scholarly journals Overexpression of VyDOF8, a Chinese wild grapevine transcription factor gene, enhances drought tolerance in transgenic tobacco

2021 ◽  
pp. 104592
Author(s):  
Guirong Li ◽  
Wenwen Xu ◽  
Pengwei Jing ◽  
Xiaojin Hou ◽  
Xiucai Fan
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Wild Grapevine

scholarly journals Expression of MYB transcription factor gene ZmMYB59 affects seed germination in Nicotiana tabacum and Oryza sativa

2020 ◽  
Author(s):  
Kaihui Zhai ◽  
Guangwu Zhao ◽  
Hongye Jiang ◽  
Caixia Sun ◽  
Jingyu Ren
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Seed Germination ◽  
Transgenic Tobacco ◽  
Germination Rate ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Myb Transcription Factors ◽  
Vigor Index

Abstract Background MYB transcription factors are involved in many biological processes, including metabolism, development and responses to biotic and abiotic stresses. In our previous work, a new MYB transcription factor gene, ZmMYB59 was induced by deep sowing and down-regulated during maize seed germination via Real-Time PCR. However, there are few reports on seed germination regulated by MYB proteins and the functions of ZmMYB59 remain unknown. Results In this study, to examine its functions, Agrobacterium -mediated transformation was exploited to generate ZmMYB59 transgenic tobacco and rice. In T 2 generation transgenic tobacco, germination rate, germination index, vigor index and hypocotyl length were significantly decreased by 25.0~50.9%, 34.5~54.4%, 57.5~88.3% and 21.9~31.2% compared to wild-type (WT) lines. In T 2 generation transgenic rice, germination rate, germination index, vigor index and mesocotyl length were notably reduced by 39.1~53.8%, 51.4~71.4%, 52.5~74.0% and 28.3~41.5%, respectively. On this basis, relative physiological indicators were determined. The activities of catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and proline content of transgenic lines were significantly lower than those of WT, suggesting that ZmMYB59 reduced their antioxidant capacity. As well, ZmMYB59 expression extremely inhibited the synthesis of gibberellin A1 (GA 1 ) and cytokinin (CTK), and promoted the synthesis of abscisic acid (ABA) concurrently, which implied that seed germination was repressed by ZmMYB59 in hormone levels. Furthermore, cell length and cell number of hypocotyl/mesocotyl in transgenic plants were notably decreased. Conclusions Taken together, it proposed that ZmMYB59 plays a negative regulation during seed germination in tobacco and rice, which also contributes to illuminate the molecular mechanisms regulated by MYB transcription factors.

scholarly journals A R2R3-MYB Transcription Factor Gene, BpMYB123, Regulates BpLEA14 to Improve Drought Tolerance in Betula platyphylla

2021 ◽  
Vol 12 ◽  
Author(s):  
Kaiwen Lv ◽  
Hairong Wei ◽  
Guifeng Liu
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Crop Production ◽  
Myb Transcription Factor ◽  
Betula Platyphylla ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Negative Impacts ◽  
R2r3 Myb

Drought stress causes various negative impacts on plant growth and crop production. R2R3-MYB transcription factors (TFs) play crucial roles in the response to abiotic stress. However, their functions in Betula platyphylla haven’t been fully investigated. In this study, a R2R3 MYB transcription factor gene, BpMYB123, was identified from Betula platyphylla and reveals its significant role in drought stress. Overexpression of BpMYB123 enhances tolerance to drought stress in contrast to repression of BpMYB123 by RNA interference (RNAi) in transgenic experiment. The overexpression lines increased peroxidase (POD) and superoxide dismatase (SOD) activities, while decreased hydrogen peroxide (H2O2), superoxide radicals (O2–), electrolyte leakage (EL) and malondialdehyde (MDA) contents. Our study showed that overexpression of BpMYB123 increased BpLEA14 gene expression up to 20-fold due to BpMYB123 directly binding to the MYB1AT element of BpLEA14 promoter. These results indicate that BpMYB123 acts as a regulator via regulating BpLEA14 to improve drought tolerance in birch.

A R2R3-MYB transcription factor gene, FtMYB13, from Tartary buckwheat improves salt/drought tolerance in Arabidopsis

2018 ◽  
Vol 132 ◽  
pp. 238-248 ◽  
Author(s):  
Yunji Huang ◽  
Haixia Zhao ◽  
Fei Gao ◽  
Panfeng Yao ◽  
Renyu Deng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Tartary Buckwheat ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
R2r3 Myb

scholarly journals Increased cuticular wax accumulation and enhanced drought tolerance in transgenic alfalfa (Medicago sativa) by overexpression of a transcription factor gene

2006 ◽  
Vol 12 ◽  
pp. 145-150
Author(s):  
J.I.-Yi Zhang ◽  
Mary Sledge ◽  
Joseph Bouton ◽  
Zeng-Y.U. Wang
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Medicago Sativa ◽  
Cuticular Wax ◽  
Transcription Factor Gene ◽  
Cuticular Waxes ◽  
Model Legume ◽  
Factor Gene ◽  
Transgenic Alfalfa ◽  
Ap2 Domain

Plant cuticular waxes play an important role in protecting aerial organs from damage caused by multiple environmental stresses such as drought, cold, UV radiation, pathogen infection, and insect attack. We characterized a novel AP2 domain-containing transcription factor gene, designated WXP1, from the model legume plant Medicago truncatula. The gene is able to activate wax production and confer drought tolerance in alfalfa (Medicago sativa). The predicted protein of WXP1 has 371 aa; it is one of the longest peptides of all the single AP2 domain proteins in M. truncatula. Transcript level of WXP1 is inducible by cold, ABA and drought treatment in shoot tissues. Overexpression of WXP1 under the control of CaMV35S promoter led to a significant increase in cuticular wax loading on leaves of transgenic alfalfa. Transgenic leaves showed reduced water loss and chlorophyll leaching. Transgenic alfalfa plants with increased cuticular waxes showed enhanced drought tolerance demonstrated by delayed wilting after watering was ceased and quicker and better recovery when the dehydrated plants were re-watered.

scholarly journals Characterization of Transcription Factor Gene OsDRAP1 Conferring Drought Tolerance in Rice

2018 ◽  
Vol 9 ◽  
Author(s):  
Liyu Huang ◽  
Yinxiao Wang ◽  
Wensheng Wang ◽  
Xiuqin Zhao ◽  
Qiao Qin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Transcription Factor Gene ◽  
Factor Gene

Anthocyanin production by over-expression of grape transcription factor gene VlmybA2 in transgenic tobacco and Arabidopsis

2007 ◽  
Vol 1 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Sudarshanee Geekiyanage ◽  
Tomoyuki Takase ◽  
Yasunobu Ogura ◽  
Tomohiro Kiyosue
Keyword(s):  
Transcription Factor ◽  
Transgenic Tobacco ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Over Expression ◽  
Anthocyanin Production

Overexpression of a chrysanthemum transcription factor gene DgNAC1 improves drought tolerance in chrysanthemum

2018 ◽  
Vol 135 (1) ◽  
pp. 119-132 ◽  
Author(s):  
Qian Zhao ◽  
Ming Zhong ◽  
Ling He ◽  
Bei Wang ◽  
Qing-lin Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Transcription Factor Gene ◽  
Factor Gene
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