scholarly journals OsIAA18, an Aux/IAA Transcription Factor Gene, Is Involved in Salt and Drought Tolerance in Rice

2021 ◽  
Vol 12 ◽  
Author(s):  
Feibing Wang ◽  
Haofei Niu ◽  
Dongqing Xin ◽  
Yi Long ◽  
Guangpeng Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Constitutive Expression ◽  
Ros Scavenging ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Rice Plants ◽  
Salt And Drought Tolerance ◽  
Drought And Salt Tolerance ◽  
Salt And Drought Stresses

Auxin/indoleacetic acid (Aux/IAA) proteins play an important regulatory role in the developmental process of plants and their responses to stresses. A previous study has shown that constitutive expression of OsIAA18, an Aux/IAA transcription factor gene of rice improved salt and osmotic tolerance in transgenic Arabidopsis plants. However, little work is known about the regulatory functions of the OsIAA18 gene in regulating the abiotic stress tolerance of rice. In this study, the OsIAA18 gene was introduced into the rice cultivar, Zhonghua 11 and the OsIAA18 overexpression in rice plants exhibited significantly enhanced salt and drought tolerance compared to the wild type (WT). Moreover, overexpression of OsIAA18 in rice increased endogenous levels of abscisic acid (ABA) and the overexpression of OsIAA18 in rice plants showed hypersensitivity to exogenous ABA treatment at both the germination and postgermination stages compared to WT. Overexpression of OsIAA18 upregulated the genes involved in ABA biosynthesis and signaling pathways, proline biosynthesis pathway, and reactive oxygen species (ROS)-scavenging system in the overexpression of OsIAA18 in rice plants under salt and drought stresses. Proline content, superoxide dismutase (SOD), and peroxidase (POD) activities were significantly increased, whereas malonaldehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion radical (O2–) content were significantly decreased in the transgenic plants under salt and drought stresses. Taken together, we suggest that OsIAA18 plays a positive role in drought and salt tolerance by regulating stress-induced ABA signaling. The OsIAA18 gene has a potential application in genetically modified crops with enhanced tolerance to abiotic stresses.

scholarly journals A novel maspardin protein gene OsMas is involved in salt and drought tolerance in rice

2021 ◽  
Author(s):  
Feibing Wang ◽  
Haofei Niu ◽  
Dongqing Xin ◽  
Guokun Tang ◽  
Yang Li ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Abiotic Stresses ◽  
Oryza Sativa L ◽  
Rice Cultivar ◽  
Crop Productivity ◽  
Ros Scavenging ◽  
Rice Plants ◽  
Salt And Drought Tolerance ◽  
Drought And Salt Tolerance ◽  
Salt And Drought Stresses

Abstract Drought and salt stresses, as major environmental abiotic stresses in agricultural worldwide, affect plant growth and crop productivity and quality. The development of crops with higher drought and salt tolerance is therefore highly desirable. Here, we reported the isolation and biological function and molecular characterization of a novel maspardin gene, OsMas, from rice cultivar Yangjing 805 (Oryza sativa L.). The expression levels of OsMas were up-regulated under mannitol, PEG6000, NaCl and ABA treatments in rice. Heterologous expression of OsMas enhanced salt and drought tolerance in Escherichia coli and Arabidopsis. Moreover, the OsMas gene was introduced into rice cultivar Zhonghua 11 (wild-type, WT) and the OsMas-overexpression (OsMas-OE) rice plants exhibited significantly enhanced salt and drought tolerance, while the OsMas-interference (OsMas-RNAi) rice plants exhibited decreased tolerance to salt and drought stresses, compared with WT plants. The OsMas-OE plants exhibited enhanced hypersensitive, while the OsMas-RNAi plants showed less sensitive to exogenous ABA treatment at both germination and post-germination stages. The content of ABA, proline and K+ and the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and photosynthesis were significantly increased, while the content of malonaldehyde (MDA), hydrogen peroxide (H2O2), superoxide anion radical (O2-) and Na+ were significantly decreased in OsMas-OE plants compared with OsMas-RNAi and WT plants. Overexpression of OsMas up-regulated the genes involved in ABA biosynthesis and signaling pathways, proline biosynthesis pathway, ROS-scavenging system, photosynthesis and ion transport pathways under salt and drought stresses. Collectively, our results indicate that the OsMas gene functions in improving salt and drought tolerance in rice, which may serve as a candidate gene for use in enhancing the resistance to abiotic stresses in crops.

scholarly journals A R2R3-MYB Transcription Factor Gene, BpMYB123, Regulates BpLEA14 to Improve Drought Tolerance in Betula platyphylla

2021 ◽  
Vol 12 ◽  
Author(s):  
Kaiwen Lv ◽  
Hairong Wei ◽  
Guifeng Liu
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Crop Production ◽  
Myb Transcription Factor ◽  
Betula Platyphylla ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Negative Impacts ◽  
R2r3 Myb

Drought stress causes various negative impacts on plant growth and crop production. R2R3-MYB transcription factors (TFs) play crucial roles in the response to abiotic stress. However, their functions in Betula platyphylla haven’t been fully investigated. In this study, a R2R3 MYB transcription factor gene, BpMYB123, was identified from Betula platyphylla and reveals its significant role in drought stress. Overexpression of BpMYB123 enhances tolerance to drought stress in contrast to repression of BpMYB123 by RNA interference (RNAi) in transgenic experiment. The overexpression lines increased peroxidase (POD) and superoxide dismatase (SOD) activities, while decreased hydrogen peroxide (H2O2), superoxide radicals (O2–), electrolyte leakage (EL) and malondialdehyde (MDA) contents. Our study showed that overexpression of BpMYB123 increased BpLEA14 gene expression up to 20-fold due to BpMYB123 directly binding to the MYB1AT element of BpLEA14 promoter. These results indicate that BpMYB123 acts as a regulator via regulating BpLEA14 to improve drought tolerance in birch.

scholarly journals Increased cuticular wax accumulation and enhanced drought tolerance in transgenic alfalfa (Medicago sativa) by overexpression of a transcription factor gene

2006 ◽  
Vol 12 ◽  
pp. 145-150
Author(s):  
J.I.-Yi Zhang ◽  
Mary Sledge ◽  
Joseph Bouton ◽  
Zeng-Y.U. Wang
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Medicago Sativa ◽  
Cuticular Wax ◽  
Transcription Factor Gene ◽  
Cuticular Waxes ◽  
Model Legume ◽  
Factor Gene ◽  
Transgenic Alfalfa ◽  
Ap2 Domain

Plant cuticular waxes play an important role in protecting aerial organs from damage caused by multiple environmental stresses such as drought, cold, UV radiation, pathogen infection, and insect attack. We characterized a novel AP2 domain-containing transcription factor gene, designated WXP1, from the model legume plant Medicago truncatula. The gene is able to activate wax production and confer drought tolerance in alfalfa (Medicago sativa). The predicted protein of WXP1 has 371 aa; it is one of the longest peptides of all the single AP2 domain proteins in M. truncatula. Transcript level of WXP1 is inducible by cold, ABA and drought treatment in shoot tissues. Overexpression of WXP1 under the control of CaMV35S promoter led to a significant increase in cuticular wax loading on leaves of transgenic alfalfa. Transgenic leaves showed reduced water loss and chlorophyll leaching. Transgenic alfalfa plants with increased cuticular waxes showed enhanced drought tolerance demonstrated by delayed wilting after watering was ceased and quicker and better recovery when the dehydrated plants were re-watered.

scholarly journals Characterization of Transcription Factor Gene OsDRAP1 Conferring Drought Tolerance in Rice

2018 ◽  
Vol 9 ◽  
Author(s):  
Liyu Huang ◽  
Yinxiao Wang ◽  
Wensheng Wang ◽  
Xiuqin Zhao ◽  
Qiao Qin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Transcription Factor Gene ◽  
Factor Gene
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