scholarly journals The U94 Gene of Human Herpesvirus 6: A Narrative Review of Its Role and Potential Functions

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2608
Author(s):  
Elisabetta Caselli ◽  
Maria D’Accolti ◽  
Francesca Caccuri ◽  
Irene Soffritti ◽  
Valentina Gentili ◽  
...  
Keyword(s):  
Natural Infection ◽  
Human Herpesvirus ◽  
Cell Tropism ◽  
Human Herpesvirus 6 ◽  
Cell Level ◽  
Associated Diseases ◽  
Virus Life Cycle ◽  
Genome Homology ◽  
Herpesvirus 6 ◽  
Human Herpesviruses

Human herpesvirus 6 (HHV-6) is a β-herpesvirus that is highly prevalent in the human population. HHV-6 comprises two recognized species (HHV-6A and HHV-6B). Despite different cell tropism and disease association, HHV-6A/B show high genome homology and harbor the conserved U94 gene, which is limited to HHV-6 and absent in all the other human herpesviruses. U94 has key functions in the virus life cycle and associated diseases, having demonstrated or putative roles in virus replication, integration, and reactivation. During natural infection, U94 elicits an immune response, and the prevalence and extent of the anti-U94 response are associated with specific diseases. Notably, U94 can entirely reproduce some virus effects at the cell level, including inhibition of cell migration, induction of cytokines and HLA-G expression, and angiogenesis inhibition, supporting a direct U94 role in the development of HHV-6-associated diseases. Moreover, specific U94 properties, such as the ability to modulate angiogenesis pathways, have been exploited to counteract cancer development. Here, we review the information available on this key HHV-6 gene, highlighting its potential uses.

Detection of Human Herpesvirus 6 and Varicella-Zoster Virus in Tear Fluid of Patients with Bell's Palsy by PCR

2000 ◽  
Vol 38 (7) ◽  
pp. 2753-2755 ◽  
Author(s):  
A. Pitkäranta ◽  
H. Piiparinen ◽  
L. Mannonen ◽  
M. Vesaluoma ◽  
A. Vaheri
Keyword(s):  
Varicella Zoster Virus ◽  
Human Herpesvirus ◽  
Bell’S Palsy ◽  
Tear Fluid ◽  
Healthy Controls ◽  
Human Herpesvirus 6 ◽  
Bell's Palsy ◽  
Varicella Zoster ◽  
Herpesvirus 6 ◽  
Human Herpesviruses

Human herpesvirus 6 DNA was detected by PCR in the tear fluid of 7 (35%) of 20 patients with Bell's palsy and of 1 (5%) of 20 healthy controls. Varicella-zoster virus was detected by PCR in the tear fluid of 2 of 20 Bell's palsy patients but in none of the tear fluids from 20 healthy controls. These findings suggest an association between human herpesviruses and Bell's palsy.

Quantitative analysis of human herpesvirus 6 cell tropism

2004 ◽  
Vol 75 (1) ◽  
pp. 76-85 ◽  
Author(s):  
L. De Bolle ◽  
J. Van Loon ◽  
E. De Clercq ◽  
Lieve Naesens
Keyword(s):  
Quantitative Analysis ◽  
Human Herpesvirus ◽  
Cell Tropism ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6

scholarly journals Loss of maternally derived human herpesvirus-6 immunity and natural infection in argentinian infants

2001 ◽  
Vol 5 (4) ◽  
pp. 202-204 ◽  
Author(s):  
Lax A. Alessio ◽  
Silvia E. Carricart ◽  
Dolores Bustos ◽  
Silvia V. Nates ◽  
Hector Gendelman ◽  
...  
Keyword(s):  
Natural Infection ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6

scholarly journals Complementation of the Function of Glycoprotein H of Human Herpesvirus 6 Variant A by Glycoprotein H of Variant B in the Virus Life Cycle

2012 ◽  
Vol 86 (16) ◽  
pp. 8492-8498 ◽  
Author(s):  
Hiroko Oyaizu ◽  
Huamin Tang ◽  
Megumi Ota ◽  
Nobuyuki Takenaka ◽  
Keiichi Ozono ◽  
...  
Keyword(s):  
Human Herpesvirus ◽  
Artificial Chromosome ◽  
Amino Acid Identity ◽  
Human Herpesvirus 6 ◽  
Content Type ◽  
Glycoprotein Complex ◽  
Virus Life Cycle ◽  
Glycoprotein H ◽  
Herpesvirus 6 ◽  
Infectious Cycle

Human herpesvirus 6 (HHV-6) is a T-cell-tropic betaherpesvirus. HHV-6 can be classified into two variants, HHV-6 variant A (HHV-6A) and HHV-6B, based on genetic, antigenic, and cell tropisms, although the homology of their entire genomic sequences is nearly 90%. The HHV-6A glycoprotein complex gH/gL/gQ1/gQ2 is a viral ligand that binds to the cellular receptor human CD46. Because gH has 94.3% amino acid identity between the variants, here we examined whether gH from one variant could complement its loss in the other. Recently, we successfully reconstituted HHV-6A from its cloned genome in a bacterial artificial chromosome (BAC) (rHHV-6ABAC). Using this system, we constructed HHV-6ABAC DNA containing the HHV-6B gH (BgH) gene instead of the HHV-6A gH (AgH) gene inEscherichia coli. Recombinant HHV-6ABAC expressing BgH (rHHV-6ABAC-BgH) was successfully reconstituted. In addition, a monoclonal antibody that blocks HHV-6B but not HHV-6A infection neutralized rHHV-6ABAC-BgH but not rHHV-6ABAC. These results indicate that HHV-6B gH can complement the function of HHV-6A gH in the viral infectious cycle.

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