scholarly journals Clinical Diagnosis of Red Cell Membrane Disorders: Comparison of Osmotic Gradient Ektacytometry and Eosin Maleimide (EMA) Fluorescence Test for Red Cell Band 3 (AE1, SLC4A1) Content for Clinical Diagnosis

2020 ◽  
Vol 11 ◽  
Author(s):  
Ahmar Urooj Zaidi ◽  
Steven Buck ◽  
Manisha Gadgeel ◽  
Miguel Herrera-Martinez ◽  
Araathi Mohan ◽  
...  

Author(s):  
Cathleen Magowan ◽  
Wataru Nunomura ◽  
Karena L. Waller ◽  
Jackson Yeung ◽  
Joy Liang ◽  
...  




1984 ◽  
Vol 124 (2) ◽  
pp. 437-442 ◽  
Author(s):  
Edward J. Victoria ◽  
Jeanine E. Kleeman ◽  
S.P. Masouredis


1989 ◽  
Vol 92 (4) ◽  
pp. 691-699
Author(s):  
A.R. Dluzewski ◽  
P.R. Fryer ◽  
S. Griffiths ◽  
R.J. Wilson ◽  
W.B. Gratzer

Immuno-gold labelling electron microscopy of thin sections was used to determine the distribution of red cell membrane and membrane skeleton proteins in the vicinity of internalized malaria parasites. When examined immediately after invasion (young ring-stage parasites), the parasitophorous vacuole membranes of both Plasmodium falciparum and P. knowlesi were found to be characterized by the essentially complete absence of spectrin, ankyrin and the most abundant transmembrane protein, band 3. P. knowlesi merozoites were trapped in the attached but not internalized state by pretreatment with cytochalasin B. In this merozoite-red cell complex antibody labelling showed that band 3 had been eliminated from the region of the host cell membrane in contact with the parasite. Internal vesicles, originating apparently from the site of attachment, were often observed in the red cell. Opposite the attached parasite a cavity was also sometimes seen in the host cell, presumably representing an incipient internal vesicle. The membrane was intact, as judged by the absence of protein (haemoglobin) in the cavity, and, like the membranes surrounding the internal vesicles, was devoid of membrane proteins. A large multilamellar body was sometimes seen in the merozoite close to its point of attachment. The lamellar spacing was about 50 nm. The electron microscope images suggest a diffusion of electron-dense material from the lamellar body into the cavity in the host cell.







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