scholarly journals Membrane Protein Modified Electrodes in Bioelectrocatalysis

Catalysts ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1427
Author(s):  
Huijie Zhang ◽  
Rosa Catania ◽  
Lars J. C. Jeuken
Keyword(s):  
Membrane Protein ◽  
Energy Conversion ◽  
Redox Reactions ◽  
Artificial Photosynthesis ◽  
Transmembrane Proteins ◽  
Modified Electrodes ◽  
Future Directions ◽  
Common Electrode ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

Transmembrane proteins involved in metabolic redox reactions and photosynthesis catalyse a plethora of key energy-conversion processes and are thus of great interest for bioelectrocatalysis-based applications. The development of membrane protein modified electrodes has made it possible to efficiently exchange electrons between proteins and electrodes, allowing mechanistic studies and potentially applications in biofuels generation and energy conversion. Here, we summarise the most common electrode modification and their characterisation techniques for membrane proteins involved in biofuels conversion and semi-artificial photosynthesis. We discuss the challenges of applications of membrane protein modified electrodes for bioelectrocatalysis and comment on emerging methods and future directions, including recent advances in membrane protein reconstitution strategies and the development of microbial electrosynthesis and whole-cell semi-artificial photosynthesis.

scholarly journals Oriented Membrane Protein Reconstitution into Tethered Lipid Membranes for AFM Force Spectroscopy

2016 ◽  
Vol 111 (9) ◽  
pp. 1925-1934 ◽  
Author(s):  
Anna M. Bronder ◽  
Adeline Bieker ◽  
Shantha Elter ◽  
Manuel Etzkorn ◽  
Dieter Häussinger ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Lipid Membranes ◽  
Force Spectroscopy ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

scholarly journals Self-Assembly of Polymer-Encased Lipid Nanodiscs and Membrane Protein Reconstitution

2019 ◽  
Vol 123 (21) ◽  
pp. 4562-4570 ◽  
Author(s):  
Bikash R. Sahoo ◽  
Takuya Genjo ◽  
Kanhu C. Moharana ◽  
Ayyalusamy Ramamoorthy
Keyword(s):  
Membrane Protein ◽  
Self Assembly ◽  
Membrane Protein Reconstitution ◽  
Lipid Nanodiscs ◽  
Protein Reconstitution

scholarly journals A procedure for membrane-protein reconstitution and the functional reconstitution of the anion transport system of the human-erythrocyte membrane

1980 ◽  
Vol 189 (1) ◽  
pp. 35-44 ◽  
Author(s):  
J M Wolosin
Keyword(s):  
Membrane Protein ◽  
Anion Exchange ◽  
Exchange System ◽  
Protein Preparation ◽  
Protein Properties ◽  
Anion Transport System ◽  
Functional Features ◽  
Basic Features ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

A short procedure for the isolation of band-3 protein, the protein responsible for anion exchange in erythrocytes, in a reasonable degree of purity was developed. Using this protein preparation and a novel procedure for membrane-protein reconstitution, vesicles displaying the basic features of the anion-exchange system of the erythrocyte were obtained. The reconstitution procedure is based on slow direct removal of Triton X-100 from aqueous lipid/detergent solutions. According to the composition of the reconstitution medium, either small single-walled or large multi-walled vesicles are obtained. The procedure conserves protein properties well, as is revealed by the similarity of the rates of SO4(2-) exchange in erythrocytes and reconstituted vesicles when corrected for the relevant volumes. A number of functional features of the exchange system were studied and compared with those of the native membrane.

scholarly journals Membrane protein reconstitution and crystallization by controlled dilution

FEBS Letters ◽  
2003 ◽  
Vol 555 (1) ◽  
pp. 160-169 ◽  
Author(s):  
H.-W Rémigy ◽  
D Caujolle-Bert ◽  
K Suda ◽  
A Schenk ◽  
M Chami ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

scholarly journals Synthesis and characterization of tethered lipid assemblies for membrane protein reconstitution (Review)

2017 ◽  
Vol 12 (4) ◽  
pp. 04E301 ◽  
Author(s):  
Rémi Veneziano ◽  
Claire Rossi ◽  
Alexandre Chenal ◽  
Catherine Brenner ◽  
Daniel Ladant ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Synthesis And Characterization ◽  
Lipid Assemblies ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution

Frontal affinity chromatographic analysis of membrane protein reconstitution

1997 ◽  
Vol 4 (4) ◽  
pp. 221-226 ◽  
Author(s):  
Andreas Lundqvist ◽  
Eggert Brekkan ◽  
Christine Lagerquist ◽  
Lars Haneskog ◽  
Per Lundahi
Keyword(s):  
Membrane Protein ◽  
Chromatographic Analysis ◽  
Membrane Protein Reconstitution ◽  
Protein Reconstitution
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