scholarly journals Real-Time Monitoring of the Yeast Intracellular State During Bioprocesses With a Toolbox of Biosensors

2022 ◽  
Vol 12 ◽  
Author(s):  
Luca Torello Pianale ◽  
Peter Rugbjerg ◽  
Lisbeth Olsson

Industrial fermentation processes strive for high robustness to ensure optimal and consistent performance. Medium components, fermentation products, and physical perturbations may cause stress and lower performance. Cellular stress elicits a range of responses, whose extracellular manifestations have been extensively studied; whereas intracellular aspects remain poorly known due to lack of tools for real-time monitoring. Genetically encoded biosensors have emerged as promising tools and have been used to improve microbial productivity and tolerance toward industrially relevant stresses. Here, fluorescent biosensors able to sense the yeast intracellular environment (pH, ATP levels, oxidative stress, glycolytic flux, and ribosome production) were implemented into a versatile and easy-to-use toolbox. Marker-free and efficient genome integration at a conserved site on chromosome X of Saccharomyces cerevisiae strains and a commercial Saccharomyces boulardii strain was developed. Moreover, multiple biosensors were used to simultaneously monitor different intracellular parameters in a single cell. Even when combined together, the biosensors did not significantly affect key physiological parameters, such as specific growth rate and product yields. Activation and response of each biosensor and their interconnection were assessed using an advanced micro-cultivation system. Finally, the toolbox was used to screen cell behavior in a synthetic lignocellulosic hydrolysate that mimicked harsh industrial substrates, revealing differences in the oxidative stress response between laboratory (CEN.PK113-7D) and industrial (Ethanol Red) S. cerevisiae strains. In summary, the toolbox will allow both the exploration of yeast diversity and physiological responses in natural and complex industrial conditions, as well as the possibility to monitor production processes.

2019 ◽  
Vol 10 (8) ◽  
pp. 983-990 ◽  
Author(s):  
Junhuai Xu ◽  
Bin Yan ◽  
Xiaosheng Du ◽  
Junjie Xiong ◽  
Mi Zhou ◽  
...  

A TPE-based polyurethane prodrug has been established for mitochondria-targeting drug delivery and real-time monitoring.


Author(s):  
Lynn E. Secondo ◽  
Vitaliy Avrutin ◽  
Umit Ozgur ◽  
Erdem Topsakal ◽  
Nastassja A. Lewinski

2014 ◽  
Vol 134 (6) ◽  
pp. 1701-1709 ◽  
Author(s):  
Alexander M. Wolf ◽  
Kiyomi Nishimaki ◽  
Naomi Kamimura ◽  
Shigeo Ohta

2006 ◽  
Vol 175 (4S) ◽  
pp. 521-521
Author(s):  
Motoaki Saito ◽  
Tomoharu Kono ◽  
Yukako Kinoshita ◽  
Itaru Satoh ◽  
Keisuke Satoh

2001 ◽  
Vol 11 (PR3) ◽  
pp. Pr3-1175-Pr3-1182 ◽  
Author(s):  
M. Losurdo ◽  
A. Grimaldi ◽  
M. Giangregorio ◽  
P. Capezzuto ◽  
G. Bruno

2014 ◽  
Author(s):  
Rozaimi Ghazali ◽  
◽  
Asiah Mohd Pilus ◽  
Wan Mohd Bukhari Wan Daud ◽  
Mohd Juzaila Abd Latif ◽  
...  

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 101-LB
Author(s):  
ABHINAV BHUSHAN ◽  
SONALI J. KARNIK

2017 ◽  
Vol 4 (4) ◽  
pp. 20
Author(s):  
KAVITHA T. ◽  
PREETHI D. LAVANYA ◽  
SARANYA S. ◽  
ANCY EVERT P.M. JONAH ◽  
◽  
...  

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