and coat protein (CP) of partitiviruses, respectively. Phylogenetic analysis using the aa sequences of RdRp and CP indicated that ClPV1 was grouped to members of the putative Epsilonpartitivirus genus in the Partitiviridae family. Spherical viral particles in approximately 35 nm in diameter and packaging the ClPV1 genome were isolated. Virus elimination and virus transfection with purified viral particles, and biological comparison revealed that ClPV1 could reduce the virulence and conidia production of C. liriopes. To the best of our knowledge, this is the first report of mycovirus in C. liriopes fungus.

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Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani

Plant Pathology - Methods in Molecular Biology ◽

10.1007/978-1-59745-062-1_14 ◽

2008 ◽

pp. 171-182

Author(s):

Nikki D. Charlton ◽

Stellos M. Tavantzis ◽

Marc A. Cubeta

Keyword(s):

Rhizoctonia Solani ◽

Pathogenic Fungus ◽

Double Stranded Rna ◽

Plant Pathogenic Fungus ◽

Rna Elements

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RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.89.6.2185 ◽

1992 ◽

Vol 89 (6) ◽

pp. 2185-2189 ◽

Cited By ~ 24

Author(s):

J. C. Ribas ◽

R. B. Wickner

Keyword(s):

Rna Polymerase ◽

Rna Virus ◽

Consensus Sequence ◽

Rna Dependent Rna Polymerase ◽

Double Stranded Rna ◽

Definition Of

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Functions of Conserved Motifs in the RNA-Dependent RNA Polymerase of a Yeast Double-Stranded RNA Virus

Journal of Virology ◽

10.1128/jvi.72.5.4427-4429.1998 ◽

1998 ◽

Vol 72 (5) ◽

pp. 4427-4429 ◽

Cited By ~ 19

Author(s):

Eric Routhier ◽

Jeremy A. Bruenn

Keyword(s):

Saccharomyces Cerevisiae ◽

Rna Polymerase ◽

Rna Virus ◽

Rna Dependent Rna Polymerase ◽

Conserved Motifs ◽

Double Stranded Rna ◽

Conserved Regions

ABSTRACT At least eight conserved motifs are visible in the totivirus RNA-dependent RNA polymerase (RDRP). We have systematically altered each of these in the Saccharomyces cerevisiaedouble-stranded RNA virus ScVL1 by substituting the conserved motifs from a giardiavirus. The results help define the conserved regions of the RDRP involved in polymerase function and those essential for other reasons.

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A double-stranded RNA mycovirus from the plant pathogenic fungus,Fusarium solanif. sp.robiniae

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.1993.tb06312.x ◽

1993 ◽

Vol 110 (2) ◽

pp. 153-157 ◽

Cited By ~ 14

Author(s):

M. Nogawa ◽

M. Shimosaka ◽

T. Kageyama ◽

M. Okazaki

Keyword(s):

Pathogenic Fungus ◽

Double Stranded Rna ◽

Plant Pathogenic Fungus

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Replication of double-stranded RNA in mycovirus from the plant pathogenic fungus,Fusarium solani

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.1996.tb08080.x ◽

1996 ◽

Vol 137 (1) ◽

pp. 45-49 ◽

Cited By ~ 2

Author(s):

Masahiro Nogawa ◽

Aiko Nakatani ◽

Keiichi Gonda ◽

Makoto Shimosaka ◽

mitsuo Okazaki

Keyword(s):

Fusarium Solani ◽

Pathogenic Fungus ◽

Double Stranded Rna ◽

Plant Pathogenic Fungus

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Comparison of capsid-associated RNA-dependent RNA polymerase activity of double-stranded RNA virus-like particles from different yeast genera

Biochemical Society Transactions ◽

10.1042/bst022337s ◽

1994 ◽

Vol 22 (3) ◽

pp. 337S-337S ◽

Cited By ~ 1

Author(s):

RICHARD J JEWERS

Keyword(s):

Rna Polymerase ◽

Rna Virus ◽

Polymerase Activity ◽

Rna Dependent Rna Polymerase ◽

Double Stranded Rna ◽

Virus Like Particles ◽

Rna Polymerase Activity

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Late Male-Killing Viruses in Homona magnanima Identified as Osugoroshi Viruses, Novel Members of Partitiviridae

Frontiers in Microbiology ◽

10.3389/fmicb.2020.620623 ◽

2021 ◽

Vol 11 ◽

Author(s):

Ryosuke Fujita ◽

Maki N. Inoue ◽

Takumi Takamatsu ◽

Hiroshi Arai ◽

Mayu Nishino ◽

...

Keyword(s):

Mathematical Modeling ◽

Rna Polymerase ◽

Rna Viruses ◽

Rna Virus ◽

Horizontal Transmission ◽

Rna Dependent Rna Polymerase ◽

Double Stranded Rna ◽

First Report ◽

Male Killing ◽

Male Specific

Late male-killing, a male-specific death after hatching, is a unique phenotype found in Homona magnanima, oriental tea tortrix. The male-killing agent was suspected to be an RNA virus, but details were unknown. We herein successfully isolated and identified the putative male-killing virus as Osugoroshi viruses (OGVs). The three RNA-dependent RNA polymerase genes detected were phylogenetically related to Partitiviridae, a group of segmented double-stranded RNA viruses. Purified dsRNA from a late male-killing strain of H. magnanima revealed 24 segments, in addition to the RdRps, with consensus terminal sequences. These segments included the previously found male-killing agents MK1068 (herein OGV-related RNA16) and MK1241 (OGV-related RNA7) RNAs. Ultramicroscopic observation of purified virions, which induced late male-killing in the progeny of injected moths, showed sizes typical of Partitiviridae. Mathematical modeling showed the importance of late male-killing in facilitating horizontal transmission of OGVs in an H. magnanima population. This study is the first report on the isolation of partiti-like virus from insects, and one thought to be associated with late male-killing, although the viral genomic contents and combinations in each virus are still unknown.

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